Mise en évidence d´un cas de «abortion storm» (transmission transplacentaire exogène de Neospora caninum) dans une exploitation de vaches laitières: une première en Suisse.

2005 ◽  
Vol 147 (3) ◽  
pp. 113-120 ◽  
Author(s):  
H. Sager ◽  
D. Hüssy ◽  
A. Kuffer ◽  
F. Schreve ◽  
B. Gottstein
Keyword(s):  
Neospora Caninum

SOROPREVALÊNCIA E FATORES DE RISCO PARA A INFECÇÃO POR Neospora caninum EM CÃES DE ZONA RURAL DO MUNICÍPIO DE SOUSA, PARAÍBA

2020 ◽  
Vol 4 (3) ◽  
pp. 42
Author(s):  
Samara Dos Santos Silva ◽  
Leonardo Vinícius Silva de Oliveira ◽  
Radabley Rith Almeida Oliveira ◽  
Emerson T De Alcântara Timóteo ◽  
Pollyanne Raysa Fernandes de Oliveira ◽  
...  
Keyword(s):  
Odds Ratio ◽  
Neospora Caninum ◽  
Font Size

<p><span style="font-size: medium;"><em>Neospora caninum </em>é um protozoário cujos hospedeiros definitivos são canídeos domésticos e silvestres, e hospedeiros intermediários são os herbívoros. Objetivou-se determinar a prevalência de anticorpos IgG anti-<em>N. caninum </em>em cães de zona rural do município de Sousa, Paraíba. Foram analisadas amostras de 98 cães de zona rural, através da Reação de Imuno fluorescência Indireta no ponto de corte 1:50. A frequência de soro positividade foi de 9,18% (9/98). A titulação de anticorpos variou de 50 a 200, predominando o título 1:50. Em 66,6% (4/6) das propriedades avaliadas havia pelo menos um cão soropositivo. O contato com bovinos foi considerado fator de risco (<em>Odds ratio</em>=15,25) para a infecção, demonstrando maior risco de contato com tecidos contaminados. Conclui-se que os cães de zona rural do município de Sousa estão expostos ao <em>N. caninum</em>. </span></p>

Seroprevalence of Antibodies to Neospora caninum in Urban and Rural Dogs in north-west Italy

2007 ◽  
Vol 54 (3-4) ◽  
pp. 135-139 ◽  
Author(s):  
E. Ferroglio ◽  
M. Pasino ◽  
F. Ronco ◽  
A. Benà ◽  
A. Trisciuoglio
Keyword(s):  
Neospora Caninum ◽  
North West

scholarly journals Spatial distance between sites of sampling associated with genetic variation among Neospora caninum in aborted bovine foetuses from northern Italy

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Luca Villa ◽  
Pavlo Maksimov ◽  
Christine Luttermann ◽  
Mareen Tuschy ◽  
Alessia L. Gazzonis ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Neospora Caninum ◽  
Geographic Distance ◽  
Rare Event ◽  
Northern Italy ◽  
Spatial Distance ◽  
Italian Regions ◽  
Bovine Abortion

Abstract Background Neospora caninum, a coccidian protozoan, represents an important cause of bovine abortion. Available N. caninum strains show considerable variation in vitro and in vivo, including different virulence in cattle. To which extent sexual recombination, which is possible in the intestines of domestic dogs and closely related carnivores as definitive hosts, contributes to this variation is not clear yet. Methods Aborted bovine foetuses were collected between 2015 and early 2019 from Italian Holstein Friesian dairy herds suffering from reproductive problems. A total of 198 samples were collected from 165 intensive farms located in Lombardy, northern Italy. N. caninum samples were subjected to multilocus-microsatellite genotyping using ten previously established microsatellite markers. In addition to our own data, those from a recent study providing data on five markers from other northern Italian regions were included and analysed. Results Of the 55 samples finally subjected to genotyping, 35 were typed at all or 9 out of 10 loci and their individual multilocus-microsatellite genotype (MLMG) determined. Linear regression revealed a statistically significant association between the spatial distance of the sampling sites with the genetic distance of N. caninum MLMGs (P < 0.001). Including data from this and a previous North Italian study into eBURST analysis revealed that several of N. caninum MLMGs from northern Italy separate into four groups; most of the samples from Lombardy clustered in one of these groups. Principle component analysis revealed similar clusters and confirmed MLMG groups identified by eBURST. Variations observed between MLMGs were not equally distributed over all loci, but predominantly observed in MS7, MS6A, or MS10. Conclusions Our findings confirm the concept of local N. caninum subpopulations. The geographic distance of sampling was associated with the genetic distance as determined by microsatellite typing. Results suggest that multi-parental recombination in N. caninum is a rare event, but does not exclude uniparental mating. More comprehensive studies on microsatellites in N. caninum and related species like Toxoplasma gondii should be undertaken, not only to improve genotyping capabilities, but also to understand possible functions of these regions in the genomes of these parasites.

scholarly journals Neospora caninum and/or Toxoplasma gondii Seroprevalence: Vaccination against PCV2 and Muscle Enzyme Activity in Seropositive and Seronegative Pigs

2021 ◽  
Vol 9 (5) ◽  
pp. 1097
Author(s):  
Labrini V. Athanasiou ◽  
Vasileios G. Papatsiros ◽  
Victoria M. Spanou ◽  
Eleni G. Katsogiannou ◽  
Anna Dedousi
Keyword(s):  
Enzyme Activity ◽  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Human Infection ◽  
Porcine Circovirus ◽  
Muscle Enzyme ◽  
Blood Samples ◽  
Zoonotic Pathogen ◽  
Muscle Enzyme Activity ◽  
Immunofluorescence Antibody

Neospora caninum and Toxoplasma gondii affect both humans and animals worldwide. To investigate their seroprevalence and differences in seropositivity between pigs vaccinated and unvaccinated against porcine circovirus 2 (PCV2), as well as differences in muscle enzyme activity between seropositive and seronegative pigs, blood samples were collected from 380 sows. Antibodies against T. gondii and N. caninum were detected by an indirect immunofluorescence antibody (IFA) assay, while the activities of creatine kinase (CK) and aspartate aminotransferase (AST) were biochemically assessed. Out of the 364 sows finally included in the study, 4.4%, 3.5%, and 0.5% were seropositive to T. gondii, N. caninum, or both. A significantly higher percentage of seropositivity against T. gondii and/or N. caninum in PCV2 unvaccinated pigs compared with vaccinated pigs was observed. Increased serum activities of CK and AST were detected in 71.43% and 100% of only against T. gondii (T+) and 63.64% and 90.91% of only against N. caninum (N+) seropositive sows, respectively, and were significantly higher compared to seronegative animals. T. gondii and N. caninum seropositivity, especially in presumed immunocompromised pigs, and the evidence of muscle damage highlight their importance as a zoonotic pathogen and animal model of human infection, respectively.

scholarly journals Evidence for Unknown Sarcocystis-Like Infection in Stranded Striped Dolphins (Stenella coeruleoalba) from the Ligurian Sea, Italy

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1201
Author(s):  
Federica Giorda ◽  
Umberto Romani-Cremaschi ◽  
Antoinette E. Marsh ◽  
Carla Grattarola ◽  
Barbara Iulini ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Positive Staining ◽  
Ligurian Sea ◽  
Sarcocystis Neurona ◽  
Stenella Coeruleoalba ◽  
Polyclonal Antisera ◽  
Ligurian Coast ◽  
Sarcocystis Falcatula ◽  
The Brain ◽  
The Mediterranean Basin

Two striped dolphins (SD1, SD2), stranded along the Ligurian coast of Italy, were diagnosed with a nonsuppurative meningoencephalitis associated with previously undescribed protozoan tissue cysts. As tissue cysts were morphologically different from those of Toxoplasma gondii, additional histopathological, immunohistochemical, ultrastructural, and biomolecular investigations were performed, aiming to fully characterize the organism. Histopathology revealed the presence of large Sarcocystis-like tissue cysts, associated with limited inflammatory lesions in all CNS areas studied. IHC was inconclusive, as positive staining with polyclonal antisera did not preclude cross-reaction with other Sarcocystidae coccidia. Applied to each animal, 11 different PCR protocols precluded a neural infection by Sarcocystis neurona, Sarcocystis falcatula, Hammondia hammondi, and Neospora caninum. T. gondii coinfection was confirmed only in dolphin SD2. Sarcocystis sp. sequences, showing the highest homology to species infecting the Bovidae family, were amplified from SD1 myocardium and SD2 skeletal muscle. The present study represents the first report of Sarcocystis-like tissue cysts in the brain of stranded cetaceans along with the first description of Sarcocystis sp. infection in muscle tissue of dolphins from the Mediterranean basin.

scholarly journals Biosurveillance of Selected Pathogens with Zoonotic Potential in a Zoo

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 428
Author(s):  
Pavel Kvapil ◽  
Joško Račnik ◽  
Marjan Kastelic ◽  
Jiřina Marková ◽  
Jean-Benjamin Murat ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Neospora Caninum ◽  
Field Mouse ◽  
Wild Rodents ◽  
Cygnus Olor ◽  
Multiplex Pcr Assay ◽  
Significant Difference ◽  
Zoo Animals ◽  
Mute Swans

Monitoring of infectious diseases is one of the most important pillars of preventive medicine in zoos. Screening for parasitic and bacterial infections is important to keep animals and equipment safe from pathogens that may pose a risk to animal and human health. Zoos usually contain many different animal species living in proximity with people and wild animals. As an epidemiological probe, 188 animals (122 mammals, 65 birds, and one reptile) from a zoo in Slovenia were examined for selected pathogens. Antibodies to Toxoplasma gondii and Neospora caninum were detected by ELISA in 38% (46/122) and 3% (4/122) of mammals, and in 0% (0/64) and 2% (1/57) of birds, respectively; the reptile (0/1) was negative. A statistically significant difference in T. gondii prevalence was found in Carnivora compared to Cetartiodactyla and primate antibodies to Encephalitozoon cuniculi were detected by IFAT in 44% (52/118) of mammals and 20% (11/56) of birds, respectively; the reptile (0/1) was negative. Herbivores had a higher chance of being infected with E. cuniculi compared to omnivores. Antibodies to Chlamydia abortus and Coxiella burnetii were not detected in any of the 74 tested zoo animals. The sera of 39 wild rodents found in the zoo were also examined; they were negative for all three parasites. The parasite T. gondii was detected by PCR in the tissue of two mute swans (Cygnus olor), three eastern house mice (Mus musculus), one yellow-necked field mouse (Apodemus flavicollis), and one striped field mouse (A. agrarius). Positive samples were genotyped by a single multiplex PCR assay using 15 microsatellite markers; one sample from a mute swan was characterized as type II. This micro-epidemiological study offers a better understanding of pathogens in zoo animals and an understanding of the role of zoos in biosurveillance.

scholarly journals Apicomplexan Protozoa Responsible for Reproductive Disorders: Occurrence of DNA in Blood and Milk of Donkeys (Equus asinus) and Minireview of the Related Literature

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 111
Author(s):  
Stefania Perrucci ◽  
Lisa Guardone ◽  
Iolanda Altomonte ◽  
Federica Salari ◽  
Simona Nardoni ◽  
...  
Keyword(s):  
Animal Species ◽  
Neospora Caninum ◽  
Reproductive Disorders ◽  
Theileria Equi ◽  
Blood Samples ◽  
Related Literature ◽  
Milk Samples ◽  
Equus Asinus ◽  
Time Periods ◽  
Blood Specimens

Donkeys may be susceptible to many pathological agents and may act as carriers of pathogens for other animal species and humans. This study evaluated the occurrence of potentially abortifacient apicomplexan protozoa DNA in blood and milk samples collected at different time periods during lactation (1, 6, and 10 months) from 33 healthy dairy jennies. A total of 73 blood and 73 milk samples were used for DNA extraction and analysis. Blood specimens from 11/33 (33%) jennies scored positive for Theileria equi, while milk samples scored negative. Blood and milk of 3/33 jennies yielded DNA of Toxoplasma gondii at 6 months (n. 1) and 10 months (n. 2) after parturition. Neospora caninum DNA was found in four milk and in five blood samples only at one month after parturition. This study is the first report about the presence of N. caninum DNA in milk of naturally infected jennies. Moreover, the excretion of N. caninum DNA in some of these jennies at 30 days from the parturition may suggest a possible occurrence of an endogenous cycle, while the presence of T. gondii DNA in the milk collected at 6 and 10 months after parturition may be suggestive of a discontinuous excretion.

Transplacental transmission of Neospora caninum to lambs in successive pregnancies of naturally infected sheep in Southern Brazil

2021 ◽  
Vol 23 ◽  
pp. 100537
Author(s):  
Kamila Alcalá Gonçalves Pereira ◽  
Renato Silva de Sousa ◽  
Mary Suzan Varaschin ◽  
Ana Paula Brenner Busch Becker ◽  
Alda Lúcia Gomes Monteiro ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Infected Sheep ◽  
Transplacental Transmission ◽  
Southern Brazil

Cytologic detection of Toxoplasma gondii in the cerebrospinal fluid of a dog and in vitro isolation of a unique mouse-virulent recombinant strain

2021 ◽  
pp. 104063872199668
Author(s):  
Waléria Borges-Silva ◽  
Mariana M. Rezende-Gondim ◽  
Gideão S. Galvão ◽  
Daniele S. Rocha ◽  
George R. Albuquerque ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Toxoplasma Gondii ◽  
Recombinant Strain ◽  
Neospora Caninum ◽  
Clinical Signs ◽  
Species Identity ◽  
Cytologic Examination ◽  
Pcr Rflp ◽  
Tissue Homogenates

Parasites resembling Neospora caninum or Toxoplasma gondii were detected by cytologic examination of cerebrospinal fluid (CSF) from a dog with neurologic disease. The dog became severely ill and was euthanized. Canine tissue homogenates were used for direct parasite isolation in cell culture, bioassay in 2 mouse lineages, and PCR. T. gondii was isolated in monkey kidney cells, and species identity was confirmed by PCR. Inoculated parasites were highly virulent for mice, which developed clinical signs and were euthanized immediately. PCR-RFLP for T. gondii using the cultured isolate (TgDgBA22) was conducted with 12 genetic markers, and a unique recombinant strain was identified. Detection of T. gondii by CSF cytology, although described in humans, had not been reported previously in dogs, to our knowledge, and was crucial for the diagnosis of toxoplasmosis in the examined dog.

scholarly journals Neospora caninum Infection in a Litter of Labrador Retriever Dogs in Denmark

1995 ◽  
Vol 36 (3) ◽  
pp. 387-391
Author(s):  
A. Flagstad ◽  
Η. Ε. Jensen ◽  
Ι. Bjerkàs ◽  
Κ. Rasmussen
Keyword(s):  
Neospora Caninum ◽  
Labrador Retriever ◽  
Caninum Infection
Sign in / Sign up

Export Citation Format

Share Document