Neospora caninum seroprevalence in dairy cattle in central Thailand

2004 ◽  
Vol 121 (3-4) ◽  
pp. 255-263 ◽  
Author(s):  
T Kyaw ◽  
P Virakul ◽  
M Muangyai ◽  
J Suwimonteerabutr
1999 ◽  
Vol 86 (1) ◽  
pp. 49-57 ◽  
Author(s):  
Pongsatorn Suteeraparp ◽  
Satis Pholpark ◽  
Manvika Pholpark ◽  
Apirom Charoenchai ◽  
Tasanee Chompoochan ◽  
...  

2017 ◽  
Vol 26 (3) ◽  
pp. 292-298 ◽  
Author(s):  
Cesar Augusto Barbosa de Macedo ◽  
Madlaine Frigo Silveira Barbosa de Macedo ◽  
Ana Carolina Miura ◽  
Alessandra Taroda ◽  
Sergio Tosi Cardim ◽  
...  

Abstract The aim of the present study was to investigate the occurrence of N. caninum associated with abortions of dairy cattle from Santa Catarina state, southern Brazil by using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and polymerase chain reaction (PCR). Blood from dairy cows that aborted along with intrathoracic fluid and tissue samples (brain, heart, liver, and lung) from their fetuses were collected and used for serology; PCR, histopathological, and immunohistochemistry (IHC) evaluations were also conducted. Twenty-one cows (51.2%) out of 41, and eight fetuses (26.7%) out of 30 were ELISA (HerdCheck, IDEXX) positive for N. caninum. Dams > 36 months of age had a higher risk of being serum positive than younger animals. PCR and IHC revealed that 38.8% (14/36) and 25.0% (9/36) of the fetuses were positive for N. caninum, respectively for each of the tests. Seropositive cows had a higher frequency of fetuses that were also positive by either intrathoracic fluid, PCR, or IHC. In summary, the present study observed a high frequency of N. caninum in abortions from dairy cows from southern Brazil, with a higher N. caninum prevalence found in cows that were older than 36 months. In addition, serology, PCR, and IHC should be used all together for better diagnosis of neosporosis in cattle.


2006 ◽  
Vol 77 (3-4) ◽  
pp. 254-283 ◽  
Author(s):  
Barbara Häsler ◽  
Katharina D.C. Stärk ◽  
Heinz Sager ◽  
Bruno Gottstein ◽  
Martin Reist

2012 ◽  
Vol 32 (10) ◽  
pp. 1009-1013 ◽  
Author(s):  
Maria E. Sousa ◽  
J.N. Porto Wagnner ◽  
Pedro P.F. Albuquerque ◽  
Orestes L. Souza Neto ◽  
Eduardo B. Faria ◽  
...  

The objective of this study was to investigate the prevalence of anti-Neospora caninum antibodies in cattle from milk producing farms of the microregion of Batalha, state of Alagoas, Brazil, as well as to identify the risk factors associated with the infection. Blood samples were collected from 1,004 cattle of 17 farms for the serological investigation regarding the presence of anti-N. caninum antibodies by the Indirect Immunofluorescence Reaction Technique (IMRT). From the total amount of samples analyzed, 77/1,004 (7.67%) were positive and 927/1,004 (92.33%) were negative. The logistical regression identified that cattle from farms without consortium breeding have an infection risk 6.33 (p<0.001; C.I. 2.89-13.10) times higher than cattle from farms with that type of breeding. Cattle from farms where the aborted fetuses are not adequately buried have an infection risk 3.04 (p<0.001; C.I. 1.64-5.63) times higher than cattle from farms with adequate destination of these fetuses. Infection by N. caninum occurs in cattle of the investigated region. The factors identified in our study can be used as risk indicators, so that control measures could be implemented to avoid infection by N. caninum in the herds of this region.


2002 ◽  
Vol 88 (2) ◽  
pp. 408
Author(s):  
S. L. P. de Souza ◽  
J. S. Guimaraes ◽  
F. Ferreira ◽  
J. P. Dubey ◽  
S. M. Gennari

2009 ◽  
Vol 160 (1-2) ◽  
pp. 51-54 ◽  
Author(s):  
G. Moré ◽  
D. Bacigalupe ◽  
W. Basso ◽  
M. Rambeaud ◽  
F. Beltrame ◽  
...  

1996 ◽  
Vol 9 (5) ◽  
pp. 525-532
Author(s):  
H. Kumagai ◽  
S. Swasdiphanich ◽  
P. Prucsasri ◽  
S. Yimmongkol ◽  
B. Rengsirikul ◽  
...  

2021 ◽  
Author(s):  
Yohannes Equar Messele ◽  
Gebrerufael Girmay ◽  
Bezina Arega Emeru ◽  
Shelema Kelbesa Bora ◽  
Workitu Firomsa Gudeta ◽  
...  

Abstract Background Reproductive problem is one of the main constraints of livestock genetic improvement efforts in tropical countries. The aim of this study was to determine the prevalence of major infectious causes of reproductive problems of dairy cattle in selected dairy farms in central Ethiopia. Overall 86 serum samples were collected from October 2018 to February 2019 from animals with history of reproductive problems. The collected serum was tested for antibody titer against Brucella species, Neospora caninum, Bovine Viral Diarrhea (BVD), Infectious Bovine Rhinotracheitis (IBR) and Q-fever using rose-bengal and enzyme-linked immunosorbent assay (ELISA) tests. Result Among the animals with the history of reproductive disordered; abortion, still birth and repeat breeding cases were found in 61.6%, 19.8% and 18.6%, respectively. The prevalence of IBR, BVD, Neospora caninum and Coxiella brunetti was found to be 79.1%, 38.4%, 3.5% and 1.2%, respectively. The combined infection of both BVD and IBR were detected in 21% of animals. Out of the total animals examined in this study, 95.9% of Jersey breeds were found seropositive to IBR than Boran-Friesian crosses (57.7%). The incidence of BVD was significantly higher in Boran-Friesian crossbred cattle than in Jersey which was found to be 69.3% and 14.3, respectively. The prevalence of IBR and BVD was directly proportional with age of the animal and parity. Conclusion Vaccination against IBR and BVD is not practiced in Ethiopia, the rising level of those diseases in dairy sector needs regular surveillance and control program.


2019 ◽  
Vol 28 (2) ◽  
pp. 215-220 ◽  
Author(s):  
Marcos Enrique Serrano-Martínez ◽  
Cesar Abel Burga Cisterna ◽  
Roberto Carlos Evaristo Romero ◽  
Marco Antonio Quispe Huacho ◽  
Alessandra Matienzo Bermabé ◽  
...  

Abstract Our objective was to identify the direct and indirect presence of Neospora caninum in dairy cattle and their aborted fetuses from Lima, Peru. A total 219 blood samples obtained from dairy cattle with records of spontaneous abortion were collected to detect antibodies against N. caninum in serum with indirect ELISA and search for risk-factor associations. 68 fetal aborted tissue samples of these cows were analyzed by PCR, indirect ELISA and histopathology assay to detect N. caninum presence. The prevalence ratio (PR) and 95% confidence intervals (CI) were estimated. Univariate analysis was performed using the chi-squared test. Among the 68 aborted fetuses collected, 10 (15%) were positive in at least two diagnostic tests. Among 219 serum samples, 46.6% (95% CI: 40.0%-53.3%) were positive. Cows with 4 years or older (PR: 7.10; 95% CI: 4.89-10.67) and multiparous (PR: 1.76; 95% CI: 1.11-2.80) were found to be more likely to possess N. caninum antibodies. This study detects presence of N. caninum in dairy cattle and their aborted fetus from Lima valley, suggesting biosecurity management improve to neosporosis control.


Sign in / Sign up

Export Citation Format

Share Document