scholarly journals Phytotoxic effects of CdTe quantum dots on root meristems of Allium cepa L.

2021 ◽  
Vol 20 (1) ◽  
pp. e890
Author(s):  
Oleksandr Smirnov ◽  
Mariia Kovalenko ◽  
Leila-Аnastasiia Karpets ◽  
Volodymyr Dzhagan ◽  
Olga Kapush ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Allium Cepa ◽  
Proliferative Activity ◽  
Root Meristem ◽  
Plant Cells ◽  
Test System ◽  
Optical Data ◽  
Cytostatic Agents ◽  
Allium Cepa L ◽  
Cadmium Telluride Quantum Dots

The effect of solutions of cadmium telluride quantum dots (CdTe QD) as powerful cytotoxic effectors was investigated using a standard Allium cepa L. test system. The diameters of synthesized CdTe QD derived from the optical data varied within 3 – 4 nm. Toxicity of experimental solutions of CdTe QD at the organism level were evaluated by measuring biomass growth of onion roots and cytotoxic influence was estimated based on proliferative activity of root meristem cells. All of CdTe QD experimental solutions in concentration 10 µM significantly inhibited the growth of Allium cepa L. roots, proliferative activity, and total dehydrogenase activity. Relation between the QDs size and their phytotoxicity was not found. However, the highest inhibiting impact was for QDs solution with a nanocrystals size of 3.5 nm. Their ability to penetrate into cells and interact with their intracellular components may cause inhibiting mitosis without fixed clastogenic and aneugenic effects. Solutions of CdTe QD at a given concentration can be considered as potent cytostatic agents for plant cells with antimitotic properties.

scholarly journals Phytotoxicity of colloidal solutions of stabilized and non-stabilized nanoparticles of essential metals and their oxides

2019 ◽  
Vol 18 (1) ◽  
pp. 1-9
Author(s):  
Yevheniia Konotop ◽  
Kseniia Stepanchenko ◽  
Leila-Anastasiia Karpets ◽  
Andrii Zinchenko ◽  
Mariia Kovalenko ◽  
...  
Keyword(s):  
Metal Nanoparticles ◽  
Allium Cepa ◽  
Proliferative Activity ◽  
Growth Response ◽  
Root Meristem ◽  
Test System ◽  
Standard Test ◽  
Essential Metals ◽  
Colloidal Solutions ◽  
Integral Index

Abstract Advances in nanotechnology in various fields of human activity contribute to increase of their production, improved properties and wider implementation of nanomaterials. However, increasing use may enhance their release into the environment and can lead to affecting human health. The toxicity of colloidal solutions of metal nanoparticles (Cu, Mn) and their oxides, obtained in the absence and presence of a stabilizer, was examined and compared with the use of the standard test system of Allium cepa L.. The phytotoxicity of the experimental solutions was evaluated according to the growth response of the onion roots; the cyto- and genotoxicity were estimated due to the proliferative activity of the root meristem cells. It was established that solutions of stabilized metal nanoparticles were at given concentration toxic to Allium cepa L. according to the integral index of roots growth, however, were not cytotoxic. Difference in the phytotoxicity of stabilized and non-stabilized metal nanoparticles and their oxides depended on their phase composition and affected root growth.

scholarly journals Action of Aqueous Extracts of Phyllanthus niruri L. (Euphorbiaceae) leaves on Meristematic Root Cells of Allium cepa L.

2014 ◽  
Vol 86 (3) ◽  
pp. 1131-1137 ◽  
Author(s):  
ERASMOVLANE S.B. NEVES ◽  
PAULO MICHEL PINHEIRO FERREIRA ◽  
LEONARDO H.G.M. LIMA ◽  
ANA PAULA PERON
Keyword(s):  
Cell Cycle ◽  
Allium Cepa ◽  
Root Meristem ◽  
Test System ◽  
Aqueous Extracts ◽  
Phyllanthus Niruri ◽  
Root Cells ◽  
Allium Cepa L ◽  
Chi Squared ◽  
Meristematic Root

This study aimed to evaluate the effects of aqueous extracts of dried Phyllanthus niruri L. (stonebreaker) leaves on Allium cepa L. root meristem cells at four concentrations, 0.02 (usual concentration), 0.04, 0.06 and 0.08mg/mL and exposure times of 24 and 48 hours. For each concentration we used a group of five onion bulbs that were first embedded in distilled water and then transferred to their respective concentrations. The radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were prepared by the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5000 for each control and exposure time. The calculated mitotic indices were subjected to the Chi-squared statistical analysis (p<0.05). From the results obtained it was observed that all four concentrations tested had significant antiproliferative effect on the cell cycle of this test system. We also found the presence of cellular aberrations such as colchicined metaphases, anaphasic and telophasic bridges, and micronuclei in the two exposure times for all concentrations evaluated. Therefore, under the conditions studied the concentrations of aqueous extracts of leaves of P. niruri showed to be cytotoxic and genotoxic.

scholarly journals Genotoxicity of tetrodotoxin from puffer fish tested in root meristem cells of Allium cepa L.

Mutagenesis ◽  
1997 ◽  
Vol 12 (4) ◽  
pp. 265-269 ◽  
Author(s):  
Samanta S. Khora ◽  
Kamal K. Panda ◽  
Brahma B. Panda
Keyword(s):  
Allium Cepa ◽  
Root Meristem ◽  
Allium Cepa L ◽  
Puffer Fish

Short-term assessment of cadmium toxicity and uptake from different types of Cd-based Quantum Dots in the model plant Allium cepa L.

2018 ◽  
Vol 153 ◽  
pp. 23-31 ◽  
Author(s):  
Pavlína Modlitbová ◽  
Pavel Pořízka ◽  
Karel Novotný ◽  
Jana Drbohlavová ◽  
Ivana Chamradová ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Allium Cepa ◽  
Cadmium Toxicity ◽  
Short Term ◽  
Model Plant ◽  
Allium Cepa L ◽  
Different Types

scholarly journals Effect of triazophos on mitotic activity and chromosomal behavior in root meristems of Allium cepa L.

2010 ◽  
Vol 6 ◽  
pp. 4-7 ◽  
Author(s):  
Archana Pandey ◽  
Shyam R Sakya
Keyword(s):  
Mitotic Activity ◽  
Allium Cepa ◽  
Root Meristem ◽  
Plant Science ◽  
Root Tip ◽  
Allium Cepa L ◽  
Meristematic Region ◽  
Cellular Abnormalities ◽  
Tip Cells ◽  
Root Tip Cells

Effect of triazophos (an organophosphorous insecticide) on mitotic activity and chromosomal behavior in the meristematic region of root tip cells of Allium cepa L. was assessed. The insecticide showed mitotic depression and positive chromo-toxic effects. Abnormalities, such as stickiness, plasmolysed cells, equatorial plate shifting, polar shifting, irregular chromosome arrangement, precocious arms formation, bridge formation, C-metaphase, fragmentation of chromosomes, unequal cytokinesis, diagonal cytokinesis, delayed cytokinesis and formation of binucleated cells, were recorded in the chemically pretreated root meristem. Key-words: chromosomal and cellular abnormalities; cytotoxic effect; mitotic index; phase indices.DOI: 10.3126/botor.v6i0.2903 Botanica Orientalis - Journal of Plant Science (2009) 6: 4-7

scholarly journals Investigation of cytotoxic and mutagenic effects of Malpighia glabra L. (barbados cherry) fruit pulp and vitamin C on plant and animal test systems

2012 ◽  
Vol 32 (2) ◽  
pp. 405-411 ◽  
Author(s):  
Elisângela Düsman ◽  
Márcia Flores da Silva Ferreira ◽  
Alessandra Paim Berti ◽  
Rosinete Gonçalves Mariucci ◽  
Mário Sérgio Mantovani ◽  
...  
Keyword(s):  
Cell Division ◽  
Vitamin C ◽  
Allium Cepa ◽  
Wistar Rats ◽  
Chromosomal Abnormalities ◽  
Bone Marrow Cells ◽  
Recovery Period ◽  
Test System ◽  
Liquid Form ◽  
Allium Cepa L

Fruits are important sources of nutrients in human diet, and Barbados Cherry (Malpighia glabra L.) is of particular interest due to its high content of antioxidants. Diets rich in fruits and vegetables protect individuals against diseases and cancer, but excessive intake of vitamins may act as pro-oxidant and generate changes in DNA. To evaluate the effect of different in natura (BAN) and frozen (BAF) Barbados Cherry pulp concentrations and synthetic vitamin C in liquid form (VC) on the chromosome level and the cell cycle division, root meristeme cells of Allium cepa L. and bone marrow cells of Wistar rats Rattus norvegicus, were used as test system. In Allium cepa L., BAN, at the highest concentration (0.4 mg.mL-1) and BAF, at the lowest concentration (0.2 mg.mL-1), inhibited cell division, and there was recovery of cell division after the recovery period in water only for BAN. In the Wistar rats, all treatments with Barbados Cherry, either acute or subchronic, were not cytotoxic or mutagenic; only the highest concentration of VC increased significantly the rate of chromosomal abnormalities. The data obtained are important to reinforce the use of Barbados Cherry fruit in the diet.

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