scholarly journals Protozoan genital invasions caused by the representatives of trichomonas and giardia

2020 ◽  
Vol 73 (2) ◽  
pp. 380-383
Author(s):  
Pavlo V. Fedorych ◽  
Gennadiy I. Mavrov ◽  
Tetiana V. Osinska ◽  
Yuliia V. Shcherbakova
Keyword(s):  
Systematic Review ◽  
Polymerase Chain Reaction ◽  
Giardia Lamblia ◽  
Trichomonas Vaginalis ◽  
Pathogenic Microorganisms ◽  
Chain Reaction ◽  
Pilot Studies ◽  
Polymerase Chain ◽  
Pentatrichomonas Hominis ◽  
Genitourinary Infections

The aim was to perform systematic review of genitourinary protozoan invasion and analyze their pathogenicity and the ability to influence the genitourinary infections. Materials and methods: For systematic review of papers the EMBASE and PubMed databases were searched. We also reviewed our own pilot studies using real-time polymerase chain reaction (PCR) to determine Trichomonas tenax, Pentatrichomonas hominis and Giardia lamblia. Conclusions: Trichomonas tenax, Pentatrichomonas hominis, Giardia lamblia can cause genitourinary invasion in addition to Trichomonas vaginalis. Their eradication is obligatory at least for not keeping intact pathogenic microorganisms phagocyted by Trichomonas spp. Defining the protozoan forms is important in preventing of genital infections recurrences and reinfections.

scholarly journals THE ROLE OF PROTOZOAL INFESTATIONS IN CHRONIC INFLAMMATION EXACERBATIONS IN PATIENTS WITH GENITOURINARY PATHOLOGY

2018 ◽  
Vol 5 ◽  
pp. 28-33 ◽  
Author(s):  
Pavlo Fedorych ◽  
Gennadiy Mavrov
Keyword(s):  
Polymerase Chain Reaction ◽  
Chronic Inflammation ◽  
Giardia Lamblia ◽  
Trichomonas Vaginalis ◽  
Inflammatory Diseases ◽  
Chain Reaction ◽  
Genitourinary System ◽  
Polymerase Chain ◽  
High Level

The aim: to study the prevalence of protozoal infestations in cases of acute inflammatory exacerbations in genitourinary clinic. Materials and methods. The method of polymerase chain reaction was used to examine 158 subjects with chronic inflammation of the genitourinary system exacerbations. Results. Infestation of the genitourinary system was identified in 72 patients (45.6 %). Trichomonas infestation was identified in 63 (87.5 %) of them. Trichomonas vaginalis was identified in 1 (1.4 %) subject. Other Trichomonas species – in 62 (86.1 %) subjects. 12 (16.7 %) had Trichomonas tenax, and 50 (69.4 %) – Pentatrichomonas hominis. Giardia lamblia was identified in 9 patients – i.e. in 12.5 % individuals with infestation of the genitourinary system, or in 5.7 % among subjects examined for STIs in this study. Conclusions: High level of Trichomonas infestation of the genitourinary system was identified in subjects with of chronic inflammatory exacerbations of the genitourinary system. In most cases, infestations were caused by Trichomonas species other than Trichomonas vaginalis, as well as by Giardia lamblia. An assumption about a certain role of these pathogens in the onset or further course of inflammatory diseases of the genitourinary system was made.

scholarly journals Polymerase Chain Reaction (PCR) as a Potential Point of Care Laboratory Test for Leprosy Diagnosis—A Systematic Review

2018 ◽  
Vol 3 (4) ◽  
pp. 107 ◽  
Author(s):  
Sushma Tatipally ◽  
Aparna Srikantam ◽  
Sanjay Kasetty
Keyword(s):  
Systematic Review ◽  
Polymerase Chain Reaction ◽  
Point Of Care ◽  
Effective Control ◽  
Chain Reaction ◽  
Review Analysis ◽  
Pubmed Database ◽  
Early Case ◽  
Polymerase Chain ◽  
Clinical Conditions

Leprosy is an infectious disease caused by Mycobacterium leprae and mainly affects skin, peripheral nerves, and eyes. Suitable tools for providing bacteriological evidence of leprosy are needed for early case detection and appropriate therapeutic management. Ideally these tools are applicable at all health care levels for the effective control of leprosy. This paper presents a systematic review analysis in order to investigate the performance of polymerase chain reaction (PCR) vis-à-vis slit skin smears (SSS) in various clinical settings and its potential usefulness as a routine lab test for leprosy diagnosis. Records of published journal articles were identified through PubMed database search. Twenty-seven articles were included for the analysis. The evidence from this review analysis suggests that PCR on skin biopsy is the ideal diagnostic test. Nevertheless, PCR on SSS samples also seems to be useful with its practical value for application, even at primary care levels. The review findings also indicated the necessity for improving the sensitivity of PCR and further research on specificity in ruling out other clinical conditions that may mimic leprosy. The M. leprae-specific repetitive element (RLEP) was the most frequently-used marker although its variable performance across the clinical sites and samples are a matter of concern. Undertaking further research studies with large sample numbers and uniform protocols studied simultaneously across multiple clinical sites is recommended to address these issues.

scholarly journals Diagnosis of Trichomonas vaginalis using real-time polymerase chain reaction (PCR) among women at Institut Pasteur of Cte dIvoire

2018 ◽  
Vol 12 (43) ◽  
pp. 988-993
Author(s):  
T. Blavo-Kouamé ◽  
K. E. Angora ◽  
A. Yéo ◽  
A. Ouattara ◽  
A. Ira-Bonouman ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Trichomonas Vaginalis ◽  
Chain Reaction ◽  
Polymerase Chain
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