scholarly journals Rescue and genetic assessment of soybean-nodulating Bradyrhizobium strains from an experimental field thirty years after inoculation

2020 ◽  
Vol 26 (1) ◽  
pp. 173-189
Author(s):  
Adriana Giongo ◽  
Adriana Ambrosini ◽  
João Ruy Jardim Freire ◽  
Luciano Kayser ◽  
Maria Helena Bodanese-Zanettin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Nitrogen Fixation ◽  
Genetic Stability ◽  
Soybean Variety ◽  
Host Genotype ◽  
High Genetic Diversity ◽  
Experimental Field ◽  
Genetic Assessment

As the long-term phenotype and genetic stability of bacteria used as inoculant are important parameters in their ecology and for agricultural purposes, this study aimed genotypically characterize several-rescued bradyrhizobia of an experimental field thirty years after the first inoculation. A high genetic diversity of 30 bradyrhizobia isolates was observed, either by AFLP (H = 4.87) or rep-PCR (H = 4.18). The results indicate that the Bradyrhizobium population that persists in the Eldorado soil is genetically very diverse and different from the parental strains. All isolates were infective and trapped in IAS-5 soybean variety maintaining their nodulation and nitrogen fixation properties. Given that many rhizobia in a soil can lost the infective capacity and that the host genotype can affect the spectrum of rhizobial genotype selected from a soil, the genetic diversity of the complete bradyrhizobia population in Eldorado soil could be even higher than the identified in this work.

scholarly journals Variability of Major Phenyletanes and Phenylpropanoids in 16-Year-Old Rhodiola rosea L. Clones in Norway

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3463 ◽  
Author(s):  
Abdelhameed Elameen ◽  
Vera M. Kosman ◽  
Mette Thomsen ◽  
Olga N. Pozharitskaya ◽  
Alexander N. Shikov
Keyword(s):  
Genetic Diversity ◽  
Bioactive Compounds ◽  
Freeze Drying ◽  
Germplasm Collection ◽  
Rhodiola Rosea ◽  
Cinnamyl Alcohol ◽  
Drying Method ◽  
High Genetic Diversity ◽  
Conventional Drying

Rhodiola rosea L. (roseroot) is an adaptogen plant belonging to the Crassulaceae family. The broad spectrum of biological activity of R. rosea is attributed to its major phenyletanes and phenylpropanoids: rosavin, salidroside, rosin, cinnamyl alcohol, and tyrosol. In this study, we compared the content of phenyletanes and phenylpropanoids in rhizomes of R. rosea from the Norwegian germplasm collection collected in 2004 and in 2017. In general, the content of these bioactive compounds in 2017 was significantly higher than that observed in 2004. The freeze-drying method increased the concentration of all phenyletanes and phenylpropanoids in rhizomes compared with conventional drying at 70 °C. As far as we know, the content of salidroside (51.0 mg g−1) observed in this study is the highest ever detected in Rhodiola spp. Long-term vegetative propagation and high genetic diversity of R. rosea together with the freeze-drying method may have led to the high content of the bioactive compounds observed in the current study.

Long-term isolation and stability explain high genetic diversity in the Eastern Himalaya

2014 ◽  
Vol 23 (3) ◽  
pp. 705-720 ◽  
Author(s):  
Yanhua Qu ◽  
Per G. P. Ericson ◽  
Qing Quan ◽  
Gang Song ◽  
Ruiying Zhang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Eastern Himalaya ◽  
High Genetic Diversity

scholarly journals Cellar-Associated Saccharomyces cerevisiae Population Structure Revealed High-Level Diversity and Perennial Persistence at Sauternes Wine Estates

2016 ◽  
Vol 82 (10) ◽  
pp. 2909-2918 ◽  
Author(s):  
Marine Börlin ◽  
Pauline Venet ◽  
Olivier Claisse ◽  
Franck Salin ◽  
Jean-Luc Legras ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Diversity ◽  
Population Analysis ◽  
White Wine ◽  
High Genetic Diversity ◽  
Spontaneous Fermentation ◽  
Content Type ◽  
Temporal Succession ◽  
High Level

ABSTRACTThree wine estates (designated A, B, and C) were sampled in Sauternes, a typical appellation of the Bordeaux wine area producing sweet white wine. From those wine estates, 551 yeast strains were collected between 2012 and 2014, added to 102 older strains from 1992 to 2011 from wine estate C. All the strains were analyzed through 15 microsatellite markers, resulting in 503 uniqueSaccharomyces cerevisiaegenotypes, revealing high genetic diversity and a low presence of commercial yeast starters. Population analysis performed usingFstgenetic distance or ancestry profiles revealed that the two closest wine estates, B and C, which have juxtaposed vineyard plots and common seasonal staff, share more related isolates with each other than with wine estate A, indicating exchange between estates. The characterization of isolates collected 23 years ago at wine estate C in relation to recent isolates obtained at wine estate B revealed the long-term persistence of isolates. Last, during the 2014 harvest period, a temporal succession of ancestral subpopulations related to the different batches associated with the selective picking of noble rotted grapes was highlighted.IMPORTANCEHigh genetic diversity ofS. cerevisiaeisolates from spontaneous fermentation on wine estates in the Sauternes appellation of Bordeaux was revealed. Only 7% of all Sauternes strains were considered genetically related to specific commercial strains. The long-term persistence (over 20 years) ofS. cerevisiaeprofiles on a given wine estate is highlighted.

scholarly journals Subtype Characterization and Zoonotic Potential of Cryptosporidium felis in Cats in Guangdong and Shanghai, China

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 89
Author(s):  
Jiayu Li ◽  
Fuxian Yang ◽  
Ruobing Liang ◽  
Sheng Guo ◽  
Yaqiong Guo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Characterization ◽  
Zoonotic Potential ◽  
Common Occurrence ◽  
High Genetic Diversity ◽  
Gp60 Gene ◽  
The Common ◽  
Subtyping Tool

Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.

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