scholarly journals Effect of POU1F1 Gene Haplotypes on Eights and Milk Production of Awassi Sheep

2019 ◽  
Vol 32 (2) ◽  
pp. 85-94
Author(s):  
Azhar A. Jaffar ◽  
Amad F. Hassan ◽  
Waleed Y. Kassim
Keyword(s):  
Statistical Analysis ◽  
Gel Electrophoresis ◽  
Pcr Amplification ◽  
Gene Bank ◽  
College Of Agriculture ◽  
Pou1f1 Gene ◽  
Nitrogen Bases ◽  
Awassi Sheep ◽  
Amplification Process ◽  
Productive Traits

The aim of the study was to detect polymorphism in the POU1F1 gene in Iraqi Awassi sheep breed, as well as to establish if haplotype of POU1F1 gene could be associated with productive traits. This study was carried out at Al-Kafeel station, Karbala city during the period of 01/10/2017 until 01/08/2018. The study included 46 Awassi ewes with their 52 lambs. The laboratory analyses were conducted at the Laboratory of Molecular Genetics, College of Agriculture, University of Basrah. Results showed the successfulness of the PCR amplification process for all six examined fragments of the POU1F1 gene. Gel electrophoresis was conducted using agarose 2%, the product sizes were 637bp, 789bp, 999bp, 868bp, 1190 bp, and 469bp for the fragments P1, P2, P3, P4, P5, and P6 respectively. The analysis of the nitrogen bases sequences of the POU1F1 gene for the studied fragments showed a change in 12 different sites of the gene. These changes resulted in 7 haplotypes of H1-H7. The results showed significant influence (P<0.05) in haplotypes of the POU1F1 gene on birth weight . However, haplotypes showed no significant effect on lambs’ weights at weaning, six months and daily weight gains during all periods. Statistical analysis showed that different haplotypes of the POU1F1 gene did not influence on milk productive traits. The examined fragments of the POU1F1 Awassi gene have been submitted to Gene Bank under the accession numbers (LC469323 to LC469349).

scholarly journals Effect of IGF-1 and GH Genes Polymorphism on Weights and Body Measurements of Awassi Lambs in Different Ages

2019 ◽  
Vol 32 (1) ◽  
pp. 39-46
Author(s):  
Rahman H. Al Qasimi ◽  
Amad F. Hassan ◽  
Bassam Y. Khudair
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Genetic Analysis ◽  
Body Measurements ◽  
College Of Agriculture ◽  
Awassi Sheep ◽  
Superior Genotype ◽  
Weaning Age ◽  
Productive Traits ◽  
Awassi Lambs

The study was carried on 68 ewes of local Awassi sheep in the Al-Kafeel sheep station Karbala governorate, Iraq for the period from 1/10/2017 to 1/8/2018. Genetic analysis was carried out in the molecular genetics laboratory at the College of Agriculture / Basrah University in order to extract (DNA) and determine the genotype  of the IGF-1(Insulin-like growth factor 1) and GH (Growth Hormone) genes. This study aimed to know the association between the genotype of the ewes of IGF-1 and GH  genes and their relationship to the productive traits of the lambs, which included the weights of lambs from birth to weaning and body measurements, Three of the  genotypes for IGF-1 were revealed AG, GC, CC and two for GH CT, TC . The effect of IGF-1 genotypes was significant (P<0.05) on weights of lambs at weaning, age  6 months and some body measurements with superior genotype GC. The genotypes of  GH had a significant effect on the weight of the lambs at birth by CT superiority. While the weights of weaning, six months and body measurements were not significantly affected by different genotypes.

scholarly journals PSIV-2 Investigating candidate scur genes in Bos taurus breeds

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 226-227 ◽  
Author(s):  
Crystal Ketel ◽  
Mika Asai-Coakwell
Keyword(s):  
Candidate Genes ◽  
Gel Electrophoresis ◽  
Bos Taurus ◽  
Pcr Amplification ◽  
Genetic Mutation ◽  
Mapping Data ◽  
Future Studies ◽  
Feedlot Steers ◽  
Pcr Rflp ◽  
Beef Producers

Abstract Scurs (loose horns) are inherited in a sex-influenced manner and are believed to appear in cattle when the animal is heterozygous (Pp) for the polled mutation. They are unwanted by beef producers, but are difficult to eradicate because scurs are epistatic to the polled mutation. With the development of a test for the 202 bp indel on BTA1 resulting in the polled phenotype in Celtic breeds, horned (pp) and polled (PP/Pp) animals can be genotyped at this locus. The aims of this study were: 1) to confirm the polled genotype in scurred families from a Canadian beef research herd (SCBRH), scurred cattle families from producers (SCFP), and polled and scurred feedlot steers using the Celtic poll test (PC), and 2) to identify new candidate genes between the recombinant genes of the postulated scur loci on BTA19. Through PCR amplification, the polled/horned genotype was confirmed in the SCBRH, SPCF, and 153 phenotyped feedlot steers with gel electrophoresis. One family from the SPCF, 26 scurred and 10 polled feedlot steers were genotyped as horned. Removing the SPCF horned animals from the scur loci mapping data changed the recombinant markers and created a new boundary, resulting in examination of five new candidate genes (CTDNEP1, FGF11, SOX15, SHBG, DHRS7C) based on function and position. To identify SNPs segregating with scurs, 16 animals were chosen from the PC genotyped feedlot steers, 8 Pp scurred steers and 8 Pp polled steers. Two SNP’s found in CTDNEP1 and DHRS7C were examined in the SCBRH with PCR-RFLP using BseRI and AciI, respectively, but did not segregate with scurs. In conclusion, careful phenotyping and genotyping for the polled/horned status of an animal should be confirmed for future studies to determine the genetic mutation resulting in scurs.

scholarly journals Comparison of methods for detection of four common nosocomial pathogens on hospital textiles

2014 ◽  
Vol 53 (1) ◽  
pp. 17-25 ◽  
Author(s):  
Sabina Fijan ◽  
Sonja Šostar Turk ◽  
Urška Rozman
Keyword(s):  
Gel Electrophoresis ◽  
Pcr Amplification ◽  
Agarose Gel Electrophoresis ◽  
Bacterial Concentration ◽  
Plate Method ◽  
Identification Process ◽  
Hospital Textiles ◽  
Non Destructive ◽  
Phenotype Identification

AbstractIntroduction: Although the most common vehicle for transmission of health-care acquired infections is the personto- person transmission route, the role of environment should not be ignored and hospital linen may contribute to the spreading of nosocomial infections. The contact plate method and swabbing are common methods for sampling microorganisms on textiles; however, results are available after two days as they are based on incubation followed by phenotype identification. An important alternative is using quick wash-off methods followed by PCR detection, which shortens the identification process from two days to a few hours.Methods:The following test microorganisms at different concentrations were inoculated onto textile swatches and dried overnight: Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Clostridium difficile. RODAC plate sampling as well as a non-destructive wash-off method for capturing microorganisms from the textiles using a Morapex device were used. The elution suspension from the Morapex device was used for two methods. In the first method, classical incubation on selective media followed by phenotypic identification was used and in the second method DNA was extracted from the elution suspension followed by amplification and agarose gel electrophoresis to visualize amplified products.Conclusions:All chosen bacteria were found using all methods. However, the most sensitive proved to be detection using PCR amplification as we detected the sample with initial concentration of 102 cfu/mL inoculated onto the textile surface before drying. The final detectable recovered bacterial concentration on textiles was up to 10 cfu/mL.

Processing of Data Generated by 2-Dimensional Gel Electrophoresis for Statistical Analysis:  Missing Data, Normalization, and Statistics

2004 ◽  
Vol 3 (6) ◽  
pp. 1210-1218 ◽  
Author(s):  
Jinsook Chang ◽  
Holly Van Remmen ◽  
Walter F. Ward ◽  
Fred E. Regnier ◽  
Arlan Richardson ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Missing Data ◽  
Gel Electrophoresis ◽  
Data Normalization

Determination of incompatibility (S) genotypes of sweet cherries in the Hungarian gene-bank by a PCR-based method

2010 ◽  
Vol 58 (4) ◽  
pp. 377-384 ◽  
Author(s):  
Z. Békefi ◽  
S. Vaughan ◽  
K. Tobutt
Keyword(s):  
Central Region ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Pcr Amplification ◽  
Gene Bank ◽  
The Novel ◽  
Incompatibility Group ◽  
Sweet Cherries ◽  
Intron Region

The sweet cherry (Prunus avium L.) gene-bank collection in Hungary comprises mainly local cultivars. The incompatibility (S) genotypes of 48 accessions from the central region of Hungary were investigated by PCR amplification of the intron regions of the SRNase and SFB genes responsible for compatibility relationships in sweet cherry. The Sgenotypes of 38 accessions were completely determined; they showed various pairs of nine alleles and could be assigned to 15 of the existing incompatibility groups or, in the case of three accessions having the novel genotype S6S13, to the new incompatibility group XLII. For 10 accessions only one S-allele could be identified, as a single S-RNase product was generated and the intron region of the SFB gene of the second allele could not be amplified.

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