Expression of a Vacuolar Na+/H+ Antiporter Gene of Alfalfa Enhances Salinity Tolerance in Transgenic Arabidopsis

2008 ◽  
Vol 34 (4) ◽  
pp. 557-564 ◽  
Author(s):  
Bao-Yan AN
Keyword(s):  
2013 ◽  
Vol 19 (2) ◽  
pp. 57-65
Author(s):  
MH Kabir ◽  
MM Islam ◽  
SN Begum ◽  
AC Manidas

A cross was made between high yielding salt susceptible BINA variety (Binadhan-5) with salt tolerant rice landrace (Harkuch) to identify salt tolerant rice lines. Thirty six F3 rice lines of Binadhan-5 x Harkuch were tested for salinity tolerance at the seedling stage in hydroponic system using nutrient solution. In F3 population, six lines were found as salt tolerant and 10 lines were moderately tolerant based on phenotypic screening at the seedling stage. Twelve SSR markers were used for parental survey and among them three polymorphic SSR markers viz., OSR34, RM443 and RM169 were selected to evaluate 26 F3 rice lines for salt tolerance. With respect to marker OSR34, 15 lines were identified as salt tolerant, 9 lines were susceptible and 2 lines were heterozygous. While RM443 identified 3 tolerant, 14 susceptible and 9 heterozygous rice lines. Eight tolerant, 11 susceptible and 7 heterozygous lines were identified with the marker RM169. Thus the tested markers could be efficiently used for tagging salt tolerant genes in marker-assisted breeding programme.DOI: http://dx.doi.org/10.3329/pa.v19i2.16929 Progress. Agric. 19(2): 57 - 65, 2008


2018 ◽  
Vol 51 (3) ◽  
pp. 51-68 ◽  
Author(s):  
M.K. Hasan ◽  
M.S. Islam ◽  
M.R. Islam ◽  
H.N. Ismaan ◽  
A. El Sabagh

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.


2016 ◽  
Vol 3 (1) ◽  
Author(s):  
ANSHUMAN SINGH ◽  
ASHWANI KUMAR ◽  
R.K. YADAV ◽  
ASHIM DUTTA ◽  
D.K. SHARMA

Guav a cv . Allahabad Safeda w as grown in saline soils and irrigated with the best av ailable w ater -1 -1 + -1 (EC 2.8 dS m ). Based on chemical composition (pH- 7.1, EC - 2.8 dS m , Na - 20.04 meq l and IW IW sodium adsorption ratio- 4.86), irrigation w ater w as categorized as marginally saline. The soil pH 2 -1 w as mostly below 8.5 but mean electrical conductivity (EC ) v alues ranged from 0.5-2 dS m 2 indicating moderate to high salinity in the experimental soil. After one-y ear of experimentation, fiv e plants randomly selected from each treatment and the data w ere recorded. Plant height -1 -1 significantly increased (LSD 5%) with increase in salinity from 0.5 dS m to 1.4 dS m . A similar -1 trend w as noted with respect to stem girth. The av erage plant height at 0.5, 0.9 and 1.4 dS m salinity lev els w as 98.3 cm, 108.3 cm and 123 cm, respectiv ely whereas the corresponding stem girth v alues -1 w ere 2.24 cm, 2.28 cm and 2.46 cm. At 2 dS m salinity ,how ev er , both av erage plant height (94.6 cm) and stem girth (2.24 cm) significantly decreased and w ere found to be comparable to control (0.5 dS -1 + -1 m ) v alues. Plants show ed negligible Na accumulation in leav es up to 1.4 dS m salinity , but -1 + exposure to elev ated salinity (2 dS m ) significantly increased leaf Na (0.16% DW). These data -1 indicated a salinity tolerance (EC )threshold of about 1.5 dS m inguav a cultiv ar Allahabad Safeda.


2019 ◽  
Vol 55 (No. 2) ◽  
pp. 61-69 ◽  
Author(s):  
Dorsaf Allel ◽  
Anis BenAmar ◽  
Mounawer Badri ◽  
Chedly Abdelly

Soil salinity is one of the main factors limiting cereal productivity in worldwide agriculture. Exploitation of natural variation in local barley germplasm is an effective approach to overcome yield losses. Three gene pools of North African Hordeum vulgare L. grown in Tunisia, Algeria and Egypt were evaluated at the reproductive stage under control and saline conditions. Assessment of stress tolerance was monitored using morphological, yield-related traits and phenological parameters of reproductive organs showing significant genetic variation. High heritability and positive relationships were found suggesting that some traits associated with salt tolerance could be used as selection criteria. The phenotypic correlations revealed that vegetative traits including shoot biomass, tiller number and leaf number along with yield-related traits such as spike number, one spike dry weight, grain number/plant and grain number/spike were highly positively correlated with grain yield under saline conditions. Hence, these traits can be used as reliable selection criteria to improve barley grain yield. Keeping a higher shoot biomass and longer heading and maturity periods as well as privileged filling ability might contribute to higher grain production in barley and thus could be potential target traits in barley crop breeding toward improvement of salinity tolerance. Multiple selection indices revealed that salt tolerance trait index provided a better discrimination of barley landraces allowing selection of highly salt-tolerant and highly productive genotypes under severe salinity level. Effective evaluation of salt tolerance requires an integration of selection indices to successfully identify and characterize salt tolerant lines required for valuable exploitation in the management of salt-affected areas.  


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