scholarly journals Molecular identification and breeding application of allelic variation of grain weight gene in wheat from the Yellow-Huai-River Valley

2021 ◽  
Vol 47 (11) ◽  
pp. 2091-2098
Author(s):  
Fu-Yan ZHANG ◽  
Zhong-Jie CHENG ◽  
Xiao-Jie CHEN ◽  
Jia-Huan WANG ◽  
Feng CHEN ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Allelic Variation ◽  
River Valley ◽  
Grain Weight ◽  
Huai River ◽  
Breeding Application

scholarly journals Cloning, characterization of TaGS3 gene and identification of allelic variation associated with kernel traits in wheat (Triticum aestivum L.)

2019 ◽  
Author(s):  
Jian Yang ◽  
Yanjie Zhou ◽  
Yu'e Zhang ◽  
Weiguo Hu ◽  
Qiuhong Wu ◽  
...  
Keyword(s):  
Allelic Variation ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Allelic Frequency ◽  
Yield Component ◽  
Grain Weight ◽  
Specific Gene ◽  
Wheat Production ◽  
Trait Locus ◽  
Production Areas

Abstract Background: Grain weight is an important yield component. Selection of advanced lines with heavy grains show high grain sink potentials and strong sink activity, which is an increasingly important objective in wheat breeding programs. Rice OsGS3 has been identified as a major quantitative trait locus for both grain weight and grain size. However, allelic variation of GS3 has not been characterized previously in hexaploid wheat. Results : We cloned 2445, 2393, and 2409 bp sequences of the homologs TaGS3-4A , TaGS3-7A , and TaGS3-7D in wheat ‘Changzhi 6406’, a cultivar that shows high grain weight. The TaGS3 genes each contained five exons and four introns, and encoded a deduced protein of 170, 169, and 169 amino acids, respectively. Phylogenetic analysis of plant GS3 protein sequences revealed GS3 to be a monocotyledon-specific gene and the GS3 proteins were resolved into three classes. The length of the atypical Gγ domain and the cysteine-rich region was conserved within each class and not conserved between classes. A single-nucleotide polymorphism in the fifth exon (at position 1907) of TaGS3-7A leads to an amino acid change (ALA/THR) and showed different frequencies in two pools of Chinese wheat accessions representing extremes in grain weight. Association analysis indicated that the TaGS3-7A-A allele was associated with higher grain weight in the natural population. The TaGS3-7A-A allele was favoured in global modern wheat cultivars but the allelic frequency varied among different wheat-production regions of China, which indicated that this allele is of potential utility to improve wheat grain weight in certain wheat-production areas of China. Conclusions : The novel molecular information on wheat GS3 homologs and the KASP functional marker designed in this study may be useful in marker-assisted breeding for genetic improvement of wheat.

scholarly journals Cloning, characterization of TaGS3 gene and identification of allelic variation associated with kernel traits in wheat (Triticum aestivum L.)

2019 ◽  
Author(s):  
Jian Yang ◽  
Yanjie Zhou ◽  
Yu'e Zhang ◽  
Weiguo Hu ◽  
Qiuhong Wu ◽  
...  
Keyword(s):  
Allelic Variation ◽  
Triticum Aestivum L ◽  
Yield Component ◽  
Grain Weight ◽  
Specific Gene ◽  
Rich Region ◽  
Component Selection ◽  
Important Objective ◽  
Selection Of

Abstract Background Grain weight is an important yield component. Selection of heavy grain wheat showed big grain sink-potentials and strong sink activity, which was becoming an important objective during the breeding program. Rice OsGS3 gene has been identified as a major QTL for both grain weight and grain size. However, allelic variation of GS3 gene has not been characterized in wheat. Results Here we isolated 2445bp, 2393bp and 2409bp genomic sequences of TaGS3-4A, TaGS3-7A and TaGS3-7D genes in Changzhi 6406 with high grain weight value by homologous cloning. All TaGS3 gene showed five exon and four intron and encoding putative 170, 169 and 169 amino acids. Phylogenetic analysis revealed that GS3 was a monocot-specific gene and all GS3 protein were split into three classes. The length of the atypical Gγ domain and the cysteine-rich region was conserved within class and nonconserved inter-class. An SNP at the fifth exon (position of 1907) of TaGS3-7A gene would lead to amino acid change (ALA/THR) and showed different frequency in two pools with different grain weight. Further association analysis indicated that TaGS3-7A-A allele was associated with higher grain weight in the natural population. Conclusions These results providing some useful variants and a functional marker for further marker assistant breeding.

Phytoliths reveal the earliest interplay of rice and broomcorn millet at the site of Shuangdun (ca. 7.3–6.8 ka BP) in the middle Huai River valley, China

2019 ◽  
Vol 102 ◽  
pp. 26-34 ◽  
Author(s):  
Wuhong Luo ◽  
Chunguang Gu ◽  
Yuzhang Yang ◽  
Dong Zhang ◽  
Zhonghe Liang ◽  
...  
Keyword(s):  
River Valley ◽  
Broomcorn Millet ◽  
Huai River

scholarly journals Allelic Variation of Soybean Maturity Genes E1–E4 in the Huang-Huai-Hai River Valley and the Northwest China

Agriculture ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 478
Author(s):  
Xinyue Zhang ◽  
Tingting Wu ◽  
Huiwen Wen ◽  
Wenwen Song ◽  
Cailong Xu ◽  
...  
Keyword(s):  
Allelic Variation ◽  
Northwest China ◽  
River Valley ◽  
Natural Environments ◽  
Early Maturity ◽  
The World ◽  
Important Trait ◽  
Functional Alleles ◽  
Northwest Region ◽  
Yield Formation

Soybean is planted in a wide span of the world, and flowering and maturity time is an important trait determining soybean yield formation and adaptation. Maturity loci E1, E2, E3 and E4 were frequently reported as the most influential genetic loci for soybean flowering and maturity. To understand the allelic variation and assess the phenological traits of cultivars with different E allelic combinations in natural environments, 251 cultivars of maturity group (MG) I - V were field tested in 42 locations across four sub-regions in the Huang-Huai-Hai and Northwest region of China and genotyped with KASP markers for E1–E4 loci. The results indicated that mutant alleles were only found in the E1 and E2 locus, all of the cultivars carried functional alleles in the E3 and E4 loci in this area, with the frequency of mutant allele to be higher in early maturity groups (MGs) than late MGs. Among nine E allelic combinations in this area, one photoperiodic insensitive mutation in E2 loci (E1/e2-ns/E3-Ha/E4 and E1/e2-ns/E3-Mi/E4) made up the largest proportion (25.10 and 18.33%), while two photoperiodic insensitive mutations in both E1 and E2 loci (e1-as/e2-ns/E3-Ha/E4) (1.20%) occupied the lowest proportion in this panel. The major combinations of E locus for MGI, MGII and MG III in this area were E1/E2-dl/E3-Mi/E4, E1/e2-ns/E3-Mi/E4 and E1/e2-ns/E3-Ha/E4, respectively. Cultivars carrying e1-as/e2-ns/E3-Ha/E4 genotype flowered earliest (34 days) on average, 7.6 days earlier than the latest-flowering E haplotype (E1/e2-ns/E3-Ha/E4). This study provided an opportunity to detect the E allelic combinations in the Huang-Huai-Hai River Valley and the Northwest China, which would facilitate the improvement of soybean adaptation in the future.

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