HPLC-TOF/MS in identification of chemical constituents of Fuzhengpingxiao Capsule

2013 ◽  
Vol 32 (7) ◽  
pp. 770-779
Author(s):  
Liang ZHAO ◽  
Wen-jun TIAN ◽  
Lei LU ◽  
Hai ZHANG ◽  
Xin-xia WANG ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Tof Ms

Qualitative characterization of chemical constituents in Diwu-Yanggan-Fang by UPLC-Q-TOF/MS

2014 ◽  
Vol 6 (6) ◽  
pp. 725
Author(s):  
Chao Zhou ◽  
Hong-mei Jia ◽  
Hong-wu Zhang ◽  
Zhong-mei Zou
Keyword(s):  
Chemical Constituents ◽  
Tof Ms

scholarly journals Isolation and Identification of the Anti-Oxidant Constituents from Loropetalum chinense (R. Brown) Oliv. Based on UHPLC–Q-TOF-MS/MS

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1720 ◽  
Author(s):  
Haifang Chen ◽  
Mulan Li ◽  
Chen Zhang ◽  
Wendi Du ◽  
Haihua Shao ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Gallic Acid ◽  
Protocatechuic Acid ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Control Group ◽  
Ethyl Gallate ◽  
Isolation And Identification ◽  
Caffeoylquinic Acid ◽  
Tof Ms

The aim of this study was to identify the chemical constituents of Loropetalum chinense (R. Brown) Oliv. (LCO) and determine which of these had antioxidant effects. The chemical composition of a 70% ethanol extract of LCO was analyzed systematically using UHPLC–Q-TOF-MS/MS. The chemical components of the 70% ethanol extract of LCO were then separated and purified using macroporous resin and chromatographic techniques. Antioxidant activity was evaluated using a DPPH assay. In total, 100 compounds were identified tentatively, including 42 gallic acid tannins, 49 flavones, and 9 phenolic compounds. Of these, 7 gallium gallate, 4 flavonoid and 8 quinic acid compounds were separated and purified from the 70% ethanol extract of LCO. The compounds identified for the first time in LCO and in the genus Loropetalum were 3,4,5-trimethoxyphenyl-(6′-O-galloyl)-O-β-d-glucopyranoside, protocatechuic acid, ethyl gallate, 5-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 3,5-O-diocaffeoylquinic acid, 4,5-O-diocaffeoylquinic acid and 3,4-O-diocaffeoylquinic acid. The 50% inhibitory concentration (IC50) values of compounds 1,2,3,4,6-penta-O-galloyl-β-d-glucose, gallic acid, protocatechuic acid, and ethyl gallate were 1.88, 1.05, 1.18, and 1.05 μg/mL, respectively. Compared with the control group (VC) (2.08 μg/mL), these compounds exhibited stronger anti-oxidation activity. This study offered considerable insight into the chemical composition of LCO, with preliminary identification of the antioxidant ingredients.

scholarly journals Transformation Mechanisms of Chemical Ingredients in Steaming Process of Gastrodia elata Blume

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3159 ◽  
Author(s):  
Yun Li ◽  
Xiao-Qian Liu ◽  
Shan-Shan Liu ◽  
Da-hui Liu ◽  
Xiao Wang ◽  
...  
Keyword(s):  
Enzymatic Degradation ◽  
Chemical Constituents ◽  
Relative Content ◽  
Ester Bond ◽  
Gastrodia Elata ◽  
Before And After ◽  
Transformation Mechanisms ◽  
First Time ◽  
Hydroxybenzyl Alcohol ◽  
Tof Ms

To explore the transformation mechanisms of free gastrodin and combined gastrodin before and after steaming of Gastrodia elata (G. elata), a fresh G. elata sample was processed by the traditional steaming method prescribed by Chinese Pharmacopoeia (2015 version), and HPLC-ESI-TOF/MS method was used to identify the chemical composition in steamed and fresh G. elata. Finally, 25 components were identified in G. elata based on the characteristic fragments of the compounds and the changes of the 25 components of fresh and steamed G. elata were compared by the relative content. Hydrolysis experiments and enzymatic hydrolysis experiments of 10 monomer compounds simulating the G. elata steaming process were carried out for the first time. As a result, hydrolysis experiments proved that free gastrodin or p-hydroxybenzyl alcohol could be obtained by breaking ester bond or ether bond during the steaming process of G. elata. Enzymatic experiments showed that steaming played an important role in the protection of gastrodin, confirming the hypothesis that steaming can promote the conversion of chemical constituents of G. elata—inhibiting enzymatic degradation. This experiment clarified the scientific mechanism of the traditional steaming method of G. elata and provided reference for how to apply G. elata decoction to some extent.

Identification of chemical constituents in SiWu decoction by UHPLC-DAD-TOF/MS

2014 ◽  
Vol 26 (3) ◽  
pp. 517-537 ◽  
Author(s):  
M. Zhu ◽  
J. A. Duan ◽  
Y. P. Tang ◽  
J. M. Guo ◽  
E. X. Shang ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Tof Ms

UPLC-Q-TOF-MS/MS fingerprinting for rapid identification of the chemical constituents of Ermiao Wan

2015 ◽  
Vol 7 (3) ◽  
pp. 846-862 ◽  
Author(s):  
Guangli Yan ◽  
Di Zou ◽  
Aihua Zhang ◽  
Yunlong Tan ◽  
Hui Sun ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Rapid Identification ◽  
Determination Method ◽  
Tof Ms

In this work, a novel and efficient determination method for the rapid identification of multi-class chemical constituents of Ermiao Wan has been developed.

Analysis and identification of chemical constituents of fenugreek by UPLC-IT-MS n and UPLC-Q-TOF-MS

2017 ◽  
Vol 33 (5) ◽  
pp. 721-730 ◽  
Author(s):  
Jiaqi Wang ◽  
Wenyue Jiang ◽  
Zhongying Liu ◽  
Junhua Wang ◽  
Tianyue Fu ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Tof Ms

scholarly journals Rapid Screening and Quantitative Determination of Active Components in Qing-Hua-Yu-Re-Formula Using UHPLC-Q-TOF/MS and HPLC-UV

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Xin Shao ◽  
Jie Zhao ◽  
Xu Wang ◽  
Yi Tao
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Chemical Constituents ◽  
Rapid Screening ◽  
Active Components ◽  
Limit Of Quantification ◽  
Unknown Compound ◽  
Salvianolic Acid ◽  
Tof Ms

Qing-Hua-Yu-Re-Formula (QHYRF), a new herbal preparation, has been extensively used for treating diabetic cardiomyopathy. However, the chemical constituents of QHYRF remain uninvestigated. In the present study, rapid ultrahigh-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) was used to qualitatively analyze the components of QHYRF. Qualitative detection was performed on a Kromasil C18 column through the gradient elution mode, using acetonitrile-water containing 0.1% formic acid. Twenty-seven compounds were identified or tentatively characterized, including 12 phenolic acids, nine monoterpene glycosides, two flavonoids, three iridoids, and one unknown compound. Among these, six compounds were confirmed by comparing with standards. A high-performance liquid chromatography (HPLC) method was developed to simultaneously determine the following six active components in QHYRF: danshensu, paeoniflorin, acteoside, lithospermic acid, salvianolic acid B, and salvianolic acid C. These HPLC chromatograms were monitored at 254, 280, and 320 nm. The method was well validated with respect to specificity, linearity, limit of detection, limit of quantification, precision, stability, and recovery. The HPLC-UV method was successfully applied to 10 batches of QHYRF.

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