scholarly journals Wound Dressings Alter the Colony-Forming Efficiency of Keratinocytes in Cultured Sheet Grafts

2001 ◽  
Vol 10 (8) ◽  
pp. 749-754 ◽  
Author(s):  
Lea Ann DeGraffenried ◽  
R. Rivkah Isseroff
Keyword(s):  
Wound Dressings ◽  
Colony Forming Efficiency ◽  
Forming Efficiency

scholarly journals Influence of Glucose Concentration on Colony-Forming Efficiency and Biological Performance of Primary Human Tissue–Derived Progenitor Cells

Cartilage ◽  
2020 ◽  
pp. 194760352090660 ◽  
Author(s):  
Venkata P. Mantripragada ◽  
Ryan Kaplevatsky ◽  
Wes A. Bova ◽  
Cynthia Boehm ◽  
Nancy A. Obuchowski ◽  
...  
Keyword(s):  
Glucose Concentration ◽  
Pairwise Comparison ◽  
Culture Methods ◽  
Glucose Levels ◽  
Colony Forming Efficiency ◽  
Forming Efficiency ◽  
The Media ◽  
Outerbridge Grade ◽  
Biological Performance

Objective Glucose concentrations used in current cell culture methods are a significant departure from physiological glucose levels. The study focuses on comparing the effects of glucose concentrations on primary human progenitors (connective tissue progenitors [CTPs]) used for cartilage repair. Design Cartilage- (Outerbridge grade 1, 2, 3; superficial and deep zone cartilage), infrapatellar fatpad-, synovium-, and periosteum-derived cells were obtained from 63 patients undergoing total knee arthroplasty and cultured simultaneously in fresh chondrogenic media containing 25 mM glucose (HGL) or 5 mM glucose (NGL) for pairwise comparison. Automated ASTM-based quantitative image analysis was used to determine colony-forming efficiency (CFE), effective proliferation rates (EPR), and sulfated-proteoglycan (GAG-ECM) staining of the CTPs across tissue sources. Results HGL resulted in increased cell cultures with CFE = 0 compared with NGL in all tissue sources ( P = 0.049). The CFE in NGL was higher than HGL for superficial cartilage ( P < 0.001), and contrary for synovium-derived CTPs ( P = 0.046) when CFE > 0. EPR of the CTPs did not differ between the media in the 6-day assay time period ( P = 0.082). The GAG-ECM area of the CTPs and their progeny was increased in presence of HGL ( P = 0.027). Conclusion Glucose concentration is critical to progenitor’s physiology and should be taken into account in the setting of protocols for clinical or in vitro cell expansion strategies.

scholarly journals The Role of Nerve Growth Factor in Maintaining Proliferative Capacity, Colony-Forming Efficiency, and the Limbal Stem Cell Phenotype

Stem Cells ◽  
2018 ◽  
Vol 37 (1) ◽  
pp. 139-149 ◽  
Author(s):  
Sai Kolli ◽  
Sanja Bojic ◽  
Ali E. Ghareeb ◽  
Marzena Kurzawa-Akanbi ◽  
Francisco C. Figueiredo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cell Phenotype ◽  
Proliferative Capacity ◽  
Colony Forming Efficiency ◽  
Forming Efficiency ◽  
Limbal Stem Cell ◽  
Stem Cell Phenotype

scholarly journals Ageing and colony-forming efficiency of human hair follicle keratinocytes

2013 ◽  
Vol 22 (9) ◽  
pp. 604-606 ◽  
Author(s):  
Jennifer Lecardonnel ◽  
Nathalie Deshayes ◽  
Gaïanne Genty ◽  
Nathalie Parent ◽  
Bruno A. Bernard ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Human Hair ◽  
Human Hair Follicle ◽  
Colony Forming Efficiency ◽  
Forming Efficiency

In Vitro Proliferation of Various Human Dental Stem Cells

2007 ◽  
Vol 342-343 ◽  
pp. 165-168
Author(s):  
Sun Young Kook ◽  
You Young Jo ◽  
Hee Jung Lee ◽  
Byoung Moo Seo ◽  
Pill Hoon Choung
Keyword(s):  
Stem Cells ◽  
Stem Cell Marker ◽  
Human Stem Cells ◽  
Dental Stem Cells ◽  
In Vitro Proliferation ◽  
Human Teeth ◽  
Maxillary Bone ◽  
Colony Forming Efficiency ◽  
Forming Efficiency

To ascertain the properties of human dental stem cells, various postnatal human stem cells were isolated from extracted human teeth and jawbones. Isolated dental stem cells were plated until losing their ‘stemness’ and were evaluated for their proliferation rate, colony forming efficiency, and expression of a specific stem cell marker. These dental stem cells have the potential to proliferate for more than 10 passages, except for the maxillary bone marrow stem cells (MXBMSCs). In particular, stem cells obtained from the periapical follicle (PAFSCs) were definitely superior to the other dental stem cells in proliferation, colony forming efficiency and expression of specific stem cells marker.

Identification and characterization of SP cells in human lung adenocarcinoma SPC-A1 cells

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e22230-e22230
Author(s):  
Y. Zhu ◽  
L. Chen
Keyword(s):  
Lung Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Lung Adenocarcinoma ◽  
Human Lung ◽  
Serum Free ◽  
Human Lung Adenocarcinoma ◽  
Colony Forming Efficiency ◽  
Forming Efficiency ◽  
Human Lung Adenocarcinoma Cell

e22230 Background: There has been an increasing interest in recent years in the role stem cells play in health and disease. With an extensive understanding of their biology, a major role for stem cells in the malignant process has been proposed and the existence of cancer stem cells(CSCs) has been confirmed in hematopoietic malignancies, brain cancer, and solid organ malignancies including breast, prostate, colon, and pancreatic cancer. Lung cancer is the leading cause of cancer mortality in most large cities of China. It is possible that lung cancer contains cancer stem cells responsible for its malignancy. The aim of this study is to identify, characterize and enrich the CSC population that drives and maintains lung adenocarcinoma growth and metastasis. Methods: Side population (SP) cell analysis and sorting were applied to established human lung adenocarcinoma cell line and an attempt to further enrich them by preliminary serum-free culture before fluorescence activated cell sorting(FACS) was done. Stem cell properties of SP cells were evaluated by their proliferative index, colony-forming efficiency, tumorigenic potential, bi-differentiation capacity and the expression of common stem cell surface markers. Results: Lung cancer cells could grow in a serum-free Medium (SFM) as non-adherent spheres similar to neurospheres or mammospheres. The proportion of SP cells in cell spheres was significantly higher than that in cells grown as monolayers. SP cells had a greater proliferative index, a higher colony-forming efficiency and a greater ability to form tumor in vivo. SP cells were both CCA positive and SP-C positive while non-SP cells were only SP-C positive. Flow cytometric analysis of cell phenotyping showed that SP cells expressed CD133 and CD44, the common cell surface markers of cancer stem cells, while non-SP cells only expressed CD44. Conclusions: SP cells existed in human lung adenocarcinoma cell lines and they could be further enriched by preliminary serum-free culture before FACS sorting. SP cells possessed the properties of cancer stem cells. No significant financial relationships to disclose.

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