Synergistic Methodology Based on Ion Exchange and Biodegradation Mechanisms Applied for Metal Complex Dye Removal from Waste Waters

2018 ◽  
Vol 69 (1) ◽  
pp. 38-44
Author(s):  
Nicoleta Mirela Marin ◽  
Olga Tiron ◽  
Luoana Florentina Pascu ◽  
Mihaela Costache ◽  
Mihai Nita Lazar ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Synergistic Effects ◽  
Freundlich Isotherm ◽  
White Rot ◽  
White Rot Fungus ◽  
Basic Anion Exchange Resin ◽  
Km Value ◽  
Points Of View ◽  
Basic Anion

This study investigates the synergistic effects of ion exchange and biodegradation methods to remove the Acid Blue 193 also called Gryfalan Navy Blue RL (GNB) dye from wastewater. Ion exchange studies were performed using a strongly basic anion exchange resin Amberlite IRA 400. The equilibrium was characterized by a kinetic and thermodynamic points of view, establishing that the sorption of the GNB dye was subject to the Freundlich isotherm model with R2 = 0.8710. Experimental results showed that the activated resin can removed up to 93.4% when the concentration of dye solution is 5.62�10-2 mM. The biodegradation of the GNB was induced by laccase, an enzyme isolated from white-rot fungus. It was also analyzed the role of pH and dye concentration on GNB biodegradation, so 5�10-2 mM dye had a maximum discoloration efficiency of 82.9% at pH of 4. The laccase showed a very fast and robust activity reaching in a few minutes a Km value of 2.2�10-1mM. In addition, increasing the GNB concentration up to 8�10-1 mM did not triggered a substrat inhibition effect on the laccase activity. Overall, in this study we proposed a mixt physicochemical and biological approach to enhance the GNB removal and biodegradability from the wastewaters and subsequently the environment.

Automated Carbohydrate Analysis of Physiologic Fluids

1968 ◽  
Vol 14 (6) ◽  
pp. 538-547 ◽  
Author(s):  
R L Jolley ◽  
M L Freeman
Keyword(s):  
Blood Serum ◽  
Research Laboratory ◽  
Exchange Resin ◽  
Clinical Laboratory ◽  
Anion Exchange Resin ◽  
Internal Standard ◽  
Carbohydrate Analysis ◽  
Basic Anion Exchange Resin ◽  
Basic Anion

Abstract Automated carbohydrate analysis can be useful clinically in the research laboratory as an aid in understanding the fundamental role of carbohydrates in metabolism, including their pathologic significance. An analytic system being developed at our laboratory utilizes an automated carbohydrate analyzer to chromatograph borated physiologic fluids while using strongly basic anion-exchange resin. The eluted carbohydrates are detected by sulfuric acid-phenol colorimetry. The carbohydrate analyzer used in this experimental work should become a useful tool in the clinical laboratory. Normal and diabetic human urine and blood serum have been chromatographed and significant differences established. As many as 38 peaks have been observed in the complex urine chromatograms. Using cochromatographic technics, 14 peaks have been tentatively identified as sucrose, raffinose, N-acetylglucosamine, maltose, lactose, ribose, fructose, arabinose, fucose, galactose, xylose, mannoheptulose, glucose, and glucose-1-phosphate. All except fucose have been quantified. Blood serum chromatograms consist of a major glucose peak and several smaller peaks indicating traces of other sugars. Melibiose has been used as an internal standard in chromatograms to determine recovery, resolution, and reproducibility. With the present technic, the lower limit of detectable sugar in physiologic fluids is in the 1-µg. range.

Investigation of the role of 3-hydroxyanthranilic acid in the degradation of lignin by white-rot fungus Pycnoporus cinnabarinus

2001 ◽  
Vol 28 (4-5) ◽  
pp. 301-307 ◽  
Author(s):  
Kaichang Li ◽  
Peter S Horanyi ◽  
Robert Collins ◽  
Robert S Phillips ◽  
Karl-Erik L Eriksson
Keyword(s):  
White Rot ◽  
White Rot Fungus ◽  
Pycnoporus Cinnabarinus ◽  
Hydroxyanthranilic Acid

Bifunctional Ion Exchange Resin with Thiol and Quaternary Ammonium Groups for the Sorption of Arsenate

2003 ◽  
Vol 68 (11) ◽  
pp. 2159-2170 ◽  
Author(s):  
Martin Hrubý ◽  
Viktorya Korostyatynets ◽  
Milan J. Beneš ◽  
Zdeněk Matějka
Keyword(s):  
Ion Exchange ◽  
Quaternary Ammonium ◽  
Exchange Resin ◽  
Direct Conversion ◽  
Ion Exchange Resin ◽  
Basic Anion Exchange Resin ◽  
Styrene Divinylbenzene ◽  
Basic Anion ◽  
Isothiuronium Salt ◽  
Sulfur Containing

A bifunctional resin with thiol and quaternary ammonium groups was prepared from a macroporous strongly basic anion exchange resin of the styrene-divinylbenzene type. The key step of the synthesis is the reaction of the quaternary benzyltrimethylammonium group of the ion exchange resin with suitable sulfur-containing nucleophiles. Three synthetic routes are described: direct conversion to thiol with sodium sulfide, reaction with potassium O-ethyl dithiocarbonate followed by decomposition of the dithiocarbonate with ammonia or acid and conversion to polymeric isothiuronium salt by the reaction with thiourea and subsequent alkaline hydrolysis to thiol. Sorption of arsenate on thiol resin and its Fe(III) complex was studied.

scholarly journals Succession and potential role of bacterial communities during Pleurotus ostreatus production

2021 ◽  
Author(s):  
Renáta Bánfi ◽  
Zsuzsanna Pohner ◽  
Attila Szabó ◽  
Gábor Herczeg ◽  
Gábor M Kovács ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Pleurotus Ostreatus ◽  
Sharp Decrease ◽  
Amplicon Sequencing ◽  
White Rot ◽  
White Rot Fungus ◽  
Rrna Gene ◽  
Fungal Interactions ◽  
Body Production

ABSTRACT There is an increasing interest in studying bacterial-fungal interactions (BFIs), also the interactions of Pleurotus ostreatus, a model white-rot fungus and important cultivated mushroom. In Europe, P. ostreatus is produced on a wheat straw-based substrate with a characteristic bacterial community, where P. ostreatus is exposed to the microbiome during substrate colonisation. This study investigated how the bacterial community structure was affected by the introduction of P. ostreatus into the mature substrate. Based on the results obtained, the effect of the presence and absence of this microbiome on P. ostreatus production in an experimental cultivation setup was determined. 16S rRNA gene-based terminal restriction fragment length polymorphism (T-RFLP) and amplicon sequencing revealed a definite succession of the microbiome during substrate colonisation and fruiting body production: a sharp decrease in relative abundance of Thermus spp. and Actinobacteria, and the increasing dominance of Bacillales and Halomonas spp. The introduced experimental cultivation setup proved the protective role of the microbial community against competing fungi without affecting P. ostreatus growth. We could also demonstrate that this effect could be attributed to both living microbes and their secreted metabolites. These findings highlight the importance of bacterial-fungal interactions during mushroom production.

scholarly journals Role of P450 Monooxygenases in the Degradation of the Endocrine-Disrupting Chemical Nonylphenol by the White Rot Fungus Phanerochaete chrysosporium

2009 ◽  
Vol 75 (17) ◽  
pp. 5570-5580 ◽  
Author(s):  
Venkataramanan Subramanian ◽  
Jagjit S. Yadav
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Protein Identification ◽  
Enzyme Inhibitor ◽  
White Rot ◽  
White Rot Fungus ◽  
Malt Extract ◽  
P450 Monooxygenase ◽  
Genome Wide ◽  
A Genome

ABSTRACT The white rot fungus Phanerochaete chrysosporium extensively degraded the endocrine disruptor chemical nonylphenol (NP; 100% of 100 ppm) in both nutrient-limited cultures and nutrient-sufficient cultures. The P450 enzyme inhibitor piperonyl butoxide caused significant inhibition (∼75%) of the degradation activity in nutrient-rich malt extract (ME) cultures but no inhibition in defined low-nitrogen (LN) cultures, indicating an essential role of P450 monooxygenase(s) in NP degradation under nutrient-rich conditions. A genome-wide analysis using our custom-designed P450 microarray revealed significant induction of multiple P450 monooxygenase genes by NP: 18 genes were induced (2- to 195-fold) under nutrient-rich conditions, 17 genes were induced (2- to 6-fold) in LN cultures, and 3 were induced under both nutrient-rich and LN conditions. The P450 genes Pff 311b (corresponding to protein identification number [ID] 5852) and Pff 4a (protein ID 5001) showed extraordinarily high levels of induction (195- and 167-fold, respectively) in ME cultures. The P450 oxidoreductase (POR), glutathione S-transferase (gst), and cellulose metabolism genes were also induced in ME cultures. In contrast, certain metabolic genes, such as five of the peroxidase genes, showed partial downregulation by NP. This study provides the first evidence for the involvement of P450 enzymes in NP degradation by a white rot fungus and the first genome-wide identification of specific P450 genes responsive to an environmentally significant toxicant.

Sign in / Sign up

Export Citation Format

Share Document