scholarly journals Validation of a Quantitative Analysis Method for Collagen Extracted from Grey Mullet Marine Fish

2019 ◽  
Vol 70 (3) ◽  
pp. 835-842
Author(s):  
Rodica Sirbu ◽  
Gabriela Stanciu ◽  
Emin Cadar ◽  
Aneta Tomescu ◽  
Melat Cherim

The Black Sea offers numerous harnessing possibilities for the medical and pharmaceutical, agricultural, food industry and cosmetic fields. Collagen extraction from the Black Sea fish is a research area of great interest. The purpose of this paper is to optimize the collagen quantitative analysis method based on hydroxyproline reagent through visible molecular absorption spectrometry. The adapted method was validated, achieving the following performance criteria: linearity, detection and quantification limits, accuracy/fidelity, stability/sturdiness, repeatability, and measurement uncertainty. The validated method was applied for the quantitative determination of collagen content in Grey Mullet fish and for the evaluation of collagen extraction output.

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Fengdan Jin

The glucan-Fe3O4magnetic nanoparticles were prepared by hydrothermal method. The mixture of FeCl2and glucan was stirred vigorously for half an hour under low temperature (15°C). KOH of 1 mol/L was dropwise added, slowly, into the solution until the pH to 12. Immediately, KNO3was added and the temperature was raised to 75°C for an hour. All the processes of Fe3O4crystal particles generation were under nitrogen. An atomic absorption spectrometry quantitative analysis method was built to determine the in vivo distribution of the glucan-Fe3O4magnetic nanoparticles in mice. The diameter of glucan-Fe3O4magnetic nanoparticles was about 25 nm and they were up taken by the liver primarily after intravenous administration via the tail.


2018 ◽  
Vol 33 (7) ◽  
pp. 1184-1195 ◽  
Author(s):  
Jianhong Yang ◽  
Xiaomeng Li ◽  
Huili Lu ◽  
Jinwu Xu ◽  
Haixia Li

Information learnt from spectra at room temperature is transferred to assist in building a better regression model at high temperature.


2014 ◽  
Vol 6 (11) ◽  
pp. 3763-3771 ◽  
Author(s):  
Jung-Hoon Kim ◽  
Hyeun-Kyoo Shin ◽  
Chang-Seob Seo

The quantification of 12 marker compounds in palmijihwang-hwan using a HPLC-PDA with a reversed-phase C18column and an amino column.


2003 ◽  
Vol 50 (4) ◽  
pp. 188-192 ◽  
Author(s):  
Hiroshi Yada ◽  
Nobuki Gato ◽  
Hidenori Nagatomo ◽  
Yoshihiro Chuda ◽  
Hiroshi Ono ◽  
...  

2017 ◽  
Vol 21 (3) ◽  
pp. 317
Author(s):  
Agussalim M ◽  
Hapzi Ali

This study aims to analyze the effect of product quality and service quality on brand image and customer satisfaction either partially or simultaneously on Giant Citra Raya Tangerang. The population of this study is Giant Citra Raya customers which averages 2,070,823 / 12/30 = 5752 per day. The sample in this research using Slovin formula is 152 customers. Quantitative analysis method using path analysis, followed by determination analysis (R Square), partial hypothesis testing (t test) and simultaneous (F test) with alpha 5 percent. Before further analyzed first tested the instrument and test the classical assumption. Analytical tool using SPSS version 21.0 for windows. The result of the research shows that product quality and service quality are partially and simultaneously have positive and significant effect on brand image and customer satisfaction, and brand image has influence to customer satisfaction at Giant Citra Raya Tangerang.


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