In-vitro Investigation of the Effect of Bee Venom on Schistosoma mansoni Eggs

2020 ◽  
Keyword(s):  
Schistosoma Mansoni ◽  
Critical Concentration ◽  
Lethal Effect ◽  
Bee Venom ◽  
Morphological Alterations ◽  
In Vitro Investigation ◽  
Percent Mortality ◽  
Parasitic Worms ◽  
Vitro Effect

Schistosomiasis (Bilharziasis) is a fatal parasitic disease caused by parasitic worms with the genus Schistosoma. The release of Schistosoma eggs in running fresh water contributes to completing its life cycle. Therefore, finding a suitable drug having ovicidal activity towards eggs is crucial. Here, we investigate the in-vitro effect of bee venom (the venom of Apis millifera) on the eggs of Schistosoma mansoni (S. mansoni). The eggs were incubated with different concentrations of bee venom and then the percent mortality, hatchability and morphology of the eggs were observed. It was found that bee venom causes morphological alterations for S. mansoni eggs. In addition, there is a critical concentration (100 µg/mL) at which bee venom leads to the lowest mortality and the highest hatchability percent. Below or above this threshold, the mortality increases and the hatchability decreases. Moreover, bee venom was proven to have a lethal effect on S. mansoni miracidia.

scholarly journals Activity of Combinations of Antioxidants and Anthelmintic Drugs against the Adult Stage of Schistosoma mansoni

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Maria João Gouveia ◽  
Paul J. Brindley ◽  
Fátima Gärtner ◽  
Nuno Vale
Keyword(s):  
Schistosoma Mansoni ◽  
Therapeutic Approach ◽  
Adult Stage ◽  
Morphological Alterations ◽  
Single Drug ◽  
Antischistosomal Activity ◽  
Per Se ◽  
And Control ◽  
Novel Therapeutic

Schistosomiasis remains a major neglected tropical disease. The treatment and control of schistosomiasis rely on a single drug, praziquantel (PZQ). Despite its efficacy, treatment with PZQ presents some major drawbacks including an inability of the chemotherapy to reverse disease-induced fibrosis and the prospect of the emergence of drug resistance. Here, we investigated a novel therapeutic approach with antioxidant biomolecules in combination with PZQ against the adult developmental stage of Schistosoma mansoni and oviposition in vitro, given that this therapeutic approach achieved synergistic/additive activity against larval schistosomes. The antioxidants curcumin and oxadiazole per se exhibited antischistosomal activity against adult worms leading to severe morphological alterations and death. Additionally, the antioxidant flavone combined with vandetanib or imatinib improved antischistosomal activity against adult forms. By contrast, however, these antioxidant-anthelmintic combinations were not as effective against adults in comparison to larval schistosomes. Nevertheless, the antioxidants alone or combined with drugs inhibited oviposition.

In vitro effect of Myrrh extracts on the viability of Schistosoma mansoni larvae

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
SD Karamustafa ◽  
N Mansour ◽  
B Demirci ◽  
A Ankli ◽  
KHC Başer ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Vitro Effect

scholarly journals Potentiation of Bleomycin Lethality in HeLa and V79 Cells by Bee Venom

2009 ◽  
Vol 60 (3) ◽  
pp. 317-326 ◽  
Author(s):  
Nada Oršolić
Keyword(s):  
Hela Cells ◽  
Antitumor Agents ◽  
Potent Inhibitor ◽  
Lethal Dose ◽  
Lethal Effect ◽  
Bee Venom ◽  
Cell Counting ◽  
V79 Cells ◽  
Antitumour Agents

Potentiation of Bleomycin Lethality in HeLa and V79 Cells by Bee VenomThis study investigated possible growth-inhibiting effects of bee venom applied alone or in combination with a cytotoxic drug bleomycin on HeLa and V79 cells in vitro based on clone formation, cell counting, and apoptosis. Melittin, the key component of bee venom, is a potent inhibitor of calmodulin activity, and also a potent inhibitor cell growth and clonogenicity. Intracellular accumulation of melittin correlates with the cytotoxicity of antitumour agents. Previous studies indicated that some calcium antagonists and calmodulin inhibitors enhanced intracellular levels of antitumor agents by inhibiting their outward transport. In this study, treatment of exponentially growing HeLa and V79 cells with bleomycin caused a dose-dependent decrease in cell survival due to DNA damage. This lethal effect was potentiated by adding a non-lethal dose of the bee venom. By preventing repair of damaged DNA, bee venom inhibited recovery from potentially lethal damage induced by bleomycin in V79 and HeLa cells. Apoptosis, necrosis, and lysis were presumed as possible mechanisms by which bee venom inhibited growth and clonogenicity of V79 cells. HeLa cells, on the other hand, showed greater resistance to bee venom. Our findings suggest that bee venom might find a therapeutic use in enhancing cytotoxicity of antitumour agent bleomycin.

scholarly journals In vitro Effect of Mefloquine on Adult Schistosoma mansoni

2015 ◽  
Vol 10 (3) ◽  
pp. 111-119 ◽  
Author(s):  
Samar Sayed Attia ◽  
Omaima Mohammed Abou-Shady ◽  
Soheir Sayed Mohammed ◽  
Hebat-Allah Salah Yusuf ◽  
Dina Omar Helmy
Keyword(s):  
Schistosoma Mansoni ◽  
Vitro Effect

scholarly journals Combination Anthelmintic/Antioxidant Activity Against Schistosoma Mansoni

Biomolecules ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 54 ◽  
Author(s):  
Maria João Gouveia ◽  
Paul J. Brindley ◽  
Gabriel Rinaldi ◽  
Fátima Gärtner ◽  
José Manuel Correia da Costa ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Daily Basis ◽  
Biological Properties ◽  
Novel Therapy ◽  
Morphological Alterations ◽  
Valuable Adjunct ◽  
In Vitro Drug Screening ◽  
Antischistosomal Activity ◽  
Anthelmintic Drugs

Schistosomiasis is a major neglected tropical disease. Treatment for schistosomiasis with praziquantel (PZQ), which is effective against the parasite, by itself is not capable to counteract infection-associated disease lesions including hepatic fibrosis. There is a pressing need for novel therapies. Due to their biological properties, antioxidant biomolecules might be useful in treating and reverting associated pathological sequelae. Here, we investigated a novel therapy approach based on a combination of anthelmintic drugs with antioxidant biomolecules. We used a host-parasite model involving Bioamphalaria glabrata and newly transformed schistosomula (NTS) of Schistosoma mansoni. For in vitro drug screening assays, was selected several antioxidants and evaluated not only antischistosomal activity but also ability to enhance activity of the anthelmintic drugs praziquantel (PZQ) and artesunate (AS). The morphological alterations induced by compounds alone/combined were assessed on daily basis using an inverted and automated microscope to quantify NTS viability by a fluorometric-based method. The findings indicated that not only do some antioxidants improve antischistosomal activity of the two anthelmintics, but they exhibit activity per se, leading to high mortality of NTS post-exposure. The combination index (CI) of PZQ + Mel (CI = 0.80), PZQ + Resv (CI = 0.74), AS + Resv (CI = 0.34), AS + NAC (CI = 0.89), VDT + Flav (CI = 1.03) and VDT + Resv (CI = 1.06) reveal that they display moderate to strong synergism. The combination of compounds with discrete mechanisms of action might provide a valuable adjunct to contribution for treatment of schistosomiasis-associated disease.

Spotlight on the in vitro effect of artemisinin–naphthoquine phosphate on Schistosoma mansoni and its snail host Biomphalaria alexandrina

Acta Tropica ◽  
2015 ◽  
Vol 141 ◽  
pp. 37-45 ◽  
Author(s):  
Samar N. El-Beshbishi ◽  
Samia El Bardicy ◽  
Menerva Tadros ◽  
Magda Ayoub ◽  
Amira Taman
Keyword(s):  
Schistosoma Mansoni ◽  
Snail Host ◽  
Biomphalaria Alexandrina ◽  
Vitro Effect

The in vitro Effect of Normal and Immune Serum upon the Cercariæ of Schistosoma mansoni

1952 ◽  
Vol 26 (1) ◽  
pp. 25-42 ◽  
Author(s):  
O. D. Standen
Keyword(s):  
Schistosoma Mansoni ◽  
Worm Burden ◽  
Immune Serum ◽  
Vitro Effect ◽  
Nematode Worm

The majority of normal sera proved in vitro to be cercaricidal to larvae of S. mansoni. Of 502 normal sera. 21 per cent, were noncercaricidal whereas the remainder exhibited varying degrees of cercaricidal potency.The cercaricidal factor was thermolabile and disappeared during storage. It bore several points of similarity to complement.The cercaricidal factor was present to a varying degree in sera from all species investigated. Apparently normal sera from all cattle and different proportions of other species, caused an enveloping precipitate to form around the cercariae. This bore no obvious relationship to the nematode worm burden or presence of F. hepatica.An enveloping precipitate formed around cercariae of S. mansoni when placed in vitro in immune sera and incubated at 87°C. This occurred in serum immune to S. haematobium as well as to S. mansoni. It is not known-whether age and weight of infection influence the rate and extent of envelope precipitation.

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