Method Validation for Determination of Lignan Content in Fermented Sesame by Bioconversion

Determination of serum or plasma progesterone (P4) concentrations is important to recognize pregnant and non-pregnant ewes, and also to predict the number of carried lambs. The 2 most common methodologies for the detection of plasma P4 are radioimmunoassay (RIA) and enzyme immunoassay (EIA). RIA is very expensive, and not all laboratories are equipped to perform this test; EIA is commercially available for human use, but only a few companies produce species-specific kits, which are expensive. We verified for ovine plasma a less expensive and easily available ELISA kit (DiaMetra) designed to quantify P4 in humans. Pools of ovine and human plasma were used to compare repeatability, accuracy, sensitivity, and stability of P4 measured by the DiaMetra kit. Repeatability data were within 15%, and accuracy values were ~90% for both plasma matrices. Stability data showed a loss of <20% for freeze–thaw and <30% for 30-d storage. All parameters were acceptable under international guidelines for method validation. The human ELISA kit was used successfully to quantify plasma P4 in 26 ewes during pregnancy until delivery. P4 concentrations were also correlated with the number of carried lambs.

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Analytical Method Validation and Determination of Iron and Phosphorus in Vegetable Oil by Inductively Coupled Plasma–Mass Spectrometry with Microwave Assisted Digestion

Analytical Letters ◽

10.1080/00032719.2017.1387554 ◽

2018 ◽

Vol 51 (11) ◽

pp. 1774-1788 ◽

Cited By ~ 2

Author(s):

Rizwan Shariff ◽

Ayyappan A. Aachary ◽

Lawrence H. Pacquette ◽

Ashutosh K. Mittal ◽

Rajesh Girdhar

Keyword(s):

Mass Spectrometry ◽

Method Validation ◽

Analytical Method ◽

Inductively Coupled Plasma ◽

Analytical Method Validation ◽

Inductively Coupled ◽

Microwave Assisted ◽

Plasma Mass Spectrometry ◽

Microwave Assisted Digestion

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Method Validation and Determination of Leachable Metals from Infusion and Transfusion Medical Devices

Brazilian Journal of Analytical Chemistry ◽

10.30744/brjac.2179-3425.ar-67-2021 ◽

2021 ◽

Author(s):

Alexandra Schuh ◽

Dirce Pozebon

Keyword(s):

Method Validation ◽

Medical Devices ◽

Inductively Coupled Plasma ◽

Optical Emission ◽

Emission Spectrometry ◽

Icp Oes ◽

Inductively Coupled ◽

Sample Extract ◽

Plasma Optical Emission Spectrometry

This work deals with method validation for regulated metals (Cd, Pb, Ba, Sn, Cr and Cu) determination in infusion and transfusion medical devices. The investigated metals were extracted with water at (37 ± 1) °C followed by their determination in the extract by using inductively coupled plasma optical emission spectrometry (ICP-OES). The validated method was applied in the analysis of infusion and transfusion devices commercialized in Brazil to verify compliance with current legislation, which establishes that the sum of Pb, Ba, Sn, Cr and Cu in the extract must not exceed 1 mg L-1 and that of Cd must not 0.1 mg L-1. Samples from five manufacturers of infusion and transfusion devices, produced in Brazil or imported, were analysed. The results of the analysis showed that all devices complied with the legislation, whereas the sum of Pb, Ba, Sn, Cr and Cu concentrations and that of Cd in the extract were lower than the maximum permissible; Cd was not detected in any sample extract and the sum of the other elements was < 0.14 mg L-1 in all extracts of the analysed samples.

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Method Validation for the Determination of Phthalates in Indoor Air by GC-MS with Solid-Phase Adsorption/Solvent Extraction using Octadecyl Silica Filter and Styrene–Divinylbenzene Copolymer Cartridge

BPB Reports ◽

10.1248/bpbreports.2.5_86 ◽

2019 ◽

Vol 2 (5) ◽

pp. 86-90

Author(s):

Toshiko Tanaka-Kagawa ◽

Ikue Saito ◽

Aya Onuki ◽

Maiko Tahara ◽

Tsuyoshi Kawakami ◽

...

Keyword(s):

Solvent Extraction ◽

Method Validation ◽

Indoor Air ◽

Solid Phase ◽

Octadecyl Silica ◽

Styrene Divinylbenzene ◽

Solid Phase Adsorption

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Method validation for determination of amino acids in feed by UPLC

Accreditation and Quality Assurance ◽

10.1007/s00769-017-1281-9 ◽

2017 ◽

Vol 22 (5) ◽

pp. 247-252 ◽

Cited By ~ 11

Author(s):

Katarzyna Szkudzińska ◽

Ilona Smutniak ◽

Jolanta Rubaj ◽

Waldemar Korol ◽

Grażyna Bielecka

Keyword(s):

Amino Acids ◽

Method Validation

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Simultaneous determination of duloxetine and 4-hydroxy duloxetine glucuronide in human plasma and back-conversion study

Bioanalysis ◽

10.4155/bio-2021-0185 ◽

2021 ◽

Author(s):

Gabriel Onn Kit Loh ◽

Emily Yii Ling Wong ◽

Yvonne Tze Fung Tan ◽

Yi Lin Lee ◽

Chun Keat Chew ◽

...

Keyword(s):

Simultaneous Determination ◽

Human Plasma ◽

Method Validation ◽

Bioequivalence Study ◽

Stability Study ◽

Bioanalytical Method Validation ◽

Study Materials ◽

Study Results ◽

Back Conversion

Aim: To develop an LC-MS/MS method for simultaneous determination of duloxetine and its metabolite, 4-hydroxy duloxetine glucuronide (4HDG) in human plasma and to investigate the potential back-conversion of 4HDG to duloxetine using stability study. Materials & methods: The LC-MS/MS method was validated according to the EMA and USFDA Bioanalytical Method Validation Guidelines and applied to pilot bioequivalence study. Results & conclusion: The method validation results were within the acceptance limits. The stability study and incurred sample reanalysis results ruled out the occurrence of back-conversion. The study highlighted the conduct of back-conversion test and the advantages of LC-MS/MS method in terms of sensitivity, specificity and low consumption of organic solvents.

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