Correction to “Effect of probiotic Lactobacillus plantarum Dad-13 powder consumption on the gut microbiota and intestinal health of overweight adults”. World J Gastroenterol 2021; 27(1): 107-128 [PMID: 33505154 DOI: 10.3748/wjg.v27.i1.107]

Abstract Background: Coccidiosis is one of the major parasitic diseases in the commercial broiler industry. Probiotics can protect poultry against Eimeria infection. However, the mechanisms are not fully known. Therefore, Lactobacillus plantarum P8 (P8) was used to investigate its anti-coccidial property and mechanism.Methods: Five hundred broilers were allocated to five treatments: control diet (NC), control diet + Eimeria infection (IC), control diet containing 1 × 107 cfu/g P8 + Eimeria infection (P8L), control diet containing 1 × 108 cfu/g P8 + Eimeria infection (P8H), and control diet + Eimeria infection + Diclazuril (DIC). At day 14, all treatments except NC were inoculated with sporulated oocysts. Results: Eimeria infection increased the mortality and oocysts shedding, and declined the growth performance as well as the intestinal barrier in Eimeria-treated broilers. On the contrary, dietary supplementation of low level P8, high level P8 and DIC decreased the mortality and oocysts shedding, but improved the growth performance and intestinal barrier. The impaired intestinal morphology in the IC group was also improved by P8H and DIC treatments. Besides, the elevated oxidative stress and pro-inflammation in Eimeria-infected broilers were reduced by P8L, P8H and DIC treatments. Metagenomic analysis indicated P8 altered the structure of the gut microbiota, and the alteration was more obvious at day 21 than day 42. Notably, IC also increased the abundances of Eimeriidae, Eimeria and Eimeria tenella at day 21, while P8L and DIC decreased the abundances. Correlation analysis revealed that bacteria in Eimeria-treated broilers positively correlated with the intestinal permeability, oxidative stress and inflammation, while bacteria in broilers receiving P8L and DIC negatively correlated with the aforementioned pathological indices. Functional prediction demonstrated that the metagenomes of Eimeria-infected broilers were involved in several diseases. But the metagenomes of P8L-treated broilers were involved in energy metabolism and replication repair. Conclusions: Dietary P8 supplementation inhibited oocyst shedding and improved the growth performance as well as the intestinal health of broilers infected with Eimeria, which was closely related to the regulation of gut microbiota. Moreover, the effects of P8 may be more effective in the early infection of coccidia.

Download Full-text

Universal adaptive evolution strategy of probiotic Lactobacillus plantarum to gut selection pressure from humans, mice and zebrafish

10.21203/rs.3.rs-106737/v1 ◽

2021 ◽

Author(s):

Shi Huang ◽

Shuaiming Jiang ◽

Dongxue Huo ◽

Celeste Allaband ◽

Mehrbod Estaki ◽

...

Keyword(s):

Gut Microbiota ◽

Lactobacillus Plantarum ◽

Ex Vivo ◽

Acid Tolerance ◽

Adaptive Strategy ◽

Adaptation Strategy ◽

Carbohydrate Utilization ◽

Healthy Humans ◽

Probiotic Lactobacillus

Abstract Improving the probiotic engraftment in humans requires a thorough understanding of in vivo gut-adaptive strategy of probiotics in diverse contexts. Here, we exposed the probiotic Lactobacillus plantarum HNU082 (Lp082) to gut microbiota of healthy humans, mice, and zebrafish for four weeks. Independent of host choice, Lp082 established and adapted the gut by acquiring highly consistent single-nucleotide mutations, which modulated carbohydrate utilization, and acid tolerance, and significantly promoted its in vivo competitive fitness. In turn, resident gut microbial strains, especially competing strains with Lp082 (e.g. Bacteroides spp. and Bifidobacterium spp.), actively respond to Lp082 engraftment by accumulating 10-70 folds more evolutionary changes than usual. Human gut microbiota exhibited a higher ecological and genetic stability than that of mice. Collectively, the highly convergent adaptation strategy of Lp082 across host environments lay the foundation for leveraging the animal model for ex vivo engineering of probiotics for better engraftment outcomes in humans.

Download Full-text

Protective Effect of Lactobacillus plantarum P8 on Growth Performance, Intestinal Health, and Microbiota in Eimeria-Infected Broilers

Frontiers in Microbiology ◽

10.3389/fmicb.2021.705758 ◽

2021 ◽

Vol 12 ◽

Author(s):

Yang Wang ◽

Xiaoguo Lv ◽

Xuemin Li ◽

Jinshan Zhao ◽

Kai Zhang ◽

...

Keyword(s):

Oxidative Stress ◽

Gut Microbiota ◽

Growth Performance ◽

Lactobacillus Plantarum ◽

Control Diet ◽

Intestinal Barrier ◽

Intestinal Morphology ◽

Intestinal Health ◽

Commercial Broiler ◽

High Level

Coccidiosis is one of the major parasitic diseases in the commercial broiler industry. Probiotics can protect poultry against Eimeria infection. However, the mechanisms are not fully known. Therefore, Lactobacillus plantarum P8 (P8) was used to investigate its anti-coccidial property and mechanism. Five hundred broilers were allocated to five treatments: control diet (NC), control diet + Eimeria infection (IC), control diet containing 1 × 107 cfu/g P8 + Eimeria infection (P8L), control diet containing 1 × 108 cfu/g P8 + Eimeria infection (P8H), and control diet + Eimeria infection + Diclazuril (DIC). At day 14, all treatments except NC were inoculated with sporulated oocysts. Results indicated that Eimeria infection increased the mortality and oocysts shedding, and declined the growth performance as well as the intestinal barrier in Eimeria-treated broilers. On the contrary, dietary supplementation of low level P8, high level P8 and DIC decreased the mortality and oocysts shedding, but improved the growth performance and intestinal barrier. The impaired intestinal morphology in the IC group was also improved by P8H and DIC treatments. Besides, the elevated oxidative stress and pro-inflammation in Eimeria-infected broilers were reduced by P8L, P8H, and DIC treatments. Metagenomic analysis indicated P8 altered the structure of the gut microbiota, and the alteration was more obvious at day 21 than day 42. Notably, IC also increased the abundances of Eimeriidae, Eimeria and Eimeria tenella at day 21, while P8L and DIC decreased the abundances. Correlation analysis revealed that bacteria in Eimeria-treated broilers positively correlated with the intestinal permeability, oxidative stress and inflammation, while bacteria in broilers receiving P8L and DIC negatively correlated with the aforementioned pathological indices. Functional prediction demonstrated that the metagenomes of Eimeria-infected broilers were involved in several diseases. But the metagenomes of P8L-treated broilers were involved in energy metabolism and replication repair. In conclusion, dietary P8 supplementation inhibited oocyst shedding and improved the growth performance as well as the intestinal health of broilers infected with Eimeria, which was closely related to the regulation of gut microbiota. Moreover, the effects of P8 may be more effective in the early infection of coccidia.

Download Full-text

Effects of Consumption of Probiotic Powder Containing Lactobacillus Plantarum Dad-13 on Fecal Bacterial Population in School-Age Children in Indonesia

International Journal of Probiotics and Prebiotics ◽

10.37290/ijpp2641-7197.14:1-8 ◽

2019 ◽

Vol 14 (1) ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Banin Maghfirotin Marta ◽

Utami Tyas ◽

Cahyanto Muhammad Nur ◽

Widada Jaka ◽

Rahayu Endang Sutriswati

Keyword(s):

Placebo Group ◽

Gut Microbiota ◽

Lactobacillus Plantarum ◽

Coliform Bacteria ◽

School Age Children ◽

Controlled Study ◽

School Age ◽

Double Blind ◽

Probiotic Group ◽

E Coli

Consumption of probiotics is known to influence the gut microbiota. The aim of this study was to assess the effect of probiotic powder containing Lactobacillus plantarum Dad-13 on bacterial composition in the gut by examining fecal samples of school-age children in Yogyakarta, Indonesia. This is a randomized, double-blind, placebo-controlled study. A total of 40 healthy subjects were recruited for this study and were divided into two groups: placebo group and probiotic group. The placebo group consumed skim milk and the probiotic group consumed probiotic powder containing L. plantarum Dad-13 (2 × 109 CFU/g) for 65 days. The results showed that placebo intake had no significant effect on gut microbiota; however, probiotic caused a significant increase in L. plantarum and Lactobacillus population, while decreasing the population of E. coli and non-E. coli coliform bacteria by 55% and 75%, respectively and Bifidobacteria count did not change significantly. The study concluded that consumption of probiotic powder L. plantarum Dad-13 could increase propionic acid thereby decreasing the gut pH which has an effect on the microbial population.

Download Full-text