Oral supplementation with l-glutamine alters gut microbiota of obese and overweight adults: A pilot study

An alcohol-free beer including the substitution of regular carbohydrates for low doses of isomaltulose and maltodextrin within meals significantly impacts gut microbiota in diabetic subjects with overweight or obesity.

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Gut microbiota and fatigue in rectal cancer patients: a cross-sectional pilot study

Supportive Care in Cancer ◽

10.1007/s00520-021-06013-2 ◽

2021 ◽

Author(s):

Velda J. González-Mercado ◽

Jean Lim ◽

Sara Marrero ◽

Elsa Pedro ◽

Leorey N. Saligan

Keyword(s):

Rectal Cancer ◽

Pilot Study ◽

Gut Microbiota ◽

Cancer Patients ◽

Cross Sectional

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Impact of Hypocaloric Hyperproteic Diet on Gut Microbiota in Overweight or Obese Patients with Nonalcoholic Fatty Liver Disease: A Pilot Study

Digestive Diseases and Sciences ◽

10.1007/s10620-016-4179-1 ◽

2016 ◽

Vol 61 (9) ◽

pp. 2721-2731 ◽

Cited By ~ 28

Author(s):

Zoltan Pataky ◽

Laurence Genton ◽

Laurent Spahr ◽

Vladimir Lazarevic ◽

Sylvain Terraz ◽

...

Keyword(s):

Liver Disease ◽

Pilot Study ◽

Gut Microbiota ◽

Fatty Liver ◽

Nonalcoholic Fatty Liver Disease ◽

Fatty Liver Disease ◽

Obese Patients ◽

Nonalcoholic Fatty Liver

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Iron deficiency anemia-related gut microbiota dysbiosis in infants and young children: A pilot study

Acta Microbiologica et Immunologica Hungarica ◽

10.1556/030.65.2018.045 ◽

2018 ◽

Vol 65 (4) ◽

pp. 551-564 ◽

Cited By ~ 5

Author(s):

Audrone Muleviciene ◽

Federica D’Amico ◽

Silvia Turroni ◽

Marco Candela ◽

Augustina Jankauskiene

Keyword(s):

Pilot Study ◽

Iron Deficiency ◽

Young Children ◽

Gut Microbiota ◽

Iron Deficiency Anemia ◽

Deficiency Anemia ◽

Gut Microbiota Dysbiosis ◽

Infants And Young Children

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Encapsulated cyclosporine does not change the composition of the human microbiota when assessed ex vivo and in vivo

Journal of Medical Microbiology ◽

10.1099/jmm.0.001130 ◽

2020 ◽

Vol 69 (6) ◽

pp. 854-863

Author(s):

Catherine O'Reilly ◽

Órla O’Sullivan ◽

Paul D. Cotter ◽

Paula M. O’Connor ◽

Fergus Shanahan ◽

...

Keyword(s):

Pilot Study ◽

Gut Microbiota ◽

Targeted Delivery ◽

Healthy Subjects ◽

Ex Vivo ◽

16S Rrna Gene Sequencing ◽

Rrna Gene ◽

Microbiota Composition ◽

Faecal Samples

Introduction. Management of steroid-refractory ulcerative colitis has predominantly involved treatment with systemic cyclosporine A (CyA) and infliximab. Aim. The purpose of this study was to assess the effect of using a colon-targeted delivery system CyA formulation on the composition and functionality of the gut microbiota. Methodology. Ex vivo faecal fermentations from six healthy control subjects were treated with coated minispheres (SmPill) with (+) or without (−) CyA and compared with a non-treated control in a model colon system. In addition, the in vivo effect of the SmPill+CyA formulation was investigated by analysing the gut microbiota in faecal samples collected before the administration of SmPill+CyA and after 7 consecutive days of administration from eight healthy subjects who participated in a pilot study. Results. Analysis of faecal samples by 16S rRNA gene sequencing indicated little variation in the diversity or relative abundance of the microbiota composition before or after treatment with SmPill minispheres with or without CyA ex vivo or with CyA in vivo. Short-chain fatty acid profiles were evaluated using gas chromatography, showing an increase in the concentration of n-butyrate (P=0.02) and acetate (P=0.32) in the faecal fermented samples incubated in the presence of SmPill minispheres with or without CyA. This indicated that increased acetate and butyrate production was attributed to a component of the coated minispheres rather than an effect of CyA on the microbiota. Butyrate and acetate levels also increased significantly (P=0.05 for both) in the faecal samples of healthy individuals following 7 days’ treatment with SmPill+CyA in the pilot study. Conclusion. SmPill minispheres with or without CyA at the clinically relevant doses tested here have negligible direct effects on the gut microbiota composition. Butyrate and acetate production increased, however, in the presence of the beads in an ex vivo model system as well as in vivo in healthy subjects. Importantly, this study also demonstrates the relevance and value of using ex vivo colon models to predict the in vivo impact of colon-targeted drugs directly on the gut microbiota.

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Effect of Taro Starch, Beet Juice, Probiotic, and/or Psicose on Gut Microbiota in a Type 2 Diabetic Rat Model: A Pilot Study

Journal of Nutrition and Metabolism ◽

10.1155/2021/1825209 ◽

2021 ◽

Vol 2021 ◽

pp. 1-8

Author(s):

Ingrid S. Surono ◽

Ata Aditya Wardana ◽

Priyo Waspodo ◽

Budi Saksono ◽

Koen Venema

Keyword(s):

Pilot Study ◽

Gut Microbiota ◽

Functional Food ◽

Diabetic Rats ◽

Diabetic Rat ◽

Rrna Gene ◽

Modified Starch ◽

Food Ingredients ◽

Native Starch

Background and Objectives. The gut microbiota has been shown to be involved in the development and severity of type 2 diabetes (T2D). The aim of the present study was to test the effect of potential functional food ingredients, alone or in combination, on the gut microbiota composition in diabetic rats in a pilot study of 1 week of feeding. Methods. In a pilot study to modulate the composition of the gut microbiota, (i) native taro starch, (ii) modified taro starch, (iii) beet juice, (iv) psicose, (v) the probiotic L. plantarum IS-10506, (vi) native starch combined with beet juice, (vii) native starch to which beet juice was adsorbed, (viii) modified starch combined with beet juice, and (ix) modified starch to which beet juice was adsorbed were fed to rats in which T2D was induced with streptozotocin (STZ). After one week, the composition of the gut microbiota was evaluated by sequencing the PCR-amplified V3-V4 region of the 16S rRNA gene. Results and Conclusions. The next-generation sequencing showed that 13 microbial taxa of the gut microbiota were significantly different between groups, depending on the treatment. The results of this pilot study will be used to design a 4-week intervention study in STZ-induced T2D rats to determine the best functional food for counteracting T2D, including their effects on satiety hormones. This should ultimately lead to the development of functional foods for prediabetic and diabetic individuals.

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