scholarly journals Breeding technique of Selenothrips rubrocinctus (Giard, 1901) (Thysanoptera: Thripidae) on roses leaflets

2021 ◽  
Vol 3 ◽  
pp. ec03054
Author(s):  
Jailma R. Dos Santos ◽  
Brigida Souza ◽  
Marvin M. Pec Hernandez ◽  
Letícia G. de Souza ◽  
Luis Claudio P. Silveira
Keyword(s):  
Population Density ◽  
Filter Paper ◽  
Abiotic Factors ◽  
Petri Dish ◽  
Entire Period ◽  
Distilled Water ◽  
Breeding Technique ◽  
Microcentrifuge Tube ◽  
Paper Disk ◽  
Filter Paper Disk

The development of adequate methods for maintaining populations of arthropod organisms in the laboratory has been a challenge due to the characteristics of each species. This work has aimed to define a method for breeding Selenothrips rubrocinctus (Giard, 1901) in rose leaflets in order to study this species in the laboratory. A condition which could maintain the leaflets turgor for a longer time was sought, in order to guarantee both the survival and multiplication of the insects, and less influence of abiotic factors. Four types of substrates were tested: a) a filter paper disk moistened with distilled water covering the bottom of a Petri dish and; b) a vegetable sponge moistened with distilled water surrounding the base of the leaflet; c) a potato, dextrose and agar (BDA) in a microcentrifuge tube surrounding the base of the leaflet; and d) hydrogel in a microcentrifuge tube surrounding the base of the leaflet. The filter paper moistened with distilled water allowed 65% of the leaflets to remain turgid over a 10-day period and was the most suitable substrate for thrips breeding. With the results at hand, we described S. rubrocinctus breeding in the laboratory. The adopted methodology provided the population density stability of the bred insects, as well as the obtainment of specimens of S. rubrocinctus in quantity and quality throughout the entire period of development of studies on the biology of the species.

scholarly journals Paper-based electrochemical immunoassay for rapid, inexpensive cancer biomarker protein detection

2014 ◽  
Vol 6 (22) ◽  
pp. 8878-8881 ◽  
Author(s):  
C. K. Tang ◽  
A. Vaze ◽  
J. F. Rusling
Keyword(s):  
Prostate Specific Antigen ◽  
Filter Paper ◽  
Rapid Detection ◽  
Specific Antigen ◽  
Protein Detection ◽  
Cancer Biomarker ◽  
Electrochemical Immunoassay ◽  
Paper Disk ◽  
Filter Paper Disk

Inexpensive, reusable electrochemical chips were configured as immunosensors by using a filter paper disk equipped with antibodies. Rapid detection of cancer biomarker protein prostate specific antigen (PSA) in serum was achieved with 6 pg mL−1 detection in ∼15 min.

X-ray fluorescence determination of Ni, Ti and Nb in memory alloys using solution and thin film samples with an internal standard

2019 ◽  
Vol 11 (28) ◽  
pp. 3619-3622
Author(s):  
L. F. Tian ◽  
Y. C. Dai ◽  
D. S. Zou ◽  
T. C. Lei ◽  
S. K. Huang
Keyword(s):  
Thin Film ◽  
Filter Paper ◽  
Internal Standard ◽  
X Ray ◽  
Xrf Analysis ◽  
Fluorescence Determination ◽  
Paper Disk ◽  
Filter Paper Disk

Specimen for XRF analysis of high content of Ni, Ti and Nb in memory alloys, prepared by solution (a) and a filter paper disk (b).

scholarly journals Filter-Paper Disk Chromatography

Nature ◽  
1953 ◽  
Vol 171 (4340) ◽  
pp. 42-42 ◽  
Author(s):  
H. PROOM ◽  
A. J. WOIWOD
Keyword(s):  
Filter Paper ◽  
Paper Disk ◽  
Filter Paper Disk

scholarly journals Uji Antibakteri Fraksi Aktif Ekstrak Aseton Kulit Batang Shorea acuminatissima terhadap Staphylococcus aureus dan Pseudomonas aeruginosa Multiresisten antibiotik

2019 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Imam Prayitno ◽  
Haryoto Saroyobudiyon ◽  
Peni Indrayudha
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Filter Paper ◽  
Paper Disk ◽  
Filter Paper Disk

Penyakit infeksi merupakan masalah serius di Indonesia, termasuk di dalamnya resistensi mikroba terhadap obat-obatan yang ada. Salah satu tumbuhan hutan tropis Indonesia adalah Shorea accuminatissima yang termasuk dalam famili Dipterocarpaceae dan dilaporkan mempunyai kandungan senyawa fenolik dengan aktivitas biologi seperti antibakteri, antioksidan dan antifungi. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak aseton dan fraksi aktif ekstrak aseton terhadap S. aureus dan P. aeruginosa multiresisten antibiotik. Ekstrak aseton diperoleh dengan metode maserasi, selanjutnya diuji aktivitas antibakterinya. Ekstrak aseton difraksinasi menggunakan kromatografi cair vakum (KCV). Setiap fraksi diuji bioautografi untuk melihat aktivitas antibakterinya menggunakan metode filter paper disk dengan bakteri uji S. aureus dan P. aeruginosa. Fraksi dengan zona hambat terbesar merupakan fraksi aktif Kemudian dilakukan uji aktivitas antibakteri fraksi aktif ekstrak aseton. Kadar terkecil yang dapat menghambat pertumbuhan bakteri disebut sebagai kadar bunuh minimum (KBM).  Hasil penelitian menunjukkan ekstrak aseton kulit batang Shorea accuminatissima mempunyai nilai KBM 0,25% b/v terhadap S. aureus multiresisten antibiotik dan nilai KBM 1% b/v terhadap P. aeruginosa multiresisten antibiotik. Fraksi aktif adalah fraksi C. Fraksi aktif mempunyai nilai KBM 0,125% b/v terhadap S. aureus multiresisten antibiotik dan nilai KBM 0,25% b/v terhadap P. aeruginosa multiresisten antibiotik.

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