scholarly journals Multiplex PCR for detection of the predominant diarrheacausing protozoa (Giardia intestinalis, Entamoeba histolytica, and Cryptosporidium parvum) in fecal samples of cattle from Babylon Province, Iraq

2020 ◽  
Keyword(s):  
Multiplex Pcr ◽  
Entamoeba Histolytica ◽  
Cryptosporidium Parvum ◽  
Giardia Intestinalis ◽  
Fecal Samples

scholarly journals Simultaneous Detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in Fecal Samples by Using Multiplex Real-Time PCR

2004 ◽  
Vol 42 (3) ◽  
pp. 1220-1223 ◽  
Author(s):  
J. J. Verweij ◽  
R. A. Blange ◽  
K. Templeton ◽  
J. Schinkel ◽  
E. A. T. Brienen ◽  
...  
Keyword(s):  
Real Time ◽  
Entamoeba Histolytica ◽  
Cryptosporidium Parvum ◽  
Real Time Pcr ◽  
Giardia Lamblia ◽  
Simultaneous Detection ◽  
Fecal Samples

scholarly journals FREQUENCY OF ENTEROPARASITOSES IN PRESCHOOL CHILDREN ATTENDING DAYCARE CENTERS: A SURVEY APPLYING PARASITOLOGICAL AND IMMUNOLOGICAL METHODS

2019 ◽  
Vol 48 (2) ◽  
pp. 121-133
Author(s):  
Gabriela Cardoso Goes ◽  
Karina Costa Coelho Gonçalves ◽  
Adriana Pittella Sudré ◽  
Danuza Pinheiro Bastos Garcia Mattos ◽  
Beatriz Brener ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Entamoeba Histolytica ◽  
Intestinal Parasites ◽  
Parasite Infection ◽  
Giardia Intestinalis ◽  
Diagnostic Methods ◽  
Immunological Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fecal Samples ◽  
Daycare Centers

The present study evaluated the frequency of intestinal parasitoses in children in public day care centers applying parasitological and immunological diagnostic methods. Fecal samples from 121 children from six public daycare centers were analyzed using parasitological techniques. Epidemiological data were obtained through a questionnaire, where parents and / or guardians were asked, for instance, whether the children had contact with soil, ate raw food, such as vegetables or raw or undercooked meat, normally walked around barefoot or had contact with animals. Fecal samples from 82 children were also tested for Giardia intestinalis and Cryptosporidium sp. coproantigen using the enzyme-linked immunosorbent assay (ELISA) which was also used for Entamoeba coproantigen detection only in samples that tested positive for the parasite by parasitological stool exam/optical microscopy. Intestinal parasite infection was noted in 23.1% (28/121) of the children. The most frequent parasite was Giardia intestinalis (13.2%), followed by Entamoeba coli (5.8%), Blastocystis spp. (1.7%), Endolimax nana (1.7%), Enterobius vermicularis (1.7%), Cystoisospora belli (0.8%),Entamoeba histolytica/E. dispar complex (0.8%), and Ascaris lumbricoides (0.8%). Positivity for parasite infection using parasitological stool exams was significantly associated with age groups, with a higher frequency in 4 to 6 year old children (p=0.03). No association or significant variations were noted in the prevalence of intestinal parasites in relation to the epidemiological variables studied. All samples were negative for Cryptosporidium sp. and Entamoeba histolytica detected by immunological testing, and 17.1% (14/82) children tested positive for Giardia intestinalis, although using parasitological exam/optical microscopy, only 14.6% (12/82) tested positive. The high incidence of intestinal parasites, especially protozoans, suggests probable interpersonal transmission among the children, environmental contamination, or even contaminated food/water intake. Thus, consolidation of preventive measures and efficient diagnostic resources as well as control of intestinal parasites and patient treatment are of utmost importance.

Simultaneous detection and differentiation of Entamoeba histolytica , E. dispar , E. moshkovskii , Giardia lamblia and Cryptosporidium spp. in human fecal samples using multiplex PCR and qPCR-MCA

Acta Tropica ◽  
2016 ◽  
Vol 162 ◽  
pp. 233-238 ◽  
Author(s):  
Nozhat Zebardast ◽  
Farshid Yeganeh ◽  
Mohammad Javad Gharavi ◽  
Alireza Abadi ◽  
Seyyed Javad Seyyed Tabaei ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Simultaneous Detection ◽  
Fecal Samples ◽  
Cryptosporidium Spp

High‐Yield Purification of Giardia intestinalis Cysts from Fecal Samples

2020 ◽  
Vol 59 (1) ◽  
Author(s):  
Paul Ogbuigwe ◽  
Anthony B. Pita ◽  
Matthew A. Knox ◽  
Niluka Velathanthiri ◽  
David T. S. Hayman
Keyword(s):  
Giardia Intestinalis ◽  
High Yield ◽  
Fecal Samples

scholarly journals Diagnosis of Blastocystis sp., Cryptosporidium sp., and Giardia intestinalis by Multiplex PCR: An Optimization Study

2021 ◽  
Author(s):  
Ali Ahmet Kilimcioğlu ◽  
Nogay Girginkardeşler ◽  
Tuba Oyur ◽  
Selin Bölük Sabuncu ◽  
Didem Düzyol Azak ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Dna Isolation ◽  
Giardia Intestinalis ◽  
Molecular Method ◽  
Chain Reaction ◽  
Optimization Study ◽  
Optimized Protocol ◽  
Blastocystis Sp ◽  
Stool Samples ◽  
Polymerase Chain

Objective: It was aimed to develop a new Multiplex Polymerase Chain Reaction (PCR) protocol with isolates obtained from local patients for the diagnosis of Blastocystis sp., Cryptosporidium sp. and Giardia intestinalis, which can cause severe gastrointestinal system complaints especially in immunocompromised patients and children. Method: DNA isolation was performed with a commercial kit from three stool samples of different patients whose microscopic examination showed dense amounts of Blastocystis sp., Cryptosporidium sp. and Giardia intestinalis. First, a special PCR protocol has been developed for each protozoon. Then, the multiplex PCR protocol, in which these three protozoa can be diagnosed together, was optimized. Results: In the multiplex PCR protocol performed after DNA isolation, bands of 95 bp., 227 bp. and 258 bp. were obtained for Cryptosporidium sp., Blastocystis sp. and G. intestinalis, respectively. Conclusion: Blastocystis sp., Cryptosporidium sp. and Giardia intestinalis were diagnosed by multiplex PCR with the original protocol developed. Due to the difficulties in using different methods in parasitological examination, by adding other protozoa important for public health to this optimized protocol, it will be possible to detect a large number of parasites with a single molecular method.

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