The increase in online courses offered in higher education, the reliance on highly developed academic literacy skills to learn course content, the complex nature of media literacy, the negotiation of multiple technologies, and the corresponding media literacy together can be quite challenging for online learners. Most research conducted on academic literacies has focused primarily on academic reading and writing practices rather than on media literacy. This chapter discusses an investigation of media literacy in an online course, the experience learners had with this literacy and online tasks. The chapter discusses results of data from the online learners and instructor, which showed the instructor required different media literacy proficiency than what the online learners possessed prior to beginning the online course. Finally, the chapter presents implications the study findings have for online instructors' effective development, design, and delivery of online courses and development of online learners' media literacy.