The in vitro adrenal steroid secretion of wild Norway rats (Rattus norvegicus) captured during the winter differed in quantity, but not in quality, from that of similar rats captured during the summer. Whereas there was no evident seasonal effect on adrenal weight either in males or in females, adrenals of both sexes secreted at a faster rate during the winter than during the summer, this increase involving the four groups of steroids characterized chromatographically during both seasons and in both sexes; the predominant steroid secreted was corticosterone. This seasonal adjustment in adrenal activity is similar to that observed in white rats kept in group cages exposed outdoors to the natural summer and winter environmental conditions, but it differs from that found in white rats cold-acclimated in the laboratory, in which adrenal activity is lower than normal after cold acclimation.