COMPARATIVE ANALYSIS OF THE BINDING EFFICIENCY OF TUMOR- TARGETING PHAGE PARTICLES INTO CANCER CELLS AND HEALTH BRAIN CELLS

The binding efficiency of tumor-targeting phage particles, obtained by phage display, into human glioma cell line U-87 MG and health brain cells was evaluated by flow cytometry and enzyme-linked immunosorbent assay (ELISA). Based on the obtained data, tumor-targeting phage particles that provide the most efficient binding to human glioma cells U-87 MG in vitro are selected for further studies.

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ONCOLYTIC PROPERTIES OF SOME ORTHOPOXVIRUSES, ADENOVIRUSES AND PARVOVIRUSES IN HUMAN GLIOMA CELLS

Annals of the Russian academy of medical sciences ◽

10.15690/vramn.v68i12.853 ◽

2013 ◽

Vol 68 (12) ◽

pp. 4-8 ◽

Cited By ~ 2

Author(s):

I. A. Razumov ◽

V. A. Svyatchenko ◽

E. V. Protopopova ◽

G. V. Kochneva ◽

N. N. Kiselev ◽

...

Keyword(s):

Glioma Cell ◽

Glioma Cells ◽

Oncolytic Viruses ◽

Human Glioma ◽

Cell Models ◽

Human Glioma Cells ◽

Human Malignant Glioma ◽

Oncolytic Activity

Currently one of the most promising approaches in development of cancer virotherapy is based on the ability of oncolytic viruses to selective infection and lysis of tumor cells. Aim.The goal of the study was to identify and evaluate perspective oncolytic viruses capable of selectively destroying human glioma cells. Patients and methods. Original GB2m, GA14m and GB22m glioma cell cultures derived from patients were used for evaluating in vitro oncolytic activity of some typical orthopoxviruses, adenoviruses and parvoviruses. Results. The oncolytic activity in the human glioma cell models was confirmed for LIVP and WR strains of vaccinia virus, Adel2 and Ad2del strains with deletions within E1B/55K gene and derived from human adenoviruses type 2 and 5, respectively. Conclusions. We consider these oncolytic viruses as promising agents for the treatment of human malignant glioma.

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MicroRNA-585 inhibits human glioma cell proliferation by directly targeting MDM2

Cancer Cell International ◽

10.1186/s12935-020-01528-w ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Wangsheng Chen ◽

Lan Hong ◽

Changlong Hou ◽

Yibin Wang ◽

Fei Wang ◽

...

Keyword(s):

Cell Proliferation ◽

Cell Lines ◽

Glioma Cell ◽

Glioma Cells ◽

Human Glioma ◽

Human Glioma Cells ◽

Human Glioma Cell ◽

Glioma Cell Proliferation

Abstract Background MicroRNAs (miRNAs) are important regulators for cancer cell proliferation. miR-585 has been shown to inhibit the proliferation of several types of cancer, however, little is known about its role in human glioma cells. Methods miR-585 levels in human glioma clinical samples and cell lines were examined by quantitative real-time PCR (qRT-PCR) analysis. Cell proliferation was measured by Cell Counting Kit-8 (CCK-8) and EdU incorporation assays in vitro. For in vivo investigations, U251 cells were intracranially inoculated in BALB/c nude mice and xenografted tumors were visualized by magnetic resonance imaging (MRI). Results miR-585 expression is downregulated in human glioma tissues and cell lines compared with non-cancerous counterparts. Additionally, miR-585 overexpression inhibits and its knockdown promotes human glioma cell proliferation in vitro. Moreover, miR-585 overexpression also inhibits the growth of glioma xenografts in vivo, suggesting that miR-585 may act as a tumor suppressor to inhibit the proliferation of human glioma. Furthermore, miR-585 directly targets and decreases the expression of oncoprotein murine double minute 2 (MDM2). More importantly, the restoration of MDM2 via enforced overexpression markedly rescues miR-585 inhibitory effect on human glioma cell proliferation, thus demonstrating that targeting MDM2 is a critical mechanism by which miR-585 inhibits human glioma cell proliferation. Conclusions Our study unveils the anti-proliferative role of miR-585 in human glioma cells, and also implicates its potential application in clinical therapy.

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Comparative Analysis of the Binding Efficiency of Tumor-Targeting Phage Particles to Cancer Cells and Health Brain Cells

Biotekhnologiya ◽

10.21519/0234-2758-2020-36-6-61-67 ◽

2020 ◽

Vol 36 (6) ◽

pp. 61-67

Author(s):

A.A. Voitova ◽

M.D. Dmitrieva ◽

V.A. Richter ◽

E.V. Kuligina

Keyword(s):

Flow Cytometry ◽

Phage Display ◽

Tumor Targeting ◽

Specific Binding ◽

Microscopic Analysis ◽

Enzyme Linked Immunosorbent Assay ◽

Brain Cells ◽

Russian Science ◽

Core Facilities

The binding efficiency of tumor-targeting phage particles, which exhibited tumor-addressing peptides and were obtained by phage display, to the human glioblastma cell line U-87 MG and health brain cells DKM- 5 has been evaluated by flow cytometry and enzyme-linked immunosorbent assay (ELISA). The specific binding of the selected bacteriophages to U-87 MG cells was shown by fluorescence microscopy. Based on the obtained data, tumor-targeting phage particles were selected that provide the most efficient binding to the U-87 MG cells in vitro for further studies in order to create targeted antitumor compounds. phage display, tumor-addressing peptides, glioblastoma, flow cytometry, ELISA The authors are grateful to S. I. Baiborodin, Head of the Microscopic Analysis of Biological Objects Core Facilities Center, SB RAS, for assistance in conducting confocal microscopy. This study was supported by the Russian Science Fund (grant no. 19-44-02006).

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