DEVELOPMENT OF FLUORESCENCE POLARIZATION IMMUNOASSAY OF PHTHALATES: COMPARISON OF DIFFERENT DERIVATIVES IN OBTAINING ANTIBODIES AND TRACERS

2021 ◽  
Vol 1 (19) ◽  
pp. 232-234
Author(s):  
I.A. Shanin ◽  
M.R. Karimova ◽  
L.I. Mukhametova
Keyword(s):  
Water Samples ◽  
Fluorescence Polarization ◽  
Dibutyl Phthalate ◽  
Polyclonal Antibodies ◽  
Fluorescence Polarization Immunoassay ◽  
Protein Conjugates ◽  
Portable Detector

Reagents for fluorescence polarization immunoassay (FPIA) of phthalates have been obtained: phthalate-protein conjugates, polyclonal antibodies, tracers - antigens labeled with fluorescein derivatives. By the example of dibutyl phthalate (DBP) with the use of a portable detector Sentry-200, the FPIA method was developed, which makes it possible to determine DBP in water samples in concentrations 1 μg / ml in 5 min.

scholarly journals Fluorescence Polarization Immunoassay for Determination of Enrofloxacin in Pork Liver and Chicken

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4462
Author(s):  
Xing Shen ◽  
Jiahong Chen ◽  
Shuwei Lv ◽  
Xiulan Sun ◽  
Boris B. Dzantiev ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Limit Of Detection ◽  
Polyclonal Antibodies ◽  
Screening Method ◽  
Sample Pretreatment ◽  
High Sensitivity ◽  
Cross Reactivity ◽  
Fluorescence Polarization Immunoassay ◽  
Pork Liver ◽  
Sensitivity Specificity

Enrofloxacin (ENR) is a widely used fluoroquinolone (FQ) antibiotic for antibacterial treatment of edible animal. In this study, a rapid and highly specific fluorescence polarization immunoassay (FPIA) was developed for monitoring ENR residues in animal foods. First, ENR was covalently coupled to bovine serum albumin (BSA) to produce specific polyclonal antibodies (pAbs). Three fluorescein-labeled ENR tracers (A, B, and C) with different spacers were synthesized and compared to obtain higher sensitivity. Tracer C with the longest arm showed the best sensitivity among the three tracers. The developed FPIA method showed an IC50 (50% inhibitory concentration) of 21.49 ng·mL−1 with a dynamic working range (IC20–IC80) of 4.30–107.46 ng·mL−1 and a limit of detection (LOD, IC10) of 1.68 ng·mL−1. The cross-reactivity (CR) of several structurally related compounds was less than 2%. The recoveries of spiked pork liver and chicken samples varied from 91.3% to 112.9%, and the average coefficients of variation were less than 3.83% and 5.13%, respectively. The immunoassay took only 8 min excluding sample pretreatment. This indicated that the established method had high sensitivity, specificity, and the advantages of simplicity. Therefore, the proposed FPIA provided a useful screening method for the rapid detection of ENR residues in pork liver and chicken.

Production of Polyclonal Antibodies and Development of Fluorescence Polarization Immunoassay for Sulfanilamide

2005 ◽  
Vol 38 (6) ◽  
pp. 951-969 ◽  
Author(s):  
S. A. Eremin ◽  
N. R. Murtazina ◽  
D. N. Ermolenko ◽  
A. V. Zherdev ◽  
A. A. Mart'ianov ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Polyclonal Antibodies ◽  
Fluorescence Polarization Immunoassay

Express detection of nonylphenol in water samples by fluorescence polarization immunoassay

2003 ◽  
Vol 378 (3) ◽  
pp. 634-641 ◽  
Author(s):  
Julia N. Yakovleva ◽  
Anna Yu. Lobanova ◽  
Ekaterina A. Shutaleva ◽  
Maria A. Kourkina ◽  
Andrey A. Mart’ianov ◽  
...  
Keyword(s):  
Water Samples ◽  
Fluorescence Polarization ◽  
Fluorescence Polarization Immunoassay

A fluorescence polarization immunoassay method for detection of the bisphenol A residue in environmental water samples based on a monoclonal antibody and 4′-(aminomethyl)fluorescein

2015 ◽  
Vol 7 (10) ◽  
pp. 4246-4251 ◽  
Author(s):  
Pinong Huang ◽  
Suqing Zhao ◽  
Sergei A. Eremin ◽  
Shengwu Zheng ◽  
Dan Lai ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Bisphenol A ◽  
Water Samples ◽  
Fluorescence Polarization ◽  
Environmental Water ◽  
Environmental Water Samples ◽  
Fluorescence Polarization Immunoassay

A homogeneous fluorescence polarization immunoassay (FPIA) was developed to detect bisphenol A in environmental water samples.

scholarly journals A Modified Fluorescence Polarization Immunoassay Method Incorporating Fat Emulsion(FE-FPIA) to Determine Cyclosporin A Concentrations in Rat Skin.

1999 ◽  
Vol 22 (5) ◽  
pp. 539-542
Author(s):  
Yuji KUROSAKI ◽  
Tomoya ASADA ◽  
Nanae YASUMOTO ◽  
Toshiaki NAKAMURA ◽  
Mikio MASADA ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
Fluorescence Polarization ◽  
Fluorescence Polarization Immunoassay ◽  
Rat Skin ◽  
Fat Emulsion
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