Effects of gibberellic acid and naphthalene acetic acid on saffron plant (Crocus sativus L.) under field conditions

The present investigation was carried out on 12-year old trees of nectarine (Prunus persica (L.) Batsch var. nucipersica) cultivar May Fire raised on wild peach seedling rootstocks, Farmer’s orchard at Kotla- Barog in District Sirmour during the years 2014 and 2015. In this experiment, experimental trees were subjected to seventeen different treatments;T1: Pruning to retain 60 fruiting shoots tree-1 (control), T2: Pruning to retain 50 fruiting shoots tree-1, T3: Pruning to retain 40 fruiting shoots tree-1 ,T4: NAA 40 ppm, 2 WAPF (week after petal fall) ,T5: Ethrel 300 ppm, 2 WAPF, T6: GA3 100 ppm, 4 WAPF, T7: GA3 200 ppm, 4WAPF, T8: GA3 100 ppm, 6 WAPF T9: GA3 200 ppm, 6 WAPF, T10: Pruning to retain 50 fruiting shoots tree-1 + NAA 40 ppm (T4), T11: Pruning to retain 50 fruiting shoots tree-1 + Ethrel 300ppm (T5), T12: Pruning to retain 50 fruiting shoots tree-1 + GA3100 ppm (T6), T13: Pruning to retain 50 fruiting shoots tree-1 + GA3 100 ppm (T8),T14: Pruning to retain 40 fruiting shoots tree-1 + NAA 40 ppm (T4), T15: Pruning to retain 40 fruiting shoots tree -1 +Ethrel 300 ppm (T5),T16: Pruning to retain 40 fruiting shoots tree -1 + GA3 100 ppm (T6), T17: Pruning to retain 40 fruiting shoots tree -1 + GA3 100 ppm (T8), pertaining to alternative approach to chemical thinning, chemical thinner were more effective in improving the production of superior grade fruits than reducing the crop load directly by pruning or indirectly by GA 3 (Gibberellic Acid) treatments. Treatments with NAA (naphthalene acetic acid) at 40 ppm when applied two weeks after petal fall reduced the crop load to the greatest extent and improve the yield of superior grade fruits and increase the leaf to fruit ratio and decrease the fruit drop. Pruning to retain 40 fruiting shoots tree-1 + NAA 40 ppm, 2WAPF increased the shoot growth (160.80, 170.20cm), tree height (3.70,4.50 cm), tree spread (2.84,3.60), leaf area (42.71, 40.63 cm) during both the year. However, effect of chemical were less pronounced on trees subjected to severe pruning.

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In Vitro Shoot Regeneration and Development of Microcorms of Moroccan Saffron (Crocus sativus L.)

Atlas Journal of Plant Biology ◽

10.5147/ajpb.v0i0.113 ◽

2017 ◽

pp. 50-55

Author(s):

Khalid Lagram ◽

Mohamed Ben El Caid ◽

Souad El Aaouam ◽

Mohamed Lachheb ◽

Abdelhamid El Mousadik ◽

...

Keyword(s):

In Vitro Culture ◽

Culture Medium ◽

Crocus Sativus ◽

Induction Medium ◽

Naphthalene Acetic Acid ◽

Ms Medium ◽

Male Sterile ◽

Indirect Organogenesis ◽

Crocus Sativus L

Crocus sativus L. is a male sterile vegetatively propagated plant. Its flower produces stigmas that when dried, constitute the source of a spice commonly known as Saffron. Slow vegetative propagation and diseases limit the production and the development of saffron. “In vitro” culture could be an effective method to overcome these limitations by improving the quantity and the quality of the planting materials. In this work, Crocus sativus L. segments corms of cultivar from the region of Taliouine (Southeast of Morocco) were used for the propagation through indirect organogenesis. To optimize the in vitro growth conditions, we have used the Murashige and Skoog medium (MS medium), supplemented with 2.4-dichlorophenoxyacetic acid (2.4-D) and with 6-benzylaminopurin (BAP) at combination of various concentrations. Our results showed the formation of callus in 85.42% of explants that grow in a culture medium supplemented with 2,4-D combined with BAP, at a concentration of 1mg/l each. In addition, we observed that increasing the concentration of BAP in the culture medium to 1.5mg/l improved the rate of shoots initiation (0.81). In the meantime, we noted that a combination of BAP (8mg/l) and Naphthalene acetic acid (NAA; 2mg/l) has significantly improved the rate of the formation of advanced shoots (6.65). Finally, the shoots that developed were transferred to an induction medium of roots and corms. As a result, we observed that 50% of shoots tested in ½ MS medium supplemented with 2.4-D and of BAP (1 mg/l each) and 5% sucrose, formed corms. Our study provides a first database for in vitro culture of Moroccan saffron cultivars.

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Epigenetic stability in Saffron (Crocus sativus L.) accessions during four consecutive years of cultivation and vegetative propagation under open field conditions

Plant Science ◽

10.1016/j.plantsci.2018.09.005 ◽

2018 ◽

Vol 277 ◽

pp. 1-10 ◽

Cited By ~ 8

Author(s):

Matteo Busconi ◽

Giovanna Soffritti ◽

Lorenzo Stagnati ◽

Adriano Marocco ◽

Javier Marcos Martínez ◽

...

Keyword(s):

Open Field ◽

Vegetative Propagation ◽

Field Conditions ◽

Crocus Sativus ◽

Crocus Sativus L

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GROWTH, YIELD AND POSTHARVEST QUALITY OF WAX APPLE AS AFFECTED BY NAPHTHALENE ACETIC ACID APPLICATION

Revista Brasileira de Fruticultura ◽

10.1590/0100-2945-062/14 ◽

2015 ◽

Vol 37 (2) ◽

pp. 410-422

Author(s):

MOHAMMAD MONERUZZAMAN KHANDAKER ◽

ABM SHARIF HOSSAIN ◽

NORMANIZA OSMAN ◽

NASHRIYAH MAT ◽

AMRU NASRULHAQ BOYCE

Keyword(s):

Acetic Acid ◽

Fruit Size ◽

Growth Yield ◽

Fruit Growth ◽

Field Conditions ◽

Soluble Solids ◽

Growth And Yield ◽

Postharvest Quality ◽

Naphthalene Acetic Acid

Results of this study represent the first report of the effect of Naphthalene Acetic Acid (NAA) on the pre and post harvest quality of wax apple fruit. The wax apple trees were spray treated with 0, 5, 10 and 20 mg L-1 NAA under field conditions during 2008 to 2011. The experiments were carried out in Completely Randomized Design (CRD) with six replications. Leaf chlorophyll content, chlorophyll fluorescence, photosynthetic yield, net photosynthetic rate, drymatter content of leaves and total soluble solids and K+content of wax apple fruits were significantly increased after treatments with 10 mg L-1. Polygalacturonase activity significantly decreased with NAA treatments. The application of 5 mg L-1 NAA increased 27% more bud and reduced 42% less fruit drop compared to the control. In addition, higher protein and phosphate synthase activity of leaves, fruit set, fruit growth, larger fruit size and yield were recorded in NAA treated plants. In storage, treated fruits exhibited higher TSS and firmness and less weight loss, browning, titratable acidity, respiration and ethylene production than the control. It is concluded that spraying with 5 and 10 mg L-1 NAA once a week under field conditions produced better fruit growth and yield of the wax apple and maintained better fruit quality in postharvest storage.

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Effect of Gibberellic acid (GA3) and α-naphthalene acetic acid (NAA) on the growth of unproductive tillers and the grain yield of rice (Oryza sativa L.)

African Journal of Agricultural Research ◽

10.5897/ajar11.735 ◽

2012 ◽

Vol 7 (4) ◽

Author(s):

Yang Liu

Keyword(s):

Acetic Acid ◽

Oryza Sativa ◽

Gibberellic Acid ◽

Grain Yield ◽

Oryza Sativa L ◽

Naphthalene Acetic Acid

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In vitro propagation of the endangered orchid Dendrobium chryseum Rolfefrom protocorms culture

Nepal Journal of Science and Technology ◽

10.3126/njst.v19i1.29737 ◽

2020 ◽

Vol 19 (1) ◽

pp. 39-47 ◽

Cited By ~ 2

Author(s):

Sabitri Maharjan ◽

Laxmi Sen Thakuri ◽

Bir Bahadur Thapa ◽

Shreeti Pradhan ◽

Krishna K. Pant ◽

...

Keyword(s):

Acetic Acid ◽

Gibberellic Acid ◽

In Vitro Propagation ◽

Seedling Development ◽

Naphthalene Acetic Acid ◽

Mass Propagation ◽

Endangered Orchid ◽

Immature Seeds ◽

Indole 3 Acetic Acid

The immature seeds of Dendrobiumchryseum, asympodial epiphytic orchid with yellow flowers, were cultured in vitro, and the resultant protocorms were used as explants for seedling development. Protocorms were cultured on½ M.S. medium fortified with Kinetin (Kn), 6-Benzylaminopurine (BAP), and Gibberellic Acid (GA3) in three concentrations (0.5mg/l, 1.0mg/land 2.0 mg/l) both alone and supplemented with 5% and 10% coconut water (C.W.). The highest number of shootsofD. chryseum developed on ½ - M.S. medium fortified with 2.0mg/lofKn and10% C.W. and the longest shootsdeveloped on ½ M.S. media fortified with 1.0mg/lGA3, and 10% C.W. The shoot derived from protocorms were placed in ½ M.S. medium fortified with three different rooting hormones, Indole -3- acetic acid (IAA), Indole -3-butyric acid (IBA) and α-Naphthalene acetic acid (NAA) in different concentrations alone as well as with each 1.0mg/l hormone combined with 10% C.W. The most effective of these media was ½ M.S. medium fortified with 1.5 mg/l IAA for rooting as well as for the production of longest roots. The present study could be useful for standardizing the protocol for mass propagation of the endangered orchid Dendrobiumchryseum.

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