Studies of Solid Phase Immunoassay Based on Near Infrared Upconversion Nanoprobe

2018 ◽  
Vol 39 (8) ◽  
pp. 1059-1065
Author(s):  
周进 ZHOU Jin ◽  
张美玲 ZHANG Mei-ling ◽  
张俐 ZHANG Li ◽  
李翠霞 LI Cui-xia ◽  
赵慧颖 ZHAO Hui-ying ◽  
...  
Keyword(s):  
Near Infrared ◽  
Solid Phase ◽  
Solid Phase Immunoassay

Instrument To Detect Near-Infrared Fluorescence in Solid-Phase Immunoassay

1994 ◽  
Vol 66 (19) ◽  
pp. 3102-3107 ◽  
Author(s):  
Richard J. Williams ◽  
Narasimhachari. Narayanan ◽  
Guillermo A. Casay ◽  
Malgorzata. Lipowska ◽  
Jose Mauro. Peralta ◽  
...  
Keyword(s):  
Near Infrared ◽  
Solid Phase ◽  
Near Infrared Fluorescence ◽  
Solid Phase Immunoassay

Near-Infrared Heptamethine Cyanine Dyes: A New Tracer for Solid-Phase Immunoassays

1997 ◽  
Vol 51 (6) ◽  
pp. 836-843 ◽  
Author(s):  
Richard J. Williams ◽  
Jose Mauro Peralta ◽  
Victor C. W. Tsang ◽  
Narasimhachari Narayanan ◽  
Guillermo A. Casay ◽  
...  
Keyword(s):  
Spectral Properties ◽  
Near Infrared ◽  
Solid Phase ◽  
Human Immunoglobulin ◽  
Minimal Amount ◽  
Human Antibody ◽  
Near Ir ◽  
Solid Phase Immunoassay ◽  
Bioanalytical Applications ◽  
Aqueous Conditions

Near-infrared (near-IR) fluorescence has been used to develop a solid-phase immunoassay that detects trace amounts of human immunoglobulin (HuIgG). Various concentrations of HuIgG bound to a nitrocellulose surface were determined from the fluorescence generated by near-IR labeled goat anti-human antibody (GAHG) bound to the HuIgG. The GAHG was labeled with a heptamethine cyanine fluorophore that has spectral properties in the near-IR region (above 780 nm). These fluorophores are versatile because they can be modified for several bioanalytical applications. Fluorescence was detected with a near-IR fluorescence instrument previously developed in the laboratory. Two cyanine fluorophore labels were evaluated for the ability to selectively bind to GAHG on a nitrocellulose matrix with a minimal amount of background interference. After the most appropriate near-IR fluorophore was selected, the labeling of GAHG was optimized under aqueous conditions. The most effective GAHG–dye conjugates were used to develop an immunoassay to detect various concentrations of HuIgG. The results are presented, here. Solutions of HuIgG with concentrations as low as 10−10 molar have been detected with a minimum of interference.

Performance Evaluation of the Phosphorescent Porphyrin Label:  Solid-Phase Immunoassay of α-Fetoprotein

2002 ◽  
Vol 74 (22) ◽  
pp. 5845-5850 ◽  
Author(s):  
Tomás C. O'Riordan ◽  
Aleksi E. Soini ◽  
Dmitri B. Papkovsky
Keyword(s):  
Performance Evaluation ◽  
Solid Phase ◽  
Solid Phase Immunoassay

A solid-phase immunoassay of protease-resistant prion protein with filtration blotting involving sodium dodecyl sulfate

2006 ◽  
Vol 349 (2) ◽  
pp. 218-228 ◽  
Author(s):  
Yoshiteru Kobayashi ◽  
Naoyuki Kohno ◽  
Shoko Wanibe ◽  
Kazunari Hirayasu ◽  
Hitoshi Uemori ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Prion Protein ◽  
Solid Phase ◽  
Sodium Dodecyl ◽  
Solid Phase Immunoassay ◽  
Dodecyl Sulfate

scholarly journals Synthesis of Ge1−xSnx Alloy Thin Films by Rapid Thermal Annealing of Sputtered Ge/Sn/Ge Layers on Si Substrates

Materials ◽  
2018 ◽  
Vol 11 (11) ◽  
pp. 2248 ◽  
Author(s):  
Hadi Mahmodi ◽  
Md Hashim ◽  
Tetsuo Soga ◽  
Salman Alrokayan ◽  
Haseeb Khan ◽  
...  
Keyword(s):  
Thermal Annealing ◽  
Rapid Thermal Annealing ◽  
Near Infrared ◽  
Annealing Temperature ◽  
Solid Phase ◽  
Infrared Region ◽  
Alloy Formation ◽  
Phase Mixing ◽  
Si Substrates ◽  
Msm Photodetector

In this work, nanocrystalline Ge1−xSnx alloy formation from a rapid thermal annealed Ge/Sn/Ge multilayer has been presented. The multilayer was magnetron sputtered onto the Silicon substrate. This was followed by annealing the layers by rapid thermal annealing, at temperatures of 300 °C, 350 °C, 400 °C, and 450 °C, for 10 s. Then, the effect of thermal annealing on the morphological, structural, and optical characteristics of the synthesized Ge1−xSnx alloys were investigated. The nanocrystalline Ge1−xSnx formation was revealed by high-resolution X-ray diffraction (HR-XRD) measurements, which showed the orientation of (111). Raman results showed that phonon intensities of the Ge-Ge vibrations were improved with an increase in the annealing temperature. The results evidently showed that raising the annealing temperature led to improvements in the crystalline quality of the layers. It was demonstrated that Ge-Sn solid-phase mixing had occurred at a low temperature of 400 °C, which led to the creation of a Ge1−xSnx alloy. In addition, spectral photo-responsivity of a fabricated Ge1−xSnx metal-semiconductor-metal (MSM) photodetector exhibited its extending wavelength into the near-infrared region (820 nm).

Quantification of Lupus Anticoagulants in Clinical Samples Using Anti- β2GP1 and Anti-Prothrombin Monoclonal Antibodies

2001 ◽  
Vol 86 (08) ◽  
pp. 584-589 ◽  
Author(s):  
A. Le Querrec ◽  
J. Arnout ◽  
D. Arnoux ◽  
J. Y. Borg ◽  
C. Caron ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Lupus Anticoagulant ◽  
Solid Phase ◽  
Clinical Samples ◽  
Lupus Anticoagulants ◽  
Asymptomatic Patients ◽  
Solid Phase Immunoassay ◽  
Dependent Activity ◽  
Variable Contribution ◽  
Suitable Standard

SummaryQuantification of lupus anticoagulant (LA) in clinical samples is hampered by the lack of a suitable standard of activity. We evaluated the use of mAbs displaying LA activity for this purpose. As most patient samples contain both β2Glycoprotein I (β2GP1) and prothrombin dependent LA, a combination of two mAbs, one of each specificity, was added to normal plasma in a concentration from 0 to 60 g/ml. Eight assay systems using different reagents and instruments were used. The calibration curves were linear for all but one, with marked differences between the responsiveness to each mAb. A panel of plasmas from 69 patients with persistent LA diagnosed using the SSCISTH criteria was tested. An antiphospholipid syndrome (APS) was present in 40, whereas 29 were asymptomatic. LA activities of individual plasmas varied between assays (p <10–4), but homogeneous subgroups were identified. In a majority of samples, LA activity displayed a prothrombin-dependent profile, with a variable contribution of β2GP1-dependent activity. The latter was associated to β2GP1 antibodies detected by solid-phase immunoassay. By using 3 dilute Russell viper venom time assays, higher LA titers were found in APS, compared to asymptomatic patients (p <0.05).

scholarly journals IMMUNOLOGICAL IMBALANCE IN BREAST CANCER AND LUNG CANCER IN POSTMENOPAUSAL WOMEN

2018 ◽  
Vol 20 (6) ◽  
pp. 927-934 ◽  
Author(s):  
A. N. Glushkov ◽  
E. G. Polenok ◽  
L. A. Gordeeva ◽  
S. A. Mun ◽  
M. V. Kostyanko ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lung Cancer ◽  
Postmenopausal Women ◽  
Marked Effect ◽  
Solid Phase ◽  
Anticancer Effect ◽  
Class A ◽  
Solid Phase Immunoassay ◽  
The One

Previous studies reported some associations between class A antibodies specific for benzo[a]pyrene (IgA-Bp), estradiol (IgA-Es) and progesterone (IgA-Pg) and breast cancer (BC) in women like as with lung cancer (LC) in men. It was suggested that IgA-Bp and IgA-Es may stimulate tumor initiation and promotion, whereas IgA-Pg may inhibit the in vivo human carcinogenesis.The purpose of this study was to identify the suggested associations of such immunological imbalance with BC and LC in postmenopausal women.The serum A-class antibodies specific to benzo[a]pyrene, estradiol and progesterone (IgA-Bp, IgA-Es, IgA- Pg) were studied in 335 healthy women, 824 breast cancer (BC) patients and 127 cases of lung cancer (LC) by means of non-competitive solid phase immunoassay. The following results were obtained: Increased ratio of IgA-Bp and IgA-Es amounts exceeding the IgA-Pg levels was associated with a higher risk of breast cancer (OR = 2.8 and 2.4 respectively, p < 0.0001), and higher risk of LC (OR = 2.9 and 2.8, respectively, p < 0.0001). Conversely, the OR values decreased to 0.3-0.4 for BC and LC if IgA-Pg levels were higher than IgA-Bp and IgA-Es levels (p < 0.0001). These findings confirm the hypothesis that IgA-Bp and IgA-Es are capable to stimulate, and IgA-Pg, to inhibit the BC and LC occurrence n postmenopausal women. The balance between IgA-Bp and IgA-Es, on the one hand, and IgA-Pg, on the other hand, is much more important than individual contents of these antibodies.In conclusion, the phenomenon of “immunological interference” is revealed, i.e., the mutual enhancement of IgA-Bp and IgA-Es effects, thus, probably, stimulating the initial and subsequent events of carcinogenesis initiation and promotion, with a weak anticancer effect of IgA-Pg, and by weakening the mutual procarcinogenic effects of IgA-Bp and IgA-Es by the marked effect of IgA-Pg.

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