SCREENING OF COLLECTION STRAINS (COLLECTIONS) OF ENTOMOPATHOGENIC FUNGI AGAINST ASIAN LOCUSTS FOR SIGNS OF VIRULENCE

Habarshy ◽  
2021 ◽  
pp. 124-135
Author(s):  
U.T. Zhumatayeva ◽  
B.A. Duisembekov ◽  
A.M. Begalieva ◽  
U.O. Sabdenova ◽  
K.K. Yerkekulova
Keyword(s):  
Biological Activity ◽  
Mortality Rate ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Host Organism ◽  
High Biological Activity ◽  
Preliminary Screening ◽  
Test Organisms ◽  
Control Variant

The article provides a preliminary screening of Asian locust larvae aged 2-3 years for the virulence of 25 isolates isolated from insects belonging to different systematic groups of fungi Beauveria bassiana. Five of the 25 strains showed high biological activity against larvae, (BCo1-14, BSc1-15, BSc2-15, BTr1-16, BPit-16) reaching a mortality rate of 90-100% 21 days after inoculation. The strains that have the best effect on the level and rate of mortality of the host organism are - BCo1-14, BSc1-15, BSc2-15, BTr1-16, BPit-16. The greatest biological activity was observed in the BSc1-15 strain, as the mortality rate of test organisms was only 100% within 15 days of exposure. The lowest activity was shown by the BCi4-14 strain in the range of 35.0-62.5%. At the same time, the mortality rate of Asian locust larvae in the control variant (without treatment) for 21 days was 15.0±2.88%. Thus, the proportion of highly toxic forms (80-100% lethality) in the total number of studied strains was not more than 44%, and the proportion of mildly toxic forms (less than 80% activity) was 56%.

scholarly journals Evaluating the virulence of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) and Beauveria bassiana (Ascomycota: Hypocreales) isolates to Arabian rhinoceros beetle, Oryctes agamemnon arabicus

2015 ◽  
Vol 47 (3) ◽  
pp. 117 ◽  
Author(s):  
M.W. Khudhair ◽  
M.Z. Khalaf ◽  
H.F. Alrubeai ◽  
A.K. Shbar ◽  
B.S. Hamad ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Pupal Stage ◽  
Control Treatment ◽  
Rhinoceros Beetle ◽  
Oryctes Agamemnon ◽  
Lethal Time ◽  
Short Time

Virulence of entomopathogenic fungi <em>Metarhizium anisopliae</em> and <em>Beauveria bassiana</em> were tested against Arabian Rhinoceros Beetle, <em>Oryctes agamemnon arabicus</em> larvae. Four concentrations (1×10<sup>5</sup>, 1×10<sup>7</sup>, 1×10<sup>9</sup> and 1×10<sup>11</sup> conidia/mL<sup>–1</sup>) of two locally isolated entomopathogenic fungi spore suspensions were used in this study via larval direct spraying. Results revealed that both isolates can cause high mortality rate reaching 100% after 29 days. However, <em>Beauveria bassiana</em> scored higher mortality rate in short time especially at the concentration of 1×10<sup>11</sup> conidia/ mL<sup>–1</sup> with lethal time (LT)<sub>50</sub> 12.75 and LT<sub>90</sub> 20.00; while, <em>Metarhizium anisopliae</em> caused the higher percentage of malformed adults. Moreover, both isolates affected insect’s life cycle particularly in the pupal stage which was reduced remarkably by almost 50% in comparison with the control treatment.

scholarly journals Registration of entomopatogenic micromycete Cordyceps sp. in bloodsucking blackflies Wilhelmia equine (Linnaeus, 1758)

2020 ◽  
Vol 65 (1) ◽  
pp. 43-49
Author(s):  
D. V. Dovnar ◽  
E. N. Yankovskaya ◽  
D. V. Voitka ◽  
O. Yu. Baranov ◽  
V. M. Kaplich ◽  
...  
Keyword(s):  
Biological Activity ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Comparative Evaluation ◽  
Molecular Genetic ◽  
Biological Efficiency ◽  
In Vitro Experiments ◽  
Test Objects ◽  
Significant Mortality

An entomopathogenic fungi belonging to the genus Cordyceps was isolated from the affected larvae of the bloodsucking blackflies Wilhelmia equina (Linnaeus, 1758). Larval stage of Simulium were collected on the territory of the Lepel district of the Vitebsk region in the river Essa in July 2018. Isolate was identified using cultural-morphological and molecular-genetic methods.In vitro experiments comparative evaluation of biological activity of isolated strain Cordyceps sp. and Beauveria bassiana 10-06 was carried out for 3rd instar larvae of W. equina. Two different concentrations 1∙106 and 1∙107 conidia/ml were made and tested in 3 replications. On the 3rd day of incubation both fungi caused a significant mortality of test-objects (p = 0.03) compared to control. The biological efficiency of the concentration of 107 соnidia/ml of the strain Beauveria bassiana 10-06 on the 3rd day was 94.4 %, on the 4th – 98.3 %, of the strain Cordyceps sp. – 76.0 and 88.9 % respectively.

scholarly journals Entomopathogenic Fungi and Bacteria in a Veterinary Perspective

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 479
Author(s):  
Valentina Virginia Ebani ◽  
Francesca Mancianti
Keyword(s):  
Biological Activity ◽  
Bacillus Thuringiensis ◽  
Human Health ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Field Application ◽  
Farm Animals ◽  
Dermanyssus Gallinae ◽  
Alternative Drugs

The present study aimed to review the papers dealing with the biological activity of fungi and bacteria against some mites and ticks of veterinary interest. In particular, the attention was turned to the research regarding acarid species, Dermanyssus gallinae and Psoroptes sp., which are the cause of severe threat in farm animals and, regarding ticks, also pets. Their impact on animal and human health has been stressed, examining the weaknesses and strengths of conventional treatments. Bacillus thuringiensis, Beauveria bassiana and Metarhizium anisopliae are the most widely employed agents. Their activities have been reviewed, considering the feasibility of an in-field application and the effectiveness of the administration alone or combined with conventional and alternative drugs is reported.

scholarly journals BIOLOGICAL ACTIVITY OF SELECTED STRAINS OF ENTOMOPATHOGENIC FUNGI BEAUVERIA BASSIANA AGAINST LARVAE OF GALLERIA MILLONELLA L.

REPORTS ◽  
2021 ◽  
Vol 4 (338) ◽  
pp. 43-49
Author(s):  
U.T. Zhumatayeva ◽  
B.A Duisembekov ◽  
Kh.K. Kidirbaeva ◽  
G.A. Absattar
Keyword(s):  
Biological Activity ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi

A FACILE SYNTHESIS AND REACTIONS OF AMINO SELENOLO[2,3-b]PYRIDINE CARBOXYLATE

2015 ◽  
Vol 12 (1) ◽  
pp. 3910-3918 ◽  
Author(s):  
Dr Remon M Zaki ◽  
Prof Adel M. Kamal El-Dean ◽  
Dr Nermin A Marzouk ◽  
Prof Jehan A Micky ◽  
Mrs Rasha H Ahmed
Keyword(s):  
Biological Activity ◽  
Carbonyl Compounds ◽  
Mass Spectra ◽  
The Other ◽  
Pyridine Derivatives ◽  
Facile Synthesis ◽  
High Biological Activity ◽  
Basic Hydrolysis ◽  
Pyridine Carboxylate ◽  
Hydrolysis Of

 Incorporating selenium metal bonded to the pyridine nucleus was achieved by the reaction of selenium metal with 2-chloropyridine carbonitrile 1 in the presence of sodium borohydride as reducing agent. The resulting non isolated selanyl sodium salt was subjected to react with various α-halogenated carbonyl compounds to afford the selenyl pyridine derivatives 3a-f  which compounds 3a-d underwent Thorpe-Ziegler cyclization to give 1-amino-2-substitutedselenolo[2,3-b]pyridine compounds 4a-d, while the other compounds 3e,f failed to be cyclized. Basic hydrolysis of amino selenolo[2,3-b]pyridine carboxylate 4a followed by decarboxylation furnished the corresponding amino selenolopyridine compound 6 which was used as a versatile precursor for synthesis of other heterocyclic compound 7-16. All the newly synthesized compounds were established by elemental and spectral analysis (IR, 1H NMR) in addition to mass spectra for some of them hoping these compounds afforded high biological activity.

scholarly journals Effect of the native strain of the predator Nesidiocoris tenuis Reuter and the entomopathogenic fungi Beauveria bassiana and Lecanicillium muscarium against Bemisia tabaci (Genn.) under greenhouse conditions in Tunisia

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Besma Hamrouni Assadi ◽  
Sabrine Chouikhi ◽  
Refki Ettaib ◽  
Naima Boughalleb M’hamdi ◽  
Mohamed Sadok Belkadhi
Keyword(s):  
Beauveria Bassiana ◽  
Bemisia Tabaci ◽  
Entomopathogenic Fungi ◽  
Tobacco Plant ◽  
Economic Losses ◽  
Native Strain ◽  
Lecanicillium Muscarium ◽  
Southern Tunisia ◽  
Significant Efficacy ◽  
Nesidiocoris Tenuis

Abstract Background The misuse of chemical insecticides has developed the phenomenon of habituation in the whitefly Bemisia tabaci (Gennadius) causing enormous economic losses under geothermal greenhouses in southern Tunisia. Results In order to develop means of biological control appropriate to the conditions of southern Tunisia, the efficacy of the native strain of the predator Nesidiocoris tenuis Reuter (Hemiptera: Miridae) and two entomopathogenic fungi (EPF) Beauveria bassiana and Lecanicillium muscarium was tested against Bemisia tabaci (Gennadius). Indeed, the introduction of N. tenuis in doses of 1, 2, 3, or 4 nymphs per tobacco plant infested by the whitefly led to highly significant reduction in the population of B. tabaci, than the control devoid of predator. The efficacy of N. tenuis was very high against nymphs and adults of B. tabaci at all doses per plant with a rate of 98%. Likewise, B. bassiana and L. muscarium, compared to an untreated control, showed a very significant efficacy against larvae and adults of B. tabaci. In addition, the number of live nymphs of N. tenuis treated directly or introduced on nymphs of B. tabaci treated with the EPF remained relatively high, exceeding 24.8 nymphs per cage compared to the control (28.6). Conclusions It can be concluded that the native strain of N. tenuis and the EPF tested separately were effective against B. tabaci. Their combined use appears to be possible.

scholarly journals On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Shehzad ◽  
Muhammad Tariq ◽  
Tariq Mukhtar ◽  
Asim Gulzar
Keyword(s):  
Sex Ratio ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Insect Pest ◽  
Diamondback Moth ◽  
Economic Damage ◽  
Offspring Sex Ratio ◽  
Development Of Resistance

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.

scholarly journals The Psychonauts’ Benzodiazepines; Quantitative Structure-Activity Relationship (QSAR) Analysis and Docking Prediction of Their Biological Activity

2021 ◽  
Vol 14 (8) ◽  
pp. 720
Author(s):  
Valeria Catalani ◽  
Michelle Botha ◽  
John Martin Corkery ◽  
Amira Guirguis ◽  
Alessandro Vento ◽  
...  
Keyword(s):  
Biological Activity ◽  
Computational Models ◽  
Quantitative Structure Activity Relationship ◽  
Structure Activity Relationship ◽  
Docking Studies ◽  
Health Concern ◽  
Activity Relationship ◽  
Quantitative Structure ◽  
High Biological Activity ◽  
Structure Activity

Designer benzodiazepines (DBZDs) represent a serious health concern and are increasingly reported in polydrug consumption-related fatalities. When new DBZDs are identified, very limited information is available on their pharmacodynamics. Here, computational models (i.e., quantitative structure-activity relationship/QSAR and Molecular Docking) were used to analyse DBZDs identified online by an automated web crawler (NPSfinder®) and to predict their possible activity/affinity on the gamma-aminobutyric acid A receptors (GABA-ARs). The computational software MOE was used to calculate 2D QSAR models, perform docking studies on crystallised GABA-A receptors (6HUO, 6HUP) and generate pharmacophore queries from the docking conformational results. 101 DBZDs were identified online by NPSfinder®. The validated QSAR model predicted high biological activity values for 41% of these DBDZs. These predictions were supported by the docking studies (good binding affinity) and the pharmacophore modelling confirmed the importance of the presence and location of hydrophobic and polar functions identified by QSAR. This study confirms once again the importance of web-based analysis in the assessment of drug scenarios (DBZDs), and how computational models could be used to acquire fast and reliable information on biological activity for index novel DBZDs, as preliminary data for further investigations.

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