scholarly journals Registration of entomopatogenic micromycete Cordyceps sp. in bloodsucking blackflies Wilhelmia equine (Linnaeus, 1758)

2020 ◽  
Vol 65 (1) ◽  
pp. 43-49
Author(s):  
D. V. Dovnar ◽  
E. N. Yankovskaya ◽  
D. V. Voitka ◽  
O. Yu. Baranov ◽  
V. M. Kaplich ◽  
...  
Keyword(s):  
Biological Activity ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Comparative Evaluation ◽  
Molecular Genetic ◽  
Biological Efficiency ◽  
In Vitro Experiments ◽  
Test Objects ◽  
Significant Mortality

An entomopathogenic fungi belonging to the genus Cordyceps was isolated from the affected larvae of the bloodsucking blackflies Wilhelmia equina (Linnaeus, 1758). Larval stage of Simulium were collected on the territory of the Lepel district of the Vitebsk region in the river Essa in July 2018. Isolate was identified using cultural-morphological and molecular-genetic methods.In vitro experiments comparative evaluation of biological activity of isolated strain Cordyceps sp. and Beauveria bassiana 10-06 was carried out for 3rd instar larvae of W. equina. Two different concentrations 1∙106 and 1∙107 conidia/ml were made and tested in 3 replications. On the 3rd day of incubation both fungi caused a significant mortality of test-objects (p = 0.03) compared to control. The biological efficiency of the concentration of 107 соnidia/ml of the strain Beauveria bassiana 10-06 on the 3rd day was 94.4 %, on the 4th – 98.3 %, of the strain Cordyceps sp. – 76.0 and 88.9 % respectively.

scholarly journals Effect of Biofertilizers and Neem Oil on the Entomopathogenic Fungi Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok

2001 ◽  
Vol 44 (4) ◽  
pp. 419-423 ◽  
Author(s):  
Edson Hirose ◽  
Pedro M. O. J. Neves ◽  
João A. C. Zequi ◽  
Luís H. Martins ◽  
Cristiane H. Peralta ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Azadirachta Indica ◽  
Organic Agriculture ◽  
Metarhizium Anisopliae ◽  
Neem Oil ◽  
Colony Diameter ◽  
Negative Effect ◽  
Fungitoxic Effect

The in vitro fungitoxic effect of three biofertilizers, E.M.-4, Multibion <FONT FACE=Symbol>Ô</FONT> and Supermagro used in organic agriculture and the neem oil (Azadirachta indica A. Juss) on the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana was studied. These products were mixed in a medium where the two fungi were inoculated, and germination, vegetative growth and conidiogenesis were assessed. The biofertilizers Supermagro and E.M.- 4 showed to be less toxic for the two fungi whereas Multibion<FONT FACE=Symbol>Ô</FONT> caused major inhibition on M. anisopliae, with reductions in germination (-37.74%), colony diameter (-30.26%) and conidiogenesis (-42.62%). Neem oil promoted a larger negative effect on B. bassiana, inhibiting germination (-45.27%), colony diameter (-36.62%) and conidiogenesis (-84.93%).

scholarly journals Effect of Agrochemicals Used in the Cultivation of Soybean and Irrigated Rice on Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok

2019 ◽  
Vol 11 (17) ◽  
pp. 167
Author(s):  
Vanessa P. Gonçalves ◽  
Cândida R. J. de Farias ◽  
Victoria Moreira-Nunêz ◽  
Renata Moccellin ◽  
Viviana Gaviria-Hernández ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Spore Germination ◽  
Metarhizium Anisopliae ◽  
Mycelial Growth ◽  
Potato Dextrose Agar ◽  
Irrigated Rice ◽  
Spray Method ◽  
Fungitoxic Effect

The objective of this work was to evaluate the fungitoxic effect of the agrochemicals used in the cultivation of soybean and irrigated rice on entomopathogenic fungi such as Beauveria bassiana and Metarhizium anisopliae by means of the mycelial growth, sporulation and spore germination. The isolates were inoculated in potato-dextrose-agar (PDA) medium containing the pesticides and exposed to spraying with the products. It was observed that chlorantraniliprole produced the best results regarding and was compatible with the two methods of contact of the product with the fungi. Flubendiamide it&rsquo;s very toxic to fungi, producing 100% inhibition when incorporated into the medium, when by spraying, the fungus M. anisopliae got mycelial growth. Etofenprox and thiamethoxam changed their toxity classification according to the method of contact with product, was moderately compatible to B. bassiana and compatible to M. anisopliae and spinosad showed more compatibility with M. anisopliae than B. bassiana and was classified as compatible. Among fungicides the tricyclazole was the only compatible with the fungus by the spray method. The results showed that in vitro agrochemicals such as tricyclazole, thiamethoxam, flubendiamide and etofenprox are harmful to fungi. It was found that environmental interference could minimize the effects on organisms, especially when the chemicals are applied by spraying.

SCREENING OF COLLECTION STRAINS (COLLECTIONS) OF ENTOMOPATHOGENIC FUNGI AGAINST ASIAN LOCUSTS FOR SIGNS OF VIRULENCE

Habarshy ◽  
2021 ◽  
pp. 124-135
Author(s):  
U.T. Zhumatayeva ◽  
B.A. Duisembekov ◽  
A.M. Begalieva ◽  
U.O. Sabdenova ◽  
K.K. Yerkekulova
Keyword(s):  
Biological Activity ◽  
Mortality Rate ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Host Organism ◽  
High Biological Activity ◽  
Preliminary Screening ◽  
Test Organisms ◽  
Control Variant

The article provides a preliminary screening of Asian locust larvae aged 2-3 years for the virulence of 25 isolates isolated from insects belonging to different systematic groups of fungi Beauveria bassiana. Five of the 25 strains showed high biological activity against larvae, (BCo1-14, BSc1-15, BSc2-15, BTr1-16, BPit-16) reaching a mortality rate of 90-100% 21 days after inoculation. The strains that have the best effect on the level and rate of mortality of the host organism are - BCo1-14, BSc1-15, BSc2-15, BTr1-16, BPit-16. The greatest biological activity was observed in the BSc1-15 strain, as the mortality rate of test organisms was only 100% within 15 days of exposure. The lowest activity was shown by the BCi4-14 strain in the range of 35.0-62.5%. At the same time, the mortality rate of Asian locust larvae in the control variant (without treatment) for 21 days was 15.0±2.88%. Thus, the proportion of highly toxic forms (80-100% lethality) in the total number of studied strains was not more than 44%, and the proportion of mildly toxic forms (less than 80% activity) was 56%.

scholarly journals Isolation of Beauveria Strains and Their Potential as Control Agents for Lema bilineata Germar (Coleoptera: Chrysomelidae)

Insects ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 93
Author(s):  
Jason Lee Furuie ◽  
Andressa Katiski da Costa Stuart ◽  
Morgana Ferreira Voidaleski ◽  
Maria Aparecida Cassilha Zawadneak ◽  
Ida Chapaval Pimentel
Keyword(s):  
Molecular Markers ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Biocontrol Agent ◽  
Physalis Peruviana ◽  
Solanaceae Species ◽  
Ovicidal Effect ◽  
Ovicidal Effects ◽  
Commercial Field

Lema bilineata Germar (Coleoptera: Chrysomelidae) was recently reported to damage Physalis peruviana crops in Brazil. Given the potential for inflicting damage on other Solanaceae species and the lack of alternatives for controlling this pest, we assessed the pathogenicity of 15 Beauveria isolates against L. bilineata adults in vitro. In addition, three of these isolates were tested for their ovicidal effect against L. bilineata eggs. Fungal strains were isolated from mummified corpses of L. bilineata collected in a non-commercial field in Paraná, Brazil. The isolates were identified as Beauveria bassiana using molecular markers. Lema bilineata adults were susceptible to conidial suspensions of all these isolates at a concentration of 108 conidia mL−1. Deaths caused by fungal extrusion were confirmed. Three strains were found to be more virulent against L. bilineata adults and showed ovicidal effects. This is the first study on entomopathogenic fungi isolated from dead insects collected from P. peruviana crops and tested against L. bilineata carried out in Brazil. The results obtained in the laboratory indicate the high potential of the use of three B. bassiana strains against L. bilineata as a biocontrol agent.

scholarly journals Development of Biopesticide Bauveria bassiana Bals. (Vuill.) Fungi For Pest Control Vegetable in Berastagi

2018 ◽  
Vol 1 (2) ◽  
pp. 72-77
Author(s):  
Sularno Sularno
Keyword(s):  
Beauveria Bassiana ◽  
Pest Control ◽  
Entomopathogenic Fungi ◽  
Research Method ◽  
Agricultural Land ◽  
Artificial Medium ◽  
The Third ◽  
Two Stages ◽  
In Vitro Treatment

This research was conducted in two stages. The research method is the isolation and application of biopesticide. In the first stage aims to isolate entomopathogenic fungi Beauveria bassiana around Berastagi vegetable farms and grow on agar and corn mashed artificial medium. Testing the virulence of Beauveria bassiana in vitro in several types of vegetable pests before it is applied on agricultural land.  In phase II aims to application B. bassiana in the vegetable patch. In the in vitro treatment, the ability to infect insects beauvria average reached 75%. , Controls on crop lands, in the third week began their Crocidolomia pests that attack the growing point so that the cabbage plants fail to bloom. Average cropland applied biopesticides B. bassiana has been no attack. At week 7 attacks on control plants reach 13 plants or 17.3%. In field applied while Beauveria bassiana attack reached six plants, or about 8%.

scholarly journals Synthesis and biological activity of raltitrexed-carrier conjugates.

2010 ◽  
Vol 57 (1) ◽  
Author(s):  
Monika Jagiello ◽  
Urszula Kanska ◽  
Dmitry Nevozhay ◽  
Janusz Boratynski
Keyword(s):  
Cell Cycle ◽  
Aqueous Solution ◽  
Biological Activity ◽  
Covalent Binding ◽  
Maximum Yield ◽  
Free Drug ◽  
S Phase ◽  
In Vitro Experiments ◽  
M Phase

Drugs used in chemotherapy give undesirable side effects, e.g., cardiotoxicity, leucopenia, hair loss and others. Covalent binding of a drug with a carrier may change its biodistribution, elimination and/or rate of transformation in the organism. The aim of this work was to synthesize conjugates of anticancer drug - raltitrexed (RTX) with lysozyme, bovine serum albumin (BSA), and dextran T40 and to investigate their cytotoxicity and influence on the cell cycle in comparison with the free drug. Before conjugation RTX was transformed into anhydride by treatment with dicyclohexylcarbodiimide in dimethylformamide. Activated RTX was added into aqueous solution of carriers at different pH (from 8.5 to 10.5) for 3 to 15 min. The reaction was stopped by reducing the pH to 7.0. Maximum yield of the reaction was obtained at pH 10 for BSA as well as for dextran. The highest level of substitution was obtained after 5 min of the reaction. In in vitro experiments on three cell lines: SW707, LoVo and A549, all conjugates tested had up to a few hundred times higher IC(50) than the free drug. Interestingly, it was noticed that the conjugates based on dextran and albumin were more cytotoxic than the free drug in the highest concentrations tested (1000 and 10000 ng/ml). The influence of RTX and the conjugates on SW707 cell cycle was studied. RTX blocked the cell cycle mostly in the G(0)-G(1) and S phase and increased the percentage of apoptotic cells. Cells in the G(2)-M phase were not observed. The conjugates blocked the cell cycle in the S phase and decreased the percentage of cells in the G(0)-G(1) phase.

scholarly journals KOMPATIBILITAS CENDAWAN ENTOMOPATOGEN BEAUVERIA BASSIANA (BALS) VUILL (DEUTEROMYCOTINA: HYPHOMYCETES) DENGAN MINYAK SERAI WANGI

2012 ◽  
Vol 12 (1) ◽  
pp. 78-84
Author(s):  
Trizelia Trizelia ◽  
Rusdi Rusli
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Entomopathogenic Fungus ◽  
Colony Growth ◽  
Conidial Germination ◽  
Natural Control ◽  
Conidia Germination ◽  
Potential Source ◽  
Lemongrass Oil

Entomopathogenic fungi  such as Beauveria bassiana are important natural control agents of many insects and can be potentially used as a bioinsecticide against several pests. Other potential source of bioinsecticide is certain plants such as fragrant lemongrass oil.  The in vitro compatibility of the entomopathogenic fungus B. bassiana  and  fragrant lemongrass oil was evaluated.  Fragrant lemongrass oil was tested in three different concentrations (0.1, 0.3 and 0.5%). Fragrant lemongrass  oil  was diluted in the steril SDAY medium at the different concentrations. Effects of these concentrations on conidia germination, colony growth and sporulation were compared.  Fragrant lemongrass  oil  affected conidial germination, colony growth and sporulation of B. bassiana. Fragrant lemongrass  oil  was not compatible with the entomopathogenic fungus B.  bassiana.

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