scholarly journals Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures

10.3791/1523 ◽  
2009 ◽  
Author(s):  
Katharina Schallmoser ◽  
Dirk Strunk
Keyword(s):  
Stem Cell ◽  
Cell Cultures ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Human Stem Cell ◽  
Serum Free ◽  
Human Platelet Lysate

scholarly journals Gelatin-Methacryloyl (GelMA) Formulated with Human Platelet Lysate Supports Mesenchymal Stem Cell Proliferation and Differentiation and Enhances the Hydrogel’s Mechanical Properties

2019 ◽  
Vol 6 (3) ◽  
pp. 76 ◽  
Author(s):  
Marline Kirsch ◽  
Luise Birnstein ◽  
Iliyana Pepelanova ◽  
Wiebke Handke ◽  
Jessica Rach ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Cell Cultures ◽  
Human Platelet ◽  
3D Cell Culture ◽  
Platelet Lysate ◽  
Dependent Manner ◽  
Human Platelet Lysate

Three-dimensional (3D) cell culture is a major focus of current research, since cultivation under physiological conditions provides more reliable information about in vivo cell behavior. 3D cell cultures are used in basic research to better understand intercellular and cell-matrix interactions. However, 3D cell culture plays an increasingly important role in the in vitro testing of bioactive substances and tissue engineering. Gelatin-methacryloyl (GelMA) hydrogels of different degrees of functionalization (DoFs) are a versatile tool for 3D cell culture and related applications such as bioprinting. Human platelet lysate (hPL) has already demonstrated positive effects on 2D cell cultures of different cell types and has proven a valuable alternative to fetal calf serum (FCS). Traditionally, all hydrogels are formulated using buffers. In this study, we supplemented GelMA hydrogels of different DoF with hPL during adipose tissue-derived mesenchymal stem cell (AD-MSCs) encapsulation. We studied the effect of hPL supplementation on the spreading, proliferation, and osteogenic differentiation of AD-MSCs. In addition, the influence of hPL on hydrogel properties was also investigated. We demonstrate that the addition of hPL enhanced AD-MSC spreading, proliferation, and osteogenic differentiation in a concentration-dependent manner. Moreover, the addition of hPL also increased GelMA viscosity and stiffness.

TRANSPLANTATION AND CELLULAR ENGINEERING: Adipose tissue mesenchymal stem cell expansion in animal serum-free medium supplemented with autologous human platelet lysate

Transfusion ◽  
2009 ◽  
Vol 49 (12) ◽  
pp. 2680-2685 ◽  
Author(s):  
Ivan Souza Blande ◽  
Vinicius Bassaneze ◽  
Carolina Lavini-Ramos ◽  
Kellen Cristhina Fae ◽  
Jorge Kalil ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stem Cell ◽  
Cell Expansion ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Stem Cell Expansion ◽  
Human Platelet Lysate

EFFECT OF HUMAN PLATELET LYSATE ON HUMAN MESENCHYMAIS STEM CELL SUPPLEMENTATION AND APPLICATION IN ACUTE NEURAL INJURY MODEL: STAB WOUND

Cytotherapy ◽  
2021 ◽  
Vol 23 (4) ◽  
pp. 11-12
Author(s):  
K.R.D Silva ◽  
DC de Almeida ◽  
SA Romariz ◽  
BM Longo ◽  
NOS Câmara
Keyword(s):  
Stem Cell ◽  
Stab Wound ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Neural Injury ◽  
Injury Model ◽  
Human Platelet Lysate

Effect of human platelet lysate supplemented medium on human mesenchymal stem cell identity and immunomodulatory capacity

Cytotherapy ◽  
2017 ◽  
Vol 19 (5) ◽  
pp. S151
Author(s):  
S. Cañadillas ◽  
M.D. Carmona ◽  
M.S. Gonzalez ◽  
S. Nogueras ◽  
V. Martín-Palanco ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Human Platelet ◽  
Human Mesenchymal Stem Cell ◽  
Platelet Lysate ◽  
Cell Identity ◽  
Immunomodulatory Capacity ◽  
Human Platelet Lysate
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