scholarly journals Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation

10.3791/51552 ◽  
2014 ◽  
Author(s):  
Kathirvel Kandasamy ◽  
Kaushik Parthasarathi
Keyword(s):  
Rat Lung ◽  
Microvessel Permeability ◽  
Lung Preparation

scholarly journals Analysis of ET-A and ET-B receptors using an isolated perfused rat lung preparation

2004 ◽  
Vol 181 (2) ◽  
pp. 259-264 ◽  
Author(s):  
B. Granstrom ◽  
E. Nilsson ◽  
U. Hultkvist-Bengtsson ◽  
L. Edvinsson
Keyword(s):  
Rat Lung ◽  
Lung Preparation

Isolated perfused rat lung preparation.

1966 ◽  
Vol 21 (1) ◽  
pp. 313-316 ◽  
Author(s):  
S Levey ◽  
R Gast
Keyword(s):  
Rat Lung ◽  
Lung Preparation

Stability of a kininase of microbial origin in the blood perfusates of an isolated rat lung preparation

Life Sciences ◽  
1968 ◽  
Vol 7 (18) ◽  
pp. 921-927 ◽  
Author(s):  
K. Haavik Nilsen ◽  
H.E. Rugstad
Keyword(s):  
Rat Lung ◽  
Microbial Origin ◽  
Isolated Rat Lung ◽  
Lung Preparation ◽  
Isolated Rat

Effect of tumor necrosis factor on hypoxic pulmonary vasoconstriction

1992 ◽  
Vol 72 (3) ◽  
pp. 1044-1049 ◽  
Author(s):  
S. F. Liu ◽  
A. Dewar ◽  
D. E. Crawley ◽  
P. J. Barnes ◽  
T. W. Evans
Keyword(s):  
Endothelial Dysfunction ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Hypoxic Pulmonary Vasoconstriction ◽  
Detectable Effect ◽  
Rat Lung ◽  
Pulmonary Vasoconstriction ◽  
Endothelium Dependent Relaxation ◽  
Necrosis Factor ◽  
Lung Preparation

The effects of tumor necrosis factor (TNF) on hypoxic pulmonary vasoconstriction (HPV) and endothelium-dependent relaxation were examined in a blood-perfused rat lung preparation. Lungs from TNF-treated rats (0.26 mg/kg iv 12 h before experimentation) had a significantly greater HPV and a reduced vasorelaxant response to the endothelium-dependent vasodilator acetylcholine (ACh) but a similar vasorelaxant response to the endothelium-independent vasodilator nitroprusside compared with lungs from control rats (pretreated with 0.1 ml saline iv). Pentoxifylline (20 mg/kg iv and ip 20 min before administration of TNF) had no detectable effect on either HPV or ACh-induced relaxation but completely negated the augmentation on HPV and the inhibiting action on ACh-induced relaxation caused by TNF. The TNF effect on ACh relaxation was unaffected by pretreatment with L-arginine. These results indicate that TNF induces endothelial dysfunction and enhances HPV, effects that are inhibited by pentoxifylline.

Regulation of lung surfactant secretion by intracellular pH

1989 ◽  
Vol 257 (6) ◽  
pp. L354-L360 ◽  
Author(s):  
A. Chander
Keyword(s):  
Lung Surfactant ◽  
Lavage Fluid ◽  
Rat Lung ◽  
Perfusion Medium ◽  
Bicarbonate Buffer ◽  
Low Co2 ◽  
Surfactant Secretion ◽  
Lung Lipid ◽  
Lung Preparation

We investigated secretion of lung surfactant phosphatidylcholine (PC) using isolated perfused rat lung preparation after labeling the lung lipids in vitro with [methyl-3H]choline. The perfusion medium was Krebs-Ringer bicarbonate buffer (pH 7.4) containing 10 mM glucose and 3% fatty acid-poor bovine serum albumin. After ventilation of lungs with air containing 5% CO2 (control) for 1 h, 0.91% +/- 0.04 (mean +/- SE, n = 6) of total lung lipid radioactivity (greater than 95% in PC) was recovered in the cell-free lavage fluid. The secretion of PC was increased with terbutaline (50 microM), 8-bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP, 100 microM), phorbol L2-myristate 13-acetate (30 ng/ml), and ATP (1 mM), in each case by approximately 150%. Secretion of PC was also increased by 160% if the lungs were ventilated with air containing 0% CO2. The low CO2-mediated PC secretion was time and concentration dependent. The dose-response curve for 0-10% CO2 was S-shaped. The low CO2-induced increase in PC secretion could be largely reversed with diffusible weak acids (25 mM, acetate or butyrate) in the perfusion medium. An increase (70%) in secretion was also induced with 10 mM NH4Cl, suggesting a role for intracellular alkalosis. These observations suggest that intracellular alkalosis stimulates lung surfactant secretion. Alkalosis-stimulated secretion of PC was additive with that with terbutaline (5 X 10(-7) to 5 X 10(-4) M) or 10(-4) M 8-BrcAMP, suggesting that alkalosis effect was not mediated through the beta-adrenergic pathway of surfactant secretion.(ABSTRACT TRUNCATED AT 250 WORDS)

Sensitivity of bronchopulmonary receptors to cold and heat mediated by transient receptor potential cation channel subtypes in an ex vivo rat lung preparation

2011 ◽  
Vol 177 (3) ◽  
pp. 327-332 ◽  
Author(s):  
Yun Zhou ◽  
Biying Sun ◽  
Qian Li ◽  
Pin Luo ◽  
Li Dong ◽  
...  
Keyword(s):  
Transient Receptor Potential ◽  
Ex Vivo ◽  
Receptor Potential ◽  
Cation Channel ◽  
Rat Lung ◽  
Transient Receptor ◽  
Lung Preparation

scholarly journals An isolated perfused rat lung preparation.

1976 ◽  
Vol 16 ◽  
pp. 61-66 ◽  
Author(s):  
S L Young
Keyword(s):  
Rat Lung ◽  
Lung Preparation

An Isolated Perfused Rat Lung Preparation for the Study of Aerosolized Drug Deposition and Absorption

1986 ◽  
Vol 75 (2) ◽  
pp. 168-171 ◽  
Author(s):  
Peter R. Byron ◽  
Sian R.N. Roberts ◽  
A. Ruth Clark
Keyword(s):  
Rat Lung ◽  
Drug Deposition ◽  
Lung Preparation

Replication of Internal Biological Surfaces

1972 ◽  
Vol 30 ◽  
pp. 308-309
Author(s):  
J. A. Nowell ◽  
J. Pangborn ◽  
W. S. Tyler
Keyword(s):  
Da Vinci ◽  
Leonardo Da Vinci ◽  
Rat Lung ◽  
Tubular Structures ◽  
Cerebral Ventricles ◽  
Internal Surfaces ◽  
Biological Surfaces ◽  
Dog Kidney ◽  
Lung And Kidney ◽  
Scanning Electron

Leonardo da Vinci in the 16th century, used injection replica techniques to study internal surfaces of the cerebral ventricles. Developments in replicating media have made it possible for modern morphologists to examine injection replicas of lung and kidney with the scanning electron microscope (SEM). Deeply concave surfaces and interrelationships to tubular structures are difficult to examine with the SEM. Injection replicas convert concavities to convexities and tubes to rods, overcoming these difficulties.Batson's plastic was injected into the renal artery of a horse kidney. Latex was injected into the pulmonary artery and cementex in the trachea of a cat. Following polymerization the tissues were removed by digestion in concentrated HCl. Slices of dog kidney were aldehyde fixed by immersion. Rat lung was aldehyde fixed by perfusion via the trachea at 30 cm H2O. Pieces of tissue 10 x 10 x 2 mm were critical point dried using CO2. Selected areas of replicas and tissues were coated with silver and gold and examined with the SEM.

Sign in / Sign up

Export Citation Format

Share Document