scholarly journals Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

10.3791/57164 ◽  
2018 ◽  
Author(s):  
Adam C. Parslow ◽  
Andrew H.A. Clayton ◽  
Peter Lock ◽  
Andrew M. Scott
Keyword(s):  
Confocal Microscopy ◽  
Cell Surface ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Cell Surface Receptor ◽  
Receptor Aggregation ◽  
Surface Receptor ◽  
Image Correlation Spectroscopy

Sendai Virus Binds to a Dispersed Population of NBD-GD1a

2000 ◽  
Vol 20 (3) ◽  
pp. 139-155 ◽  
Author(s):  
Jonathan V. Rocheleau ◽  
Nils O. Petersen
Keyword(s):  
Target Cell ◽  
Sendai Virus ◽  
Correlation Spectroscopy ◽  
Image Correlation ◽  
Enveloped Virus ◽  
Receptor Aggregation ◽  
Image Correlation Spectroscopy ◽  
Ganglioside Gd1a ◽  
Dose Dependent ◽  
Two Populations

Receptor aggregation is believed to be an important step in the attachmentof membrane enveloped virus' to target cell membranes. A likely receptorfor Sendai virus is the ganglioside GD1a. In this work we have studied themembrane diffusion of the fluorescent ganglioside NBD-GD1a on the surfaceof CV-1 cells with standard photobleaching techniques. Using confocallaser scanning microscopy (CLSM) and Image Correlation Spectroscopy(ICS) NBD-GD1a is shown to exist in at least two populations: dispersedand aggregated. By quantifying the distribution of NBD-GD1a pre- andpost-incubation with Sendai virus it is shown that the virus inducesa dose-dependent clustering of NBD-GD1a. Image cross-correlationspectroscopy (ICCS) is used to further quantitatively characterizethis clustering by demonstrating that it occurs due to binding ofvirus to the dispersed population of NBD-GD1a.

A model of cell surface receptor aggregation

2017 ◽  
Vol 75 (3) ◽  
pp. 705-731 ◽  
Author(s):  
D. Iron ◽  
J. Rumsey
Keyword(s):  
Cell Surface ◽  
Cell Surface Receptor ◽  
Receptor Aggregation ◽  
Surface Receptor

Expression, function, and localization of the REG cell surface receptor

2001 ◽  
Vol 120 (5) ◽  
pp. A18-A19
Author(s):  
B DIECKGRAEFE ◽  
C HOUCHEN ◽  
H ZHANG
Keyword(s):  
Cell Surface ◽  
Cell Surface Receptor ◽  
Surface Receptor
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