scholarly journals Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells

10.3791/59697 ◽  
2019 ◽  
Author(s):  
Tenaya K. Vallery ◽  
Joan A. Steitz
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Specific Gene ◽  
Gene Products ◽  
Infected Cells ◽  
Quantitative Fluorescence

Interphase Quantitative Fluorescence in Situ Hybridization (IQ-FISH)

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Ivan Y. Iourov ◽  
◽  
Ilia V. Soloviev ◽  
Yuri B. Yurov ◽  
Svetlana G. Vorsanova ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Quantitative Fluorescence

scholarly journals Quantitative Fluorescence in Situ Hybridization in Lung Cancer as a Diagnostic Marker

1999 ◽  
Vol 1 (1) ◽  
pp. 33-37 ◽  
Author(s):  
Khuong Truong ◽  
Michèle Gerbault-Seureau ◽  
Marie-Noëlle Guilly ◽  
Philippe Vielh ◽  
Gérard Zalcman ◽  
...  
Keyword(s):  
Lung Cancer ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Diagnostic Marker ◽  
Quantitative Fluorescence

scholarly journals Telomere Length of Human Adult Bronchial Epithelium and Bronchogenic Squamous Cell Carcinoma Measured Using Tissue Quantitative Fluorescence in situ Hybridization

Respiration ◽  
2015 ◽  
Vol 90 (4) ◽  
pp. 321-326 ◽  
Author(s):  
Shinsuke Aida ◽  
Junko Aida ◽  
Kayo Hasegawa ◽  
Toshio Kumasaka ◽  
Hideyuki Shimazaki ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cell Carcinoma ◽  
Telomere Length ◽  
Squamous Cell ◽  
Bronchial Epithelium ◽  
Human Adult ◽  
Quantitative Fluorescence

Telomere length variations in 6 mucosal cell types of gastric tissue observed using a novel quantitative fluorescence in situ hybridization method

2007 ◽  
Vol 38 (8) ◽  
pp. 1192-1200 ◽  
Author(s):  
Junko Aida ◽  
Naotaka Izumiyama-Shimomura ◽  
Ken-ichi Nakamura ◽  
Akio Ishii ◽  
Naoshi Ishikawa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Telomere Length ◽  
Cell Types ◽  
Mucosal Cell ◽  
Gastric Tissue ◽  
Hybridization Method ◽  
Quantitative Fluorescence

Arm-specific telomere dynamics of each individual chromosome in induced pluripotent stem cells revealed by quantitative fluorescence in situ hybridization

2014 ◽  
Vol 46 (6) ◽  
pp. 470-476 ◽  
Author(s):  
Masanori Terai ◽  
Naotaka Izumiyama-Shimomura ◽  
Junko Aida ◽  
Naoshi Ishikawa ◽  
Mie Kuroiwa ◽  
...  
Keyword(s):  
Stem Cells ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Individual Chromosome ◽  
Induced Pluripotent ◽  
Quantitative Fluorescence ◽  
Telomere Dynamics

scholarly journals Detection of Ampicillin-Resistant E. coli Using Novel Nanoprobe-Combined Fluorescence In Situ Hybridization

Nanomaterials ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 750 ◽  
Author(s):  
Wang Sik Lee ◽  
Soohyun Lee ◽  
Taejoon Kang ◽  
Choong-Min Ryu ◽  
Jinyoung Jeong
Keyword(s):  
Antibiotic Resistance ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Antibiotic Resistance Gene ◽  
Sol Gel ◽  
Light Condition ◽  
Resistant Bacteria ◽  
Specific Gene ◽  
E Coli

Antibiotic-resistant bacteria present a global threat because the infections they cause are difficult to treat. Therefore, it is highly important to develop advanced methods for the identification of antibiotic resistance gene in the virulent bacteria. Here, we report the development of novel nanoprobes for fluorescence in situ hybridization (FISH) and the application of the nanoprobe to the detection of ampicillin-resistant Escherichia coli. The nanoprobe for FISH was synthesized by the modified sol–gel chemistry and the synthesized nanoprobe provided strong fluorescent signals and pH stability even under natural light condition. For the double-identification of bacteria species and ampicillin-resistance with a single probe in situ, the nanoprobes were conjugated to the two kinds of biotinylated probe DNAs; one for E. coli-species specific gene and the other for a drug-resistant gene. By using the nanoprobe-DNA conjugants, we successfully detected the ampicillin-resistant E. coli through the FISH technique. This result suggests the new insight into light stable FISH application of the nanoprobe for a pathogenic antibiotic-resistance bacterium.

Telomere Metabolism and Diagnostic Demonstration of Telomere Measurement in the Human Esophagus for Distinguishing Benign from Malignant Tissue by Tissue Quantitative Fluorescence in situ Hybridization

Oncology ◽  
2006 ◽  
Vol 71 (5-6) ◽  
pp. 430-436 ◽  
Author(s):  
Makoto Kammori ◽  
Naotaka Izumiyama ◽  
Ken-ichi Nakamura ◽  
Rie Kurabayashi ◽  
Mitsuhiko Kashio ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Malignant Tissue ◽  
Human Esophagus ◽  
Quantitative Fluorescence

Quantitative Fluorescence In Situ Hybridization Detection of Plant mRNAs with Single-Molecule Resolution

2020 ◽  
pp. 23-33
Author(s):  
Kun Huang ◽  
Mona Batish ◽  
Chong Teng ◽  
Alex Harkess ◽  
Blake C. Meyers ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Single Molecule ◽  
Quantitative Fluorescence ◽  
Hybridization Detection

Investigation of telomere length dynamics in induced pluripotent stem cells using quantitative fluorescence in situ hybridization

2013 ◽  
Vol 45 (6) ◽  
pp. 407-413 ◽  
Author(s):  
Masanori Terai ◽  
Naotaka Izumiyama-Shimomura ◽  
Junko Aida ◽  
Naoshi Ishikawa ◽  
Mie Kuroiwa ◽  
...  
Keyword(s):  
Stem Cells ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Induced Pluripotent Stem Cells ◽  
Telomere Length ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent ◽  
Quantitative Fluorescence
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