A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells

10.3791/59881 ◽  
2019 ◽  
Author(s):  
Jeong Hoon Lee ◽  
Jeong Mi Ha ◽  
Quynh Mai Ho ◽  
Chae Hun Leem
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Correction Method ◽  
Nicotinamide Adenine ◽  
Live Cells ◽  
Fura 2 ◽  
Intracellular Ca

scholarly journals Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Olivier Sallin ◽  
Luc Reymond ◽  
Corentin Gondrand ◽  
Fabio Raith ◽  
Birgit Koch ◽  
...  
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Dynamic Range ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Nicotinamide Adenine ◽  
Spatial Proximity ◽  
Live Cells ◽  
Fluorescent Biosensors ◽  
New Class ◽  
Metabolic Fluxes ◽  
Response Range

We introduce a new class of semisynthetic fluorescent biosensors for the quantification of free nicotinamide adenine dinucleotide (NAD+) and ratios of reduced to oxidized nicotinamide adenine dinucleotide phosphate (NADPH/NADP+) in live cells. Sensing is based on controlling the spatial proximity of two synthetic fluorophores by binding of NAD(P) to the protein component of the sensor. The sensors possess a large dynamic range, can be excited at long wavelengths, are pH-insensitive, have tunable response range and can be localized in different organelles. Ratios of free NADPH/NADP+ are found to be higher in mitochondria compared to those found in the nucleus and the cytosol. By recording free NADPH/NADP+ ratios in response to changes in environmental conditions, we observe how cells can react to such changes by adapting metabolic fluxes. Finally, we demonstrate how a comparison of the effect of drugs on cellular NAD(P) levels can be used to probe mechanisms of action.

Differentiation of apoptosis from necrosis by dynamic changes of reduced nicotinamide adenine dinucleotide fluorescence lifetime in live cells

2008 ◽  
Vol 13 (5) ◽  
pp. 054011 ◽  
Author(s):  
Hsing-Wen Wang ◽  
Vladimir Gukassyan ◽  
Chien-Tsun Chen ◽  
Yau-Huei Wei ◽  
Han-Wen Guo ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Nicotinamide Adenine Dinucleotide ◽  
Nicotinamide Adenine ◽  
Live Cells ◽  
Dynamic Changes ◽  
Reduced Nicotinamide Adenine Dinucleotide

Effect of Niacin on Mitochondria of Allium Cepa Root Cells

1967 ◽  
Vol 25 ◽  
pp. 78-79
Author(s):  
M. Arif Hayat
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Hydrogen Transfer ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Nicotinamide Adenine ◽  
Root Tips ◽  
Root Cells ◽  
Transfer Processes ◽  
Standard Procedures ◽  
A Cell ◽  
Critical Interest

Although it is recognized that niacin (pyridine-3-carboxylic acid), incorporated as the amide in nicotinamide adenine dinucleotide (NAD) or in nicotinamide adenine dinucleotide phosphate (NADP), is a cofactor in hydrogen transfer in numerous enzyme reactions in all organisms studied, virtually no information is available on the effect of this vitamin on a cell at the submicroscopic level. Since mitochondria act as sites for many hydrogen transfer processes, the possible response of mitochondria to niacin treatment is, therefore, of critical interest.Onion bulbs were placed on vials filled with double distilled water in the dark at 25°C. After two days the bulbs and newly developed root system were transferred to vials containing 0.1% niacin. Root tips were collected at ¼, ½, 1, 2, 4, and 8 hr. intervals after treatment. The tissues were fixed in glutaraldehyde-OsO4 as well as in 2% KMnO4 according to standard procedures. In both cases, the tissues were dehydrated in an acetone series and embedded in Reynolds' lead citrate for 3-10 minutes.

Nicotinamide Adenine Dinucleotide and the Metabolism of Ethanol and Acetaldehyde

1967 ◽  
Vol 28 (2) ◽  
pp. 213-224 ◽  
Author(s):  
E. Majchrowicz ◽  
B. L. Bercaw ◽  
W. M. Cole ◽  
D. H. Gregory
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Nicotinamide Adenine

Treatment of Acute Brain Syndrome from Alcohol with Nicotinamide Adenine Dinucleotide and Methaminodiazepoxide

1966 ◽  
Vol 27 (2) ◽  
pp. 214-220 ◽  
Author(s):  
Leon J. Hekimian ◽  
Arnold J. Friedhoff ◽  
Murray Alpert
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Nicotinamide Adenine
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