Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

10.3791/62728 ◽  
2021 ◽  
Author(s):  
Jared L. Dopp ◽  
Nigel F. Reuel
Keyword(s):  
Enzymatic Methods

CXCR4 expression by mesenchymal stromal cells is lost after use of enzymatic dissociation agents, but preserved by use of non-enzymatic methods

2021 ◽  
Author(s):  
Burcu Pervin ◽  
Gözde Aydın ◽  
Trudi Visser ◽  
Duygu Uçkan-Çetinkaya ◽  
Fatima S. F. Aerts-Kaya
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Cxcr4 Expression ◽  
Enzymatic Dissociation ◽  
Enzymatic Methods

Modification of Fats and Oils via Chemical and Enzymatic Methods

2020 ◽  
pp. 1-29
Author(s):  
S.P.J. Namal Senanayake ◽  
Fereidoon Shahidi
Keyword(s):  
Fats And Oils ◽  
Enzymatic Methods

Determination of process-related impurities in N-acetylglucosamine prepared by chemical and enzymatic methods: structural elucidation and quantification

2016 ◽  
Vol 39 (7) ◽  
pp. 937-945
Author(s):  
Yi Soo Kim ◽  
Sung Joong Lee ◽  
Jin Young Choi ◽  
Yun-Hi Kim ◽  
Kebede Taye Desta ◽  
...  
Keyword(s):  
Structural Elucidation ◽  
Related Impurities ◽  
Enzymatic Methods

Synthesis of cholecystokinin peptide CCK-4 exclusively by enzymatic methods

2002 ◽  
Vol 22 (4) ◽  
pp. 285-287
Author(s):  
Zimin Lü ◽  
Li Guo ◽  
Dietmar Huettner ◽  
Heiner Eckstein
Keyword(s):  
Enzymatic Methods

The prediction of digestibility by rumen fluid and enzymatic methods

1996 ◽  
Vol 1996 ◽  
pp. 211-211
Author(s):  
Peter Young ◽  
F. P. O'Mara ◽  
M. Rath ◽  
P. J. Caffrey
Keyword(s):  
Organic Matter ◽  
Rumen Fluid ◽  
Organic Matter Digestibility ◽  
Enzymatic Methods

Rumen fluid and cellulase based techniques are widely used to predict the digestibility of compound feeds and their ingredients. Recently gammanase enzymes have been added to some cellulase based techniques (Dowman, 1993; De Boever et al., 1994). Few comparisons of these techniques have involved by-product concentrate ingredients. The objective of this experiment was to compare the ability of three techniques, in vitro rumen fluid (RF), pepsin cellulase gammanase (PCG), and neutral detergent cellulase gammanase (NCDG), to predict the in vivo organic matter digestibility (OMD) of concentrate ingredients.

scholarly journals Human lipoproteins comprise at least 12 different classes that are 1 lognormally distributed.

2021 ◽  
Author(s):  
Tomokazu Konishi ◽  
Risako Fujiwara ◽  
Tadaaki Saito ◽  
Tadaaki Saito ◽  
Nozomi Satou ◽  
...  
Keyword(s):  
Human Serum ◽  
Separation Method ◽  
High Gravity ◽  
Serum Samples ◽  
Rapid Separation ◽  
Human Serum Samples ◽  
Multiple Factors ◽  
Mixed Classes ◽  
Time Required ◽  
Enzymatic Methods

Lipoproteins in medical samples have been measured by enzymatic methods that coincide with conventional ultracentrifugation. However, the high gravity and time required for ultracentrifugation can cause sample degradation. This study presents the results of HPLC, a gentler and rapid separation method, for 55 human serum samples. The elution patterns were analysed parametrically, and the attribute of each class was confirmed biochemically. Human samples contained 12 classes of lipoproteins, each of which may consist primarily of proteins. There are three classes of VLDLs. The level of each class was distributed lognormally, and the standard amount and the 95% range were estimated. Enzymatic methods measure the levels of several mixed classes. This lognormal character suggests that the levels are controlled by the synergy of multiple factors.

scholarly journals Enzymatic methods to predict the value of the energy and protein in feedingstuffs

1980 ◽  
Vol 29 (Hors-série) ◽  
pp. 325-340 ◽  
Author(s):  
D. F. OSBOURN ◽  
R. C. SIDDONS
Keyword(s):  
Enzymatic Methods
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