scholarly journals Bioconversion of Saw Dust Powder Acid Hydrolysis to Single Cell Protein by the Yeast Candida tropicalis

2021 ◽  
Vol 2 (02) ◽  
pp. 31-35
Author(s):  
Mustafa Haider
Keyword(s):  
Carbon Source ◽  
Single Cell ◽  
Candida Tropicalis ◽  
Basal Medium ◽  
Ammonium Phosphate ◽  
Dry Weight ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Low Grade ◽  
Saw Dust

This study was aimed to grow the local isolates yeast Candida tropicalis on locally prepared sawdust powder hydrolysate as a basal medium and carbon source with respect to single cell protein (SCP) production. The saw dust powder was treated with 10% H2SO4 for one hour at 100 0C. After cooling the supernatant containing the isolated sugar separated from debris by filtration and used as a carbon source and basal medium for yeast growth and SCP production. A high amount of SCP was achieved after five days of incubation, the percentage of the produced SCP 39.05% of the biomass dry weight, which is equivalent to 3.07g/l. SCP formation biosynthesis was affected by the level of nitrogen present in the medium; a high amount of protein being achieved in fermentation medium containing 0.3% urea in which the percentage of the yielded SCP was increased to 42.54%, (3.71g/l) of the biomass dry weight. The effect of varying nitrogen sources on SCP accumulation was also assayed. Medium containing ammonium phosphate greatly stimulated protein production in which the produced SCP was increased to reach 5.90 g/l. The produced amount equivalent to (48.22%) of the biomass dry weight. Conversely, a medium containing sodium nitrate had a suppressive effect on SCP production. This evidence clearly suggests that the metabolic versatility of Candida tropicalis may be employed in the conversion of low-grade material into high SCP product.

scholarly journals Candida tropicalis able to produce yeast single cell protein using sugarcane bagasse hemicellulosic hydrolysate as carbon source

2018 ◽  
Vol 2 (1) ◽  
pp. 19-21
Author(s):  
Cristina Emanuela Barbosa Magalhães ◽  
Manoel Santiago Souza-Neto ◽  
Spartaco Astolfi-Filho ◽  
Italo Thiago Silveira Rocha Matos
Keyword(s):  
Carbon Source ◽  
Single Cell ◽  
Sugarcane Bagasse ◽  
Candida Tropicalis ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Hemicellulosic Hydrolysate

Power-to-protein: converting renewable electric power and carbon dioxide into single cell protein with a two-stage bioprocess

2019 ◽  
Vol 12 (12) ◽  
pp. 3515-3521 ◽  
Author(s):  
Bastian Molitor ◽  
Akanksha Mishra ◽  
Largus T. Angenent
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Single Cell ◽  
Nitrogen Source ◽  
Electric Power ◽  
Circular Economy ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Waste Streams ◽  
Edible Protein

To prevent an environmental collapse while feeding a population of 10 billion people, dilute nitrogen in waste streams as a nitrogen source and carbon dioxide as a carbon source should be converted into edible protein as part of the circular economy.

scholarly journals Waste Cooking Oil as Substrate for Single Cell Protein Production by Yeast Yarrowia lipolytica

2020 ◽  
Vol 24 (3) ◽  
pp. 457-469
Author(s):  
Kriss Spalvins ◽  
Zane Geiba ◽  
Zane Kusnere ◽  
Dagnija Blumberga
Keyword(s):  
Carbon Source ◽  
Single Cell ◽  
Yarrowia Lipolytica ◽  
Protein Production ◽  
Waste Cooking Oil ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Cooking Oil ◽  
Cooking Oils ◽  
Single Cell Protein Production

AbstractCooking oils are widely used in food preparation. During cooking, harmful compounds are formed in oils, therefore utilization of used cooking oils (waste cooking oils) is limited. Single cell protein (SCP) is dietary protein, which can be produced from various protein-rich microorganisms that are capable of utilizing industrial by-products such as waste cooking oil (WCO). In this study the utilization of industrial WCO (obtained from local potato chips manufacturer) as a carbon source for single cell protein production by yeast Yarrowia lipolytica was assessed. The medium containing 27.5 g/L WCO and C/N ratio of 5–10 for batch fermentations was determined to be the optimal composition for SCP production. In this study, the highest reported Yarrowia lipolytica biomass concentration (57.37 g/L) was achieved when WCO was used as the main carbon source. Protein concentrations were relatively low (12.6 %), which also affected the final protein yield (7.23 g/L). The resulting biomass accumulated low concentrations of toxic malondialdehyde (MDA) (2.32 mg MDA/kg) compared to concentrations initially detected in the WCO itself (30.87 mg MDA/kg). To the best of the authors knowledge this is the first study to report on MDA decrease via microbial fermentations.

scholarly journals Addition of Carbon Sources to Pineapple Waste Media in the Production of Single Cell Protein Biomass Saccharomyces cerevisiae

2020 ◽  
Vol 2 (2) ◽  
pp. 70
Author(s):  
Anggi Nurmalasari ◽  
Shinta Maharani
Keyword(s):  
Single Cell ◽  
Protein Content ◽  
Carbon Sources ◽  
Dry Weight ◽  
Fermentation Medium ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Fermentation Time ◽  
Pineapple Peel ◽  
The Media

Single-cell protein (SCP) is the term used for crude or pure protein derived from simple single or multi-celled microorganisms. Pineapple peel contains monosaccharides as much as 10.8% so that it can be used as a fermentation medium in single-cell protein production. The purpose of this study was to determine the effect of adding carbon sources of fructose and sucrose on pH, cell dry weight, and protein content in the manufacture of single-cell proteins. This study used a completely randomized design (CRD) with two factors, namely the addition of carbon (fructose, sucrose, and control) and fermentation time (24.48, and 72 hours). The data analysis used the variance test and the Duncan Multiple Range Test (DMRT) continued to test with a confidence level of 95%. The results showed that the addition of carbon to the media had a very significant effect on media pH, cell dry weight, and protein content. In the medium with the addition of fructose it has a pH of 3.81; dry weight 0.4203 grams; and protein content 69.08/L. Whereas in the media with the addition of sucrose, the pH was 4.33, the dry weight of the cells was 0.3385 grams, and the cells had a protein content of 85.55 mg/L. The addition of a fructose carbon source gave the cell dry weight more than the addition of carbon sucrose

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