Power-to-protein: converting renewable electric power and carbon dioxide into single cell protein with a two-stage bioprocess

2019 ◽  
Vol 12 (12) ◽  
pp. 3515-3521 ◽  
Author(s):  
Bastian Molitor ◽  
Akanksha Mishra ◽  
Largus T. Angenent

To prevent an environmental collapse while feeding a population of 10 billion people, dilute nitrogen in waste streams as a nitrogen source and carbon dioxide as a carbon source should be converted into edible protein as part of the circular economy.

2018 ◽  
Vol 2 (1) ◽  
pp. 19-21
Author(s):  
Cristina Emanuela Barbosa Magalhães ◽  
Manoel Santiago Souza-Neto ◽  
Spartaco Astolfi-Filho ◽  
Italo Thiago Silveira Rocha Matos

2021 ◽  
Vol 2 (02) ◽  
pp. 31-35
Author(s):  
Mustafa Haider

This study was aimed to grow the local isolates yeast Candida tropicalis on locally prepared sawdust powder hydrolysate as a basal medium and carbon source with respect to single cell protein (SCP) production. The saw dust powder was treated with 10% H2SO4 for one hour at 100 0C. After cooling the supernatant containing the isolated sugar separated from debris by filtration and used as a carbon source and basal medium for yeast growth and SCP production. A high amount of SCP was achieved after five days of incubation, the percentage of the produced SCP 39.05% of the biomass dry weight, which is equivalent to 3.07g/l. SCP formation biosynthesis was affected by the level of nitrogen present in the medium; a high amount of protein being achieved in fermentation medium containing 0.3% urea in which the percentage of the yielded SCP was increased to 42.54%, (3.71g/l) of the biomass dry weight. The effect of varying nitrogen sources on SCP accumulation was also assayed. Medium containing ammonium phosphate greatly stimulated protein production in which the produced SCP was increased to reach 5.90 g/l. The produced amount equivalent to (48.22%) of the biomass dry weight. Conversely, a medium containing sodium nitrate had a suppressive effect on SCP production. This evidence clearly suggests that the metabolic versatility of Candida tropicalis may be employed in the conversion of low-grade material into high SCP product.


1996 ◽  
Vol 33 (8) ◽  
pp. 31-38 ◽  
Author(s):  
T. H. van der Westhuizen ◽  
W. A. Pretorius

A process was developed at the University of Pretoria through which it became possible to simultaneously purify organic effluents and produce a constant quality Single Cell Protein (SCP) product under non-aseptic conditions. Performance of the process as applied to a sugar-furfural effluent is described. The organism selected was Aspergillus fumigatus, a thermotolerant filamentous fungus. Typical yield is 0.56 g biomass per g COD utilized. The amino acid profile of the biomass compares well with other edible protein sources. It was possible to reduce the COD concentration in the effluent from ± 8000 mg/L to ± 150 mg/L.


2020 ◽  
Vol 24 (3) ◽  
pp. 457-469
Author(s):  
Kriss Spalvins ◽  
Zane Geiba ◽  
Zane Kusnere ◽  
Dagnija Blumberga

AbstractCooking oils are widely used in food preparation. During cooking, harmful compounds are formed in oils, therefore utilization of used cooking oils (waste cooking oils) is limited. Single cell protein (SCP) is dietary protein, which can be produced from various protein-rich microorganisms that are capable of utilizing industrial by-products such as waste cooking oil (WCO). In this study the utilization of industrial WCO (obtained from local potato chips manufacturer) as a carbon source for single cell protein production by yeast Yarrowia lipolytica was assessed. The medium containing 27.5 g/L WCO and C/N ratio of 5–10 for batch fermentations was determined to be the optimal composition for SCP production. In this study, the highest reported Yarrowia lipolytica biomass concentration (57.37 g/L) was achieved when WCO was used as the main carbon source. Protein concentrations were relatively low (12.6 %), which also affected the final protein yield (7.23 g/L). The resulting biomass accumulated low concentrations of toxic malondialdehyde (MDA) (2.32 mg MDA/kg) compared to concentrations initially detected in the WCO itself (30.87 mg MDA/kg). To the best of the authors knowledge this is the first study to report on MDA decrease via microbial fermentations.


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