scholarly journals Addition of Carbon Sources to Pineapple Waste Media in the Production of Single Cell Protein Biomass Saccharomyces cerevisiae

2020 ◽  
Vol 2 (2) ◽  
pp. 70
Author(s):  
Anggi Nurmalasari ◽  
Shinta Maharani
Keyword(s):  
Single Cell ◽  
Protein Content ◽  
Carbon Sources ◽  
Dry Weight ◽  
Fermentation Medium ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Fermentation Time ◽  
Pineapple Peel ◽  
The Media

Single-cell protein (SCP) is the term used for crude or pure protein derived from simple single or multi-celled microorganisms. Pineapple peel contains monosaccharides as much as 10.8% so that it can be used as a fermentation medium in single-cell protein production. The purpose of this study was to determine the effect of adding carbon sources of fructose and sucrose on pH, cell dry weight, and protein content in the manufacture of single-cell proteins. This study used a completely randomized design (CRD) with two factors, namely the addition of carbon (fructose, sucrose, and control) and fermentation time (24.48, and 72 hours). The data analysis used the variance test and the Duncan Multiple Range Test (DMRT) continued to test with a confidence level of 95%. The results showed that the addition of carbon to the media had a very significant effect on media pH, cell dry weight, and protein content. In the medium with the addition of fructose it has a pH of 3.81; dry weight 0.4203 grams; and protein content 69.08/L. Whereas in the media with the addition of sucrose, the pH was 4.33, the dry weight of the cells was 0.3385 grams, and the cells had a protein content of 85.55 mg/L. The addition of a fructose carbon source gave the cell dry weight more than the addition of carbon sucrose

scholarly journals Bioconversion of Single Cell Protein from Underutilised Seaweed Turbinaria sp. using Natural Palmyrah Toddy Yeast

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
S Shayanthavi ◽  
R Kapilan
Keyword(s):  
Single Cell ◽  
Crude Protein ◽  
Ph Value ◽  
Fermentation Medium ◽  
Single Cell Protein ◽  
Protein Yield ◽  
Lower Amount ◽  
Cell Protein ◽  
Fermentation Time ◽  
Initial Ph

This study was aimed to produce Single Cell Protein (SCP) from underutilied, abundantly found marine seaweed Turbinaria sp. using natural yeast from palmyrah (Borassus flabellifer) toddy. Turbinaria sp. were collected, sun-dried, ground to powder form and Turbinaria solution was made by mixing distilled water and this was used as a growth medium throughout the experiment without adding any supplements. The experiments were performed in a shaking incubator with the fermentation medium of Turbinaria sp. 100 g/L (10%) inoculated with 10 mL natural palmyrah toddy. Fermentation was carried out at 29o C for 72 h and the crude protein content was determined using Kjeldahl apparatus (38.46%). When the growth temperature was optimised at 35o C, crude protein yield obtaine was significantly increased by 1.36 times than the initial non-optimised condition with temperature 27oC. When fermentation time was optimised as 48 h (44.33%), crude protein yield was significantly increased by 1.14 times than the initial fermentation time (24 h-38.55%). When different combinations of Turbinaria sp. media and inoculum ratio (55:05, 50:10, 40:20, 30:30 and 20:40) were used as medium, crude protein yield was significantly increased by 1.44 times (50:10-43.72%) than the initial media inoculum ratio (55:05-30.21%). When initial pH of the media was changed (5.0, 6.0, 7.0, 8.0 and 9.0), significantly higher relative crude protein yield was obtained with the initial pH value 6.0 (Turbinaria medium-90.97%). Amino acid analysis revealed that the SCP produced from the Turbinaria medium had all the essential amino acids with comparatively higher amount of methionine (3.9%) and lower amount of threonine (0.2%). Vitamin B analysis revealed that SCP yield from Turbinaria medium contained thiamin (0.85 mg/100 g) and riboflavin (3.2 mg/100 g). After the optimisation of culture growing conditions and media composition, SCP production from the excessively grown, underutilised seaweed Turbinaria, was significantly increased by 1.13 times (from 38.46 to 43.72%) with palmyrah toddy mix. Key words: crude protein, palmyrah toddy mix, single cell protein (SCP), Turbinaria sp.

scholarly journals PRODUKSI PROTEIN SEL TUNGGAL DARI KULTUR Rhizopus oryzae DENGAN MEDIUM LIMBAH CAIR TAHU

2016 ◽  
Vol 2 (2) ◽  
pp. 132-137 ◽  
Author(s):  
Leny Maryana ◽  
Syariful Anam ◽  
Arsa Wahyu Nugrahani
Keyword(s):  
Single Cell ◽  
Protein Content ◽  
Skin Diseases ◽  
Rhizopus Oryzae ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Total Protein Content ◽  
Growth Substrate ◽  
Fermentation Time ◽  
Optimum Ph

Tofu wastewater is the origination of tofu’s production process that can degrade the quality of the environment if not handled properly. Another impact of this waste is the potential attack that could evoke the diseases such as diarrhea, skin diseases, and other illnesses for people who use the stream flow that has been polluted by this wastewater. However, the high-carbohydrate and protein of this waste may allow it to be used more as a microbial growth substrate to produce a Single-cell Protein. The purpose of this study is to determine the optimum incubation time and the highest protein content of Rhizopus oryzae in the medium of tofu wastewater based on the calculation of protein content percentage and the analysis of cell dry mass. And to know the optimum pH growth with the variation of pH growth which is about 4, 5, and 6. The calculation is performed in accordance with the fermentation time at 24, 48, and 72 hours. Total protein content calculated by the Kjeldahl method. The results showed the highest protein content obtained in the incubation period to 48 hours with an average percent of protein about 0.47%, 0.47%, 0.46% and a mass of cell with an average value of 0.77%, 0, 84% and 0.91%. The optimum pH of Rhizopus oryzae growth in the medium of tofu wastewater is about 5 pH. It can be concluded that the wastewater of tofu potentially used as a growth substrate of Rhizopus oryzae. Keywords: Wastewater of Tofu, Single-cell Protein, Rhizopus oryzae.

scholarly journals Effect of Land Agricultural Fertilizer on Growth of Marine Single Cell Protein, Spirulina platensis, Chlorella vulgaris and Nannochloropsis

Omni-Akuatika ◽  
2019 ◽  
Vol 15 (2) ◽  
pp. 69
Author(s):  
Purnama Sukardi ◽  
Ferisa Wahyunika ◽  
Tjahyo Winanto ◽  
Norman Arie Prayogo ◽  
Taufan Harisam ◽  
...  
Keyword(s):  
Growth Rate ◽  
Single Cell ◽  
Protein Content ◽  
Chlorella Vulgaris ◽  
Spirulina Platensis ◽  
Single Cell Protein ◽  
Nannochloropsis Oculata ◽  
Cell Protein ◽  
C 30 ◽  
Agricultural Fertilizer

This study aimed to determine the growth rate and protein content in Nannochloropsis oculata, Chlorella vulgaris, Spirulina platensis were cultured using agricultural fertilizers. The agricultural fertilizers used were Urea, ZA and TSP. Each single cell protein was cultured using the three types of fertilizers with a ratio of Urea: ZA and TSP as follows (A) 1: 2: 1 [(10:20:10 g / L)], (B) 2: 2: 1 [(20:20:10 g / L)] and (C) 3: 2: 1 [(30:20:10 g / L)], respectively. The results showed that the best ratio of Urea, ZA and TSP fertilizers to growth of Spirulina, Nannochloropsis oculata and Chlorella vulgaris was C [30:20:10 (g / L)] treatment. However, phyto-protein content in Nannochlorophsis differed significantly between fertilization treatments, C fertilization yielded the highest protein content (28.75±0.05%), when compared with A (25.13±0.01%) and B (25.14±0.02%), respectively. In Chlorella vulgaris, all fertilization treatments showed very significant differences, B fertilization (28.24%±00.006) yielded the highest phyto-protein content, if compared to A (23.63% ± 0.003) and C (19.74% ±0.006 ), respectively. All fertilization treatments showed very significant differences (P <0.05) on Spirulina platensis. The highest content of phyto-protein (62.68 + 0.05%) was present in treatment C, when compared to A (52.18 + 0.05%) and B (62.37 + 0.01%) treatments, respectively.

scholarly journals Bioconversion of Saw Dust Powder Acid Hydrolysis to Single Cell Protein by the Yeast Candida tropicalis

2021 ◽  
Vol 2 (02) ◽  
pp. 31-35
Author(s):  
Mustafa Haider
Keyword(s):  
Carbon Source ◽  
Single Cell ◽  
Candida Tropicalis ◽  
Basal Medium ◽  
Ammonium Phosphate ◽  
Dry Weight ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Low Grade ◽  
Saw Dust

This study was aimed to grow the local isolates yeast Candida tropicalis on locally prepared sawdust powder hydrolysate as a basal medium and carbon source with respect to single cell protein (SCP) production. The saw dust powder was treated with 10% H2SO4 for one hour at 100 0C. After cooling the supernatant containing the isolated sugar separated from debris by filtration and used as a carbon source and basal medium for yeast growth and SCP production. A high amount of SCP was achieved after five days of incubation, the percentage of the produced SCP 39.05% of the biomass dry weight, which is equivalent to 3.07g/l. SCP formation biosynthesis was affected by the level of nitrogen present in the medium; a high amount of protein being achieved in fermentation medium containing 0.3% urea in which the percentage of the yielded SCP was increased to 42.54%, (3.71g/l) of the biomass dry weight. The effect of varying nitrogen sources on SCP accumulation was also assayed. Medium containing ammonium phosphate greatly stimulated protein production in which the produced SCP was increased to reach 5.90 g/l. The produced amount equivalent to (48.22%) of the biomass dry weight. Conversely, a medium containing sodium nitrate had a suppressive effect on SCP production. This evidence clearly suggests that the metabolic versatility of Candida tropicalis may be employed in the conversion of low-grade material into high SCP product.

scholarly journals SOLIDS REDUCTION AND MICROBIAL PROTEIN MEASUREMENT IN BIODIGESTER USED IN CASSAVA WASTEWATER TREATMENT

Engevista ◽  
2016 ◽  
Vol 18 (2) ◽  
pp. 329
Author(s):  
Jussara Silva Berger ◽  
Eliane Hermes ◽  
Dilcemara Cristina Zenatti ◽  
Manoel Penachio Gonçalves ◽  
Marcos Araujo Lins ◽  
...  
Keyword(s):  
Protein Content ◽  
Crude Protein ◽  
Dry Weight ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Total Protein Content ◽  
Microbial Protein ◽  
Volatile Solids ◽  
Cassava Wastewater ◽  
Solids Removal

This study aimed at evaluating the efficiency of solids removal and quantifying microbial protein in residual biomass from a digester used to treat cassava effluent. The effluent was evaluated in the inlet and outlet to determine its effectiveness, but, in order to measure microbial protein, only the digester output was used. The total protein content in dry biomass was determined by Kjeldahl method, adopting the factor of 6.25 for the conversion from the total nitrogen. Was determined dry weight and quantified the protein content present in the same. Turbidity, total solids and volatile solids were removed in 73.97, 64.37 and 76.04%, respectively. The average dry weight was 2.1 kg m3 with almost 54.73% crude protein. The average effluent flow of cassava was considered as 90 m3 h-1; thus, average crude protein production was estimated in 124 kg h-1. According to the obtained results, the improvement and optimization of the process are aimed to produce a single-cell protein with food quality.

Utilization of Potato-Corn Biosolids Single-Cell Protein and Potato-Corn Primary Waste by Beef Cattle2

1984 ◽  
Vol 58 (5) ◽  
pp. 1292-1299 ◽  
Author(s):  
J. C. Hsu ◽  
T. W. Perry ◽  
M. T. Mohler
Keyword(s):  
Single Cell ◽  
Single Cell Protein ◽  
Cell Protein

Comparative Study on Single Cell Protein (SCP) Production by Trichoderma Viride From Pineapple Wastes and Banana Peels

2019 ◽  
Author(s):  
Chioma Ogugua Anichebe ◽  
Uba Bright O ◽  
Ebele Linda Okoye ◽  
Charles C. Onochie
Keyword(s):  
Comparative Study ◽  
Single Cell ◽  
Trichoderma Viride ◽  
Single Cell Protein ◽  
Cell Protein

Single cell protein as food and feed

Nahrung/Food ◽  
1988 ◽  
Vol 32 (3) ◽  
pp. 219-229 ◽  
Author(s):  
A. Giec ◽  
J. Skupin
Keyword(s):  
Single Cell ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Food And Feed
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