Antifungal activity of plant extract of Ephedra alata sub.sp. alenda (Stapf) Trab., on mycelial growth of Fusarium oxysporum f. sp. albedi-nis, the causal agent of Bayoud disease on date palm (Phoenix dactylifera L.)

2020 ◽  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Plant Extract ◽  
Mycelial Growth ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Causal Agent ◽  
Phoenix Dactylifera L ◽  
Bayoud Disease

scholarly journals Exploring the potential of using bioactive plant products in the management of Fusarium oxysporum f.sp. albedinis: the causal agent of Bayoud disease on date palm (Phoenix dactylifera L.)

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Eimad Dine Tariq Bouhlali ◽  
Mgal Derouich ◽  
Houria Ben-Amar ◽  
Reda Meziani ◽  
Adil Essarioui
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Plant Extracts ◽  
Date Palm ◽  
Antioxidant Properties ◽  
Phoenix Dactylifera ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Dependent Manner ◽  
Antioxidant Power ◽  
Bayoud Disease

Abstract Background “Bayoud” disease caused by Fusarium oxysporum f. sp. albedinis (Foa) poses a serious threat to date palm (Phoenix dactylifera L.) in Morocco. However, research studies performed to discover biological methods to control this disease remain limited. The present study has set objectives to determine antifungal activity of five plants extracts (Acacia cyanophylla, Cupressus atlantica, Eucalyptus torquata, Nerium oleander, and Schinus molle) against Foa and link this effect to their content in polyphenols and flavonoids as well as their antioxidant properties. Results Plant extracts showed significant differences (p < 0.05) regarding their antifungal activity. The extracts of E. torquata and C. atlantica showed the strongest antifungal effect resulting in the inhibition of mycelial growth, sporulation, and spore germination in a dose-dependent manner. In addition, there were significant differences among the examined plant extracts in respect to their total polyphenols (1.536–7.348 g GAE/100 g DW), flavonoids (0.986–5.759 g RE/100 g DW), and antioxidant properties measured by Trolox equivalent antioxidant capacity (TEAC) (7.47–38.97 mmol TE/100 g DW) and ferric-reducing antioxidant power (FRAP) assay (8.95–47.36 mmol TE/100 g DW). Moreover, the antifungal potential of plant extracts was found to be moderately to strongly correlated with their polyphenol and flavonoid contents as well as their antioxidant activity, implying that the effective inhibitory activity of these plant extracts is partly due to their richness in antioxidative secondary metabolites. Conclusion Our findings shed further light on plants as a-yet-untapped resource of bioactive compounds and constructed the foundation for the development of new biological approaches to best manage Bayoud disease.

scholarly journals Biocontrol of Bayoud Disease (Fusarium oxysporum F. Sp. Albedinis) on Deglet-nour Variety of Date Palm (Phoenix dactylifera L.) in South Western Oases of Algeria

2021 ◽  
Author(s):  
Hakima Belaidi ◽  
Fawzia Toumi-Benali ◽  
Ibrahim Elkhalil Benzohra
Keyword(s):  
Fusarium Oxysporum ◽  
Date Palm ◽  
Total Mortality ◽  
Phoenix Dactylifera ◽  
Antagonistic Fungi ◽  
Palm Trees ◽  
Phoenix Dactylifera L ◽  
Complete Resistance ◽  
Bayoud Disease

Background: This work has the objective to biocontrol the bayoud disease of date palm caused by the fungus Fusarium oxysporum f. sp. albedinis (Foa), is the major disease in Algerian palm groves of south western region. This in vivo biocontrol was done on date palm variety Deglet-Nour, using two antagonistic fungi species, Trichoderma harzianum and Aspergillus flavus.Methods: Twenty Foa isolates obtained from isolation of the spines carrying the typical symptoms of Bayoud disease were used. Two strains T. harzianum and A. flavus, were isolated from rhizosphere soils of the date palm trees. Using the greenhouse screening test, which was carried out on 3-4 month-old date palm seedlings at the rate of 5 ml of inoculum suspension.Result: A statistical analysis showed a significant (P less than 0.05), difference of Deglet-Nour seedlings reactions against Foa isolates and antagonistic fungi were observed. All date palm trees present susceptibility against Foa until the total mortality of seedlings. The biocontrol test showed that two antagonists showed different reaction, with the complete resistance for seedlings treated by T. harzianum with the mortality rate (rm%) reduced at 100%, while, the second antagonist A. flavus reduced the rm% at 25%. We can apply of these antagonistic fungi to protect our groves contaminated by Bayoud disease and also contain this susceptible commercial variety.

scholarly journals Antifungal activity of Acacia tortilis subsp. raddiana tar on Fusarium oxysporum f.sp. albedinis, the cause of Bayoud disease of the date palm in Southwest Algeria

2019 ◽  
Author(s):  
A. Mezouari ◽  
A. Makhloufi ◽  
K. Bendjima ◽  
L. Benlarbi ◽  
A. Boulanouar ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Date Palm ◽  
Refraction Index ◽  
Phoenix Dactylifera ◽  
Effective Control ◽  
Pcr Analysis ◽  
Acacia Tortilis ◽  
Species Specific

Bayoud caused by Fusarium oxysporum f.sp. albedinis (Foa), is the most destructive disease of the date palm (Phoenix dactylifera L.) in Morocco and Algeria and there is no effective control strategy. We found that although Foa isolates vary morphologically, Foa strains can be identified by species-specific primers. PCR analysis revealed that the strains that we isolated from infected date palm rachis were the Bayoud pathogen Foa. We used these strains to evaluate the antifungal activity of tar extracted from Acacia tortilis subsp. raddiana. The A. raddiana tar had a density of 1.15, a refraction index of 1.3850, a pH of 5.2 and a dried matter ratio of 48.75%. The A. raddiana tar effectively inhibited the growth of Foa in vitro with a minimum inhibitory concentration of 3 µg/ml.

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