scholarly journals Characterization of Leptospira interrogans serovar Pomona isolated from swine in Brazil

2015 ◽  
Vol 9 (10) ◽  
pp. 1054-1061 ◽  
Author(s):  
Fabiana Miraglia ◽  
Luisa Z Moreno ◽  
Zenaide M Morais ◽  
Helio Langoni ◽  
Fabio H Shimabukuro ◽  
...  
Keyword(s):  
Polyclonal Antibodies ◽  
Health Hazard ◽  
Economic Loss ◽  
Molecular Techniques ◽  
Leptospira Interrogans ◽  
Serological Tests ◽  
Leptospira Interrogans Serovar Pomona ◽  
Human Health Hazard ◽  
Potential Human Health

Introduction: Leptospira interrogans swine infection is a cause of serious economic loss and a potential human health hazard. In Brazil, the most common serovars associated with swine infections are Pomona, Icterohaemorrhagie and Tarassovi. Cross-reactions among serovars and the failure of infected animals to seroconvert may complicate the interpretation of serological tests. Molecular methods with better discriminatory powers are useful tools for swine leptospirosis characterization and diagnosis. Methodology: This study evaluated nine L. interrogans isolates from the States of Sao Paulo and Minas Gerais during different time periods. Isolates from diseased and apparently healthy swine were characterized by microscopic agglutination tests with polyclonal antibodies and were genotyped by VNTR, PFGE and MLST techniques. Broth microdilution was used to determine the minimal inhibitory concentration of the antimicrobials of veterinary interest. Results: The strains were identified as L. interrogans serogroup Pomona serovar Pomona Genotype A, while MLST grouped all of the isolates in sequence type 37. The PFGE analysis resulted in two pulsotypes with more than 70% similarity, distinguishing serovar Pomona isolates from the serovar Kennewicki reference strain. All of the isolates presented low MIC values to penicillin, ampicillin, ceftiofur and tulathromycin. High MIC values for fluoroquinolones, tiamulin, gentamicin, tetracyclines, neomycin, tilmicosin and sulfas were also observed. Conclusions: All molecular techniques were concordant in L. interrogans serovar Pomona identification. This serovar may have a different antibiotic susceptibility profile than previously reported for Leptospira isolates.

Characterization of outer membrane and secreted proteins of Leptospira interrogans serovar pomona

1991 ◽  
Vol 10 (4) ◽  
pp. 311-322 ◽  
Author(s):  
Richard L. Zuerner ◽  
William Knudtson ◽  
Carole A. Bolin ◽  
Gabriel Trueba
Keyword(s):  
Outer Membrane ◽  
Secreted Proteins ◽  
Leptospira Interrogans ◽  
Leptospira Interrogans Serovar Pomona

Leishmaniasis in imported dogs in the United Kingdom; a potential human health hazard

1983 ◽  
Vol 24 (1) ◽  
pp. 23-30 ◽  
Author(s):  
J. A. LONGSTAFFE ◽  
A. R. JEFFERIES ◽  
D. F. KELLY ◽  
P. G. C. BEDFORD ◽  
M. E. HERRTAGE ◽  
...  
Keyword(s):  
United Kingdom ◽  
Human Health ◽  
Health Hazard ◽  
The United Kingdom ◽  
Human Health Hazard ◽  
Potential Human Health

Soil contamination with ascarid eggs in playgrounds in Kirikkale, Turkey

2006 ◽  
Vol 80 (1) ◽  
pp. 15-18 ◽  
Author(s):  
M. Aydenizöz Özkayhan
Keyword(s):  
Soil Contamination ◽  
Human Health ◽  
Zinc Sulphate ◽  
Health Hazard ◽  
Soil Samples ◽  
Flotation Method ◽  
The City ◽  
Human Health Hazard ◽  
Potential Human Health ◽  
Toxascaris Leonina

AbstractContamination of soil with feline and canine ascarid eggs in eight playgrounds in Kırıkkale, Turkey was investigated monthly from February 2003 to January 2004. Dog faeces were also collected and all samples were examined using the zinc sulphate centrifugal flotation method. Eggs of Toxocara were observed in 5 of 8 (62.5%) of playgrounds examined and in 15.6% of 480 soil samples. The number of eggs varied from 1 to 11. Eggs were observed in soil samples collected in February, March to June, August and November, with embryonated eggs appearing in June and August. Eggs of Toxascaris leonina and Taenia spp. and oocysts of Isospora spp. were also found in 1.5%, 1.0% and 0.2% of soil samples, respectively. Of 26 samples of dog faeces collected, 7.7% were contaminated with Toxocara spp. and 11.5% with Taenia spp. The presence of Toxocara eggs in the city playgrounds and dog populations suggests a potential human health hazard due to toxocariasis.

scholarly journals Characterization of Ocular Surface Microbial Profiles Revealed Discrepancies between Conjunctival and Corneal Microbiota

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 405
Author(s):  
Anna Matysiak ◽  
Michal Kabza ◽  
Justyna A. Karolak ◽  
Marcelina M. Jaworska ◽  
Malgorzata Rydzanicz ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Ocular Surface ◽  
Molecular Techniques ◽  
Corneal Tissue ◽  
Rna Seq ◽  
Microbiome Composition ◽  
Viral Sequences ◽  
Pcr Techniques ◽  
Unmapped Reads

The ocular microbiome composition has only been partially characterized. Here, we used RNA-sequencing (RNA-Seq) data to assess microbial diversity in human corneal tissue. Additionally, conjunctival swab samples were examined to characterize ocular surface microbiota. Short RNA-Seq reads, obtained from a previous transcriptome study of 50 corneal tissues, were mapped to the human reference genome GRCh38 to remove sequences of human origin. The unmapped reads were then used for taxonomic classification by comparing them with known bacterial, archaeal, and viral sequences from public databases. The components of microbial communities were identified and characterized using both conventional microbiology and polymerase chain reaction (PCR) techniques in 36 conjunctival swabs. The majority of ocular samples examined by conventional and molecular techniques showed very similar microbial taxonomic profiles, with most of the microorganisms being classified into Proteobacteria, Firmicutes, and Actinobacteria phyla. Only 50% of conjunctival samples exhibited bacterial growth. The PCR detection provided a broader overview of positive results for conjunctival materials. The RNA-Seq assessment revealed significant variability of the corneal microbial communities, including fastidious bacteria and viruses. The use of the combined techniques allowed for a comprehensive characterization of the eye microbiome’s elements, especially in aspects of microbiota diversity.

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