scholarly journals Lactobacillus strains isolated from oysters improve the production of Crassostrea gigas larvae

2021 ◽  
Vol 49 (4) ◽  
pp. 551-564
Author(s):  
Macario Savin-Amador ◽  
Maurilia Rojas-Contreras ◽  
Pablo Misael Arce-Amézquita ◽  
Carlos Rangel-Dávalos ◽  
Ricardo Vázquez-Juárez
Keyword(s):  
Crassostrea Gigas ◽  
Larval Settlement ◽  
Isochrysis Galbana ◽  
Pathogenic Microorganisms ◽  
Physiological Changes ◽  
Private Company ◽  
Probiotic Potential ◽  
Impact On Survival ◽  
Japanese Oyster ◽  
Chaetoceros Calcitrans

One of the main problems in laboratories of oyster seed production occurs in the stage of settlement or fixation and metamorphosis of the larvae. The organisms develop drastic morphological and physiological changes at this stage, exposing them to attack by pathogenic microorganisms. Numerous studies indicate that before larval settlement to a substrate, they require bacteria to stimulate and induce the process. In this study, Japanese oyster (Crassostrea gigas) larvae were fed with combinations of microalgae and bacteria previously selected for their probiotic potential; their impact on survival, growth, and settlement was evaluated. Larvae D of 12 days old were provided by a private company and transported to the laboratory under controlled conditions. Probiotic bacteria Lactobacillus plantarum 69Cr, L. fermentum 101Cc, and L. casei 43Cg were reactivated on MRS and applied at a concentration of 1×104 CFU mL-1. Microalgae Isochrysis galbana and Chaetoceros calcitrans were grown in F2 medium and supplied at a concentration of 3×104 cells mL-1. Results showed, in all treatments, a survival range of 39-53%, growth of 300-310 μm, and 34-56.5% settlement. However, the best bacteria combined with I. galbana and C. calcitrans was L. plantarum 69Cr with a 54% survival, 310 μm growth, and 56.5% settlement. It is concluded that the combination of L. plantarum 69Cr with I. galbana and C. calcitrans has the potential to be used in the production of Japanese oyster larvae.

scholarly journals Efecto de probióticos sobre el crecimiento de semillas de ostión del pacífico Crassostrea gigas

2020 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Milagro García Bernal ◽  
Ricardo Medina Marrero ◽  
Ángel Isidro Campa Córdoba ◽  
José Delfino Barajas Frías ◽  
Irasema Elizabeth Luis Villaseñor ◽  
...  
Keyword(s):  
Crassostrea Gigas ◽  
Isochrysis Galbana ◽  
Streptomyces Sp ◽  
Streptomyces Spp ◽  
Bacillus Tequilensis ◽  
Bacillus Endophyticus ◽  
Chaetoceros Calcitrans

 Se  realizó  un  estudio  en  juveniles  de  Crassostrea  gigas  para investigar el potencial efecto  probiótico de Streptomyces  spp. (cepas V4, N7 y RL8) y de una mezcla de Bacillus (BMix) teniendo como variable de respuesta su  crecimiento.  Las  bacterias  fueron  adicionadas  con  el  alimento  vivo (microalgas),  para  ser  ingeridas  por  los  ostiones  mediante  filtración.  El  diseño experimental  incluyó  cuatro  tratamientos  bacterianos:  [T1  (Streptomyces  sp. V4); T2 (Streptomyces sp. N7); T3 (Streptomyces sp. RL8); T4, una mezcla de bacilos  BMix  (Bacillus  tequilensis  YC5-2,  Bacillus  endophyticus  C2-2  y Bacillus  endophyticus  YC3-B]  y  un  tratamiento  Control  (T5)  con  solo microalgas  como  alimento.  Estas  cepas  potencialmente  probióticas  fueron suministradas a una concentración final de 1 × 106 UFC mL-1 y las microalgas (Isochrysis galbana y Chaetoceros calcitrans en proporción 1:1) a una densidad de 70-80 × 103 células mL-1). Los tratamientos T1 y T3 (Streptomyces spp. V4 y RL8,  respectivamente),  incrementaron  significativamente  el  área  y  el  diámetro teórico de la concha de C. gigas. Esto sugiere que la adición Strepromyces spp. V4  y  RL8  puede  mejorar  el  crecimiento  de  C.  gigas  durante  el  proceso  de preengorda de semillas en laboratorio.   

scholarly journals Effect of microalgal diets on filtration and ingestion rates of the rotifer Brachionus plicatilis

2016 ◽  
Vol 63 (3) ◽  
Author(s):  
Jayasree Loka ◽  
K.K. Philipose ◽  
S.M. Sonali
Keyword(s):  
Filtration Rate ◽  
Brachionus Plicatilis ◽  
Ingestion Rate ◽  
Initial Density ◽  
Isochrysis Galbana ◽  
Nannochloropsis Oculata ◽  
Ingestion Rates ◽  
Significant Difference ◽  
Rotifer Brachionus Plicatilis ◽  
Chaetoceros Calcitrans

Effect of marine microalgal diet on filtration and ingestion rates of Brachionus plicatilis was studied. Marine microalgae viz., Nannochloropsis oculata, Isochrysis galbana, Chaetoceros calcitrans and a combination of N. oculata and I. galbana (Nanno+Iso) at different cell concentrations were selected for the experiment and triplicates were maintained for each feed. Significant difference (p<0.05) was observed in the filtration and ingestion rates of B. plicatilis, between the treatments. Peak filtration rate of 12.2x10-5 cells ml-1 ind-1 min-1 was recorded in B. plicatilis fed with Nanno+Iso, followed by I. galbana. Ingestion rate was found to be significantly high (p<0.05) for those fed with C. calcitrans, followed by Nanno+Iso. Filtration rate was significantly high (p<0.05) in rotifers stocked at an initial density of 50 nos. ml-1 fed with Nanno+Iso. The results indicated that a combination of Nanno and Iso is the best suitable microalgal diet for rotifer with peak filtration (12.2x10-5cells ml-1 ind-1 min-1) and ingestion (5.4x10-3 cells ml-1 ind-1 min-1) rates during the first 60 min.

Pathological changes by Eosinophilic Rickettsia-like organism in Japanese oyster, Crassostrea gigas

2019 ◽  
Vol 167 ◽  
pp. 107248
Author(s):  
Liliana Carvalho-Saucedo ◽  
Ilie S. Racotta ◽  
Citlali Guerra-Danielsen
Keyword(s):  
Crassostrea Gigas ◽  
Pathological Changes ◽  
Japanese Oyster

A population dynamics model for the Japanese oyster, Crassostrea gigas

Aquaculture ◽  
1997 ◽  
Vol 149 (3-4) ◽  
pp. 285-321 ◽  
Author(s):  
Masato Kobayashi ◽  
Eileen E Hofmann ◽  
Eric N Powell ◽  
John M Klinck ◽  
Koji Kusaka
Keyword(s):  
Population Dynamics ◽  
Crassostrea Gigas ◽  
Dynamics Model ◽  
Population Dynamics Model ◽  
Japanese Oyster

Cryopreservation of spermatozoa of the Japanese oyster, Crassostrea gigas

Aquaculture ◽  
1986 ◽  
Vol 58 (3-4) ◽  
pp. 277-280 ◽  
Author(s):  
S. Bougrier ◽  
L.D. Rabenomanana
Keyword(s):  
Crassostrea Gigas ◽  
Japanese Oyster

Overdominance of a Muscle Protein (Mp-1) Locus in the Japanese Oyster, Crassostrea gigas (Ostreidae)

1979 ◽  
Vol 36 (11) ◽  
pp. 1313-1318 ◽  
Author(s):  
N. E. Buroker
Keyword(s):  
Marine Environment ◽  
Crassostrea Gigas ◽  
Genetic Study ◽  
Thermal Gradient ◽  
Population Genetic ◽  
Muscle Protein ◽  
Selective Advantage ◽  
Relative Fitness ◽  
Population Genetic Study ◽  
Japanese Oyster

A microgeographical population genetic study of the Japanese oyster, Crassostrea gigas, revealed a case of overdominance for a muscle protein (Mp-1) locus. This locus consists of a two allele polymorphism. A study of the relative "fitness" of the three genotypes (Mp-1105/105, Mp-1105/100, and Mp-1100/100) revealed a heterozygote superiority in the segment of the oyster population at the lowest tidal level (+ 1.2 m). In segments of the population at higher tidal heights (i.e. + 1.8 and + 2.4 m), there appears to be a selective advantage for Mp-1105 allele since the highest "fitness" values belong to the Mp-1105/105 genotype. Since oysters do not appear at the field station lower than 1.2 tidal m, it could not be determined if the Mp-1100 allele had a selective advantage at tidal levels lower than 1.2 m. However, the Mp-1105 allele possibly exhibits a selective advantage in the subtropical marine environment of southern Japan, while the Mp-1100 allele is favored in the temperate marine environment of northern Japan. This suggests perhaps that a micro- and macrogeographical cline in gene frequency exists for the Mp-1 locus due to a thermal gradient. Key words: Japanese oyster, Crassostrea gigas, population genetic study, overdominance, heterozygote superiority, Mp-1 locus, micro- and macrogeographical cline, thermal gradient, tidal height, latitude

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