scholarly journals Efecto de probióticos sobre el crecimiento de semillas de ostión del pacífico Crassostrea gigas

2020 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Milagro García Bernal ◽  
Ricardo Medina Marrero ◽  
Ángel Isidro Campa Córdoba ◽  
José Delfino Barajas Frías ◽  
Irasema Elizabeth Luis Villaseñor ◽  
...  
Keyword(s):  
Crassostrea Gigas ◽  
Isochrysis Galbana ◽  
Streptomyces Sp ◽  
Streptomyces Spp ◽  
Bacillus Tequilensis ◽  
Bacillus Endophyticus ◽  
Chaetoceros Calcitrans

 Se  realizó  un  estudio  en  juveniles  de  Crassostrea  gigas  para investigar el potencial efecto  probiótico de Streptomyces  spp. (cepas V4, N7 y RL8) y de una mezcla de Bacillus (BMix) teniendo como variable de respuesta su  crecimiento.  Las  bacterias  fueron  adicionadas  con  el  alimento  vivo (microalgas),  para  ser  ingeridas  por  los  ostiones  mediante  filtración.  El  diseño experimental  incluyó  cuatro  tratamientos  bacterianos:  [T1  (Streptomyces  sp. V4); T2 (Streptomyces sp. N7); T3 (Streptomyces sp. RL8); T4, una mezcla de bacilos  BMix  (Bacillus  tequilensis  YC5-2,  Bacillus  endophyticus  C2-2  y Bacillus  endophyticus  YC3-B]  y  un  tratamiento  Control  (T5)  con  solo microalgas  como  alimento.  Estas  cepas  potencialmente  probióticas  fueron suministradas a una concentración final de 1 × 106 UFC mL-1 y las microalgas (Isochrysis galbana y Chaetoceros calcitrans en proporción 1:1) a una densidad de 70-80 × 103 células mL-1). Los tratamientos T1 y T3 (Streptomyces spp. V4 y RL8,  respectivamente),  incrementaron  significativamente  el  área  y  el  diámetro teórico de la concha de C. gigas. Esto sugiere que la adición Strepromyces spp. V4  y  RL8  puede  mejorar  el  crecimiento  de  C.  gigas  durante  el  proceso  de preengorda de semillas en laboratorio.   

scholarly journals Lactobacillus strains isolated from oysters improve the production of Crassostrea gigas larvae

2021 ◽  
Vol 49 (4) ◽  
pp. 551-564
Author(s):  
Macario Savin-Amador ◽  
Maurilia Rojas-Contreras ◽  
Pablo Misael Arce-Amézquita ◽  
Carlos Rangel-Dávalos ◽  
Ricardo Vázquez-Juárez
Keyword(s):  
Crassostrea Gigas ◽  
Larval Settlement ◽  
Isochrysis Galbana ◽  
Pathogenic Microorganisms ◽  
Physiological Changes ◽  
Private Company ◽  
Probiotic Potential ◽  
Impact On Survival ◽  
Japanese Oyster ◽  
Chaetoceros Calcitrans

One of the main problems in laboratories of oyster seed production occurs in the stage of settlement or fixation and metamorphosis of the larvae. The organisms develop drastic morphological and physiological changes at this stage, exposing them to attack by pathogenic microorganisms. Numerous studies indicate that before larval settlement to a substrate, they require bacteria to stimulate and induce the process. In this study, Japanese oyster (Crassostrea gigas) larvae were fed with combinations of microalgae and bacteria previously selected for their probiotic potential; their impact on survival, growth, and settlement was evaluated. Larvae D of 12 days old were provided by a private company and transported to the laboratory under controlled conditions. Probiotic bacteria Lactobacillus plantarum 69Cr, L. fermentum 101Cc, and L. casei 43Cg were reactivated on MRS and applied at a concentration of 1×104 CFU mL-1. Microalgae Isochrysis galbana and Chaetoceros calcitrans were grown in F2 medium and supplied at a concentration of 3×104 cells mL-1. Results showed, in all treatments, a survival range of 39-53%, growth of 300-310 μm, and 34-56.5% settlement. However, the best bacteria combined with I. galbana and C. calcitrans was L. plantarum 69Cr with a 54% survival, 310 μm growth, and 56.5% settlement. It is concluded that the combination of L. plantarum 69Cr with I. galbana and C. calcitrans has the potential to be used in the production of Japanese oyster larvae.

scholarly journals Effect of microalgal diets on filtration and ingestion rates of the rotifer Brachionus plicatilis

2016 ◽  
Vol 63 (3) ◽  
Author(s):  
Jayasree Loka ◽  
K.K. Philipose ◽  
S.M. Sonali
Keyword(s):  
Filtration Rate ◽  
Brachionus Plicatilis ◽  
Ingestion Rate ◽  
Initial Density ◽  
Isochrysis Galbana ◽  
Nannochloropsis Oculata ◽  
Ingestion Rates ◽  
Significant Difference ◽  
Rotifer Brachionus Plicatilis ◽  
Chaetoceros Calcitrans

Effect of marine microalgal diet on filtration and ingestion rates of Brachionus plicatilis was studied. Marine microalgae viz., Nannochloropsis oculata, Isochrysis galbana, Chaetoceros calcitrans and a combination of N. oculata and I. galbana (Nanno+Iso) at different cell concentrations were selected for the experiment and triplicates were maintained for each feed. Significant difference (p<0.05) was observed in the filtration and ingestion rates of B. plicatilis, between the treatments. Peak filtration rate of 12.2x10-5 cells ml-1 ind-1 min-1 was recorded in B. plicatilis fed with Nanno+Iso, followed by I. galbana. Ingestion rate was found to be significantly high (p<0.05) for those fed with C. calcitrans, followed by Nanno+Iso. Filtration rate was significantly high (p<0.05) in rotifers stocked at an initial density of 50 nos. ml-1 fed with Nanno+Iso. The results indicated that a combination of Nanno and Iso is the best suitable microalgal diet for rotifer with peak filtration (12.2x10-5cells ml-1 ind-1 min-1) and ingestion (5.4x10-3 cells ml-1 ind-1 min-1) rates during the first 60 min.

scholarly journals Promiscuous Pathogenicity Islands and Phylogeny of Pathogenic Streptomyces spp.

2016 ◽  
Vol 29 (8) ◽  
pp. 640-650 ◽  
Author(s):  
Yucheng Zhang ◽  
Dawn R. D. Bignell ◽  
Ran Zuo ◽  
Qiurong Fan ◽  
Jose C. Huguet-Tapia ◽  
...  
Keyword(s):  
Potato Scab ◽  
Pathogenic Species ◽  
Genome Sequences ◽  
Streptomyces Species ◽  
Streptomyces Sp ◽  
Streptomyces Spp ◽  
Streptomyces Turgidiscabies ◽  
Streptomyces Diastatochromogenes ◽  
Pathogenicity Determinant

Approximately 10 Streptomyces species cause disease on underground plant structures. The most economically important of these is potato scab, and the most studied of these pathogens is Streptomyces scabiei (syn. S. scabies). The main pathogenicity determinant of scab-causing Streptomyces species is a nitrated diketopiperazine, known as thaxtomin A (ThxA). In the pathogenic species Streptomyces turgidiscabies, ThxA biosynthetic genes reside on a mobile pathogenicity island (PAI). However, the mobilization of PAIs in other Streptomyces species remains uncharacterized. Here, we investigated the mobilization of the PAI of S. scabiei 87-22. Based on whole genome sequences, we inferred the evolutionary relationships of pathogenic Streptomyces species and discovered that Streptomyces sp. strain 96-12, a novel pathogenic species isolated from potatoes in Egypt, was phylogenetically grouped with nonpathogenic species rather than with known pathogenic species. We also found that Streptomyces sp. strain 96-12 contains a PAI that is almost identical to the PAI in S. scabiei 87-22, despite significant differences in their genome sequences. This suggested direct or indirect in vivo mobilization of the PAI between S. scabiei and nonpathogenic Streptomyces species. To test whether the S. scabiei 87-22 PAI could, indeed, be mobilized, S. scabiei 87-22 deletion mutants containing antibiotic resistance markers in the PAI were mated with Streptomyces diastatochromogenes, a nonpathogenic species. The PAI of S. scabiei was site-specifically inserted into the aviX1 gene of S. diastatochromogenes and conferred pathogenicity in radish seedling assays. Our results demonstrated that S. scabiei, the earliest described Streptomyces pathogen, could be the source of a PAI responsible for the emergence of novel pathogenic species.

scholarly journals Interactions between Filter-Feeding Bivalves and Toxic Diatoms: Influence on the Feeding Behavior of Crassostrea gigas and Pecten maximus and on Toxin Production by Pseudo-nitzschia

Toxins ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 577
Author(s):  
Aurore Sauvey ◽  
Françoise Denis ◽  
Hélène Hégaret ◽  
Bertrand Le Roy ◽  
Christophe Lelong ◽  
...  
Keyword(s):  
Crassostrea Gigas ◽  
Domoic Acid ◽  
Diet Composition ◽  
Isochrysis Galbana ◽  
Toxin Production ◽  
Pecten Maximus ◽  
Bivalve Species ◽  
Feeding Rates ◽  
Filter Feeding ◽  
Complex Interactions

Among Pseudo-nitzschia species, some produce the neurotoxin domoic acid (DA), a source of serious health problems for marine organisms. Filter-feeding organisms—e.g., bivalves feeding on toxigenic Pseudo-nitzschia spp.—are the main vector of DA in humans. However, little is known about the interactions between bivalves and Pseudo-nitzschia. In this study, we examined the interactions between two juvenile bivalve species—oyster (Crassostrea gigas) and scallop (Pecten maximus)—and two toxic Pseudo-nitzschia species—P. australis and P. fraudulenta. We characterized the influence of (1) diet composition and the Pseudo-nitzschia DA content on the feeding rates of oysters and scallops, and (2) the presence of bivalves on Pseudo-nitzschia toxin production. Both bivalve species fed on P. australis and P. fraudulenta. However, they preferentially filtered the non-toxic Isochrysis galbana compared to Pseudo-nitzschia. The presence of the most toxic P. australis species resulted in a decreased clearance rate in C. gigas. The two bivalve species accumulated DA in their tissues (up to 0.35 × 10−3 and 5.1 × 10−3 µg g−1 for C. gigas and P. maximus, respectively). Most importantly, the presence of bivalves induced an increase in the cellular DA contents of both Pseudo-nitzschia species (up to 58-fold in P. fraudulenta in the presence of C. gigas). This is the first evidence of DA production by Pseudo-nitzschia species stimulated in the presence of filter-feeding bivalves. The results of this study highlight complex interactions that can influence toxin production by Pseudo-nitzschia and accumulation in bivalves. These results will help to better understand the biotic factors that drive DA production by Pseudo-nitzschia and bivalve contamination during Pseudo-nitzschia blooms.

scholarly journals The result of effective microorganisms isolated from wastewater and soil

2021 ◽  
Vol 32 (1) ◽  
pp. 38-44
Author(s):  
Oyunbileg Natsagdorj ◽  
Indra Nyamjav ◽  
Byambasuren Davaasambuu ◽  
Ariunzaya Jargalsaikhan ◽  
Batsukh Chultem
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Heavy Metal Contamination ◽  
Biologically Active ◽  
Molecular Characteristics ◽  
Isolation And Identification ◽  
Effective Microorganisms ◽  
Streptomyces Sp ◽  
Streptomyces Spp ◽  
Rdna Sequencing ◽  
Effective Microorganism

Increasing urbanization and population concentrations are causing environmental problems such as soil and water pollution. In this study, we were present isolation and identification of Streptomyces sp and Rhodobacteria sp. Those effective microorganisms isolated from the wastewater and soil sample of Anand-Khujirt spring resort located in Khujirt soum, Uvurkhangai province in Mongolia. Based on morphological, cultural, and molecular characteristics (16S rDNA sequencing), those strain were identified as Rhodobacter sphaeroides (99% similarity) and Streptomyces cf.griseus (99% similarity). Rhodobacter sphaeroides could be used to reduce heavy metal contamination from soil. Furthermore, Streptomyces cf.griseus is an effective  microorganism, which produces 32 types of biologically active compounds for pharmaceutical or agricultural purposes. Хаягдал ус, хөрснөөс ашигтай бичил биетний цэвэр өсгөвөр ялгасан судалгааны дүнгээс Хотжилт, хүн амын төвлөрөл нэмэгдэж байгаа нь хөрс, усны бохирдол зэрэг хүрээлэн буй орчны асуудлуудыг үүсгэж байна. Бид энэхүү судалгааны ажлаар Өвөрхангай аймгийн Хужирт сумын нутагт орших “Ананд-Хужирт” рашаан сувилал,  аялал жуулчлалын баазын хаягдал ус, хөрснөөс ялгасан ашигтай бичил биетний нутгийн омгууд болох Streptomyces sp, Rhodobacteria sp-ийн нуклеотидын дарааллыг Genetyx software програмыг ашиглан боловсруулалт хийн АНУ-ын Биотехнологийн Мэдээллийн Үндэсний Төвийн мэдээллийн сангаас BLAST хайлт хийж хамгийн ойролцоох зүйлийг тодорхойлсон. Rhodobacter sphaeroides (99% ижил нуклеотидтэй)-ийг хүнд металлын бохирдлыг бууруулахад өргөн ашигладаг бол Streptomyces cf.griseus (99% ижил нуклеотидтэй) нь 32 төрлийн биологийн идэвхит нэгдлийг нийлэгжүүлэгч ашигтай бичил биетэн юм.    Түлхүүр үг: Streptomyces spp, Rhodobacteria spp, хаягдал ус, хөрс,

scholarly journals Enhancement of Cellulose Rich Organic Matter Degradation by Inoculation with Streptomyces sp. Strains

2021 ◽  
Vol 26 (02) ◽  
pp. 257-262
Author(s):  
Simonida Djuri
Keyword(s):  
Organic Matter ◽  
Extracellular Enzymes ◽  
Biochemical Characterization ◽  
Cellulose Degradation ◽  
Organic Matter Degradation ◽  
Incubation Experiment ◽  
Cellulose Production ◽  
Streptomyces Sp ◽  
Streptomyces Spp

Microbial degradation of organic matter is a vital part of carbon cycle in nature. Actinobacteria play an important role in the decomposition of cellulose rich organic matter (CROM). Streptomyces spp. are abundant in soil, produce various secondary metabolites and secrete extracellular enzymes. The aim of this research was to isolate and select Streptomyces strains with the best cellulose degradation abilities. Out of total 32 actinobacteria isolates, four Streptomyces strains (CA1, CA10, PA2 and PA7) were subjected to morphological, physiological, biochemical characterization and molecular identification. CROM degradation potential of the strains was investigated on straw and beech briquettes as well as on legume based substrate in in vitro condition. Streptomyces strains CA1 and CA10 showed the best cellulose production and starch hydrolysis abilities, followed by strains PA2 and PA7. Strain CA1 was also positive to production of pectinase enzymes. Streptomyces zaomyceticus CA1 and S. tanashiensis CA10 were used as inoculants, which degraded the raw cellulose from 38.38 to 81.69% in the investigated substrates (straw, beech, legume), during a 30-day incubation experiment. CROM inoculation with the selected Streptomyces strains improved and accelerated its degradation in controlled conditions. © 2021 Friends Science Publishers

scholarly journals Antioxidant, antimicrobial and cytotoxic activities of secondary metabolites from Streptomyces sp. isolated of the Amazon-Brazil region

2021 ◽  
Vol 10 (10) ◽  
pp. e366101018974
Author(s):  
Sandrine Maria Arruda Lima ◽  
Pedro Silvino Pereira ◽  
Bruno Iraquitan Miranda da Silva ◽  
Natalie Emanuelle Ribeiro ◽  
Elizabeth Fernanda de Oliveira Borba ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Cytotoxic Activities ◽  
Cytotoxicity Test ◽  
Peripheral Blood Mononuclear ◽  
Streptomyces Sp ◽  
Streptomyces Spp ◽  
Promising Source

Bacteria of Streptomyces genus are a promising source of biologically active products, with applications in medicine, industry and agriculture. Therefore, the objective of this work was to evaluate the cytotoxic, antioxidant and antimicrobial activities of fermented rice extract and their semipurified fractions from Streptomyces spp. isolated of the rhizosphere of Paullinia cupana, Amazon-Brazil. For this, a bioguided study was carried out by the cytotoxic activity with methanolic extract of Streptomyces sp. ACTMS-12H UFPEDA 3405 (EMeOH-12H) partitioned with n-hexane, ethyl acetate and 2-butanol. The antioxidant activity was analyzed using the DPPH, ABTS and phosphomolybdenum methods, while the antimicrobial activity was investigated by microdilution method to determine the minimum inhibitory concentration (MIC) against species of bacteria and yeast. In the cytotoxicity test, the butanolic phase (FbuOH-12H) presented IC50 of 1.1 µg/mL against MOLT-4, with cell death probably by apoptosis, but did not cause cytotoxicity on peripheral blood mononuclear cell (PBMC) or human erythrocytes. Chemical prospecting detected the presence of saponins and reducing sugars on 2-butanol fraction (FBuOH-12H), which can be related to cytotoxicity. On the antioxidant activity by ABTS, the partition with ethyl acetate (FAcOEt-12H) showed antioxidant capacity of 1161.7 ± 0.04 µM of Trolox/g of extract, indicating an expressive reactivity of the phase with this radical. The aqueous phases (from hexane, ethyl acetate and methanol extracts) were active in all tested microorganisms, except E. faecalis.

scholarly journals STUDIES ON BIOACTIVE POTENTIAL OF STREPTOMYCES SPP. (KX710212). ISOLATED FROM ENVIRONMENTAL POLLUTED SAMPLE, RANIPET, VELLORE, INDIA

2016 ◽  
Vol 9 (9) ◽  
pp. 356 ◽  
Author(s):  
N Henita Geo ◽  
Panneerselvam A
Keyword(s):  
Antibacterial Property ◽  
Scientific Evidence ◽  
Polluted Soil ◽  
Soil Samples ◽  
Potential Contribution ◽  
16S Rdna Gene ◽  
Streptomyces Sp ◽  
Streptomyces Spp ◽  
Bioactive Potential ◽  
Valuable Role

ABSTRACTObjective: Natural products from bacterial origin played and still play a valuable role in drug discovery and provide diverse health-promotingcompounds that help to cure or alleviate serious diseases.Methods: A total of 4 actinobacterial strains were isolated from 2 different environmental polluted soil samples collected from Ranipet, Vellore, TamilNadu, India. Of 15 isolates screened for antibacterial property, 4 isolates (PS1, PS2, and PS3) were found potent and characterized phenotypically andgenotypically.Results: The 16S rDNA gene sequences of potent strain showed 97% similarity with Streptomyces spp. and termed as Streptomyces sp. The bioactivitywas revealed by antibacterial and antioxidant activity.Conclusion: Hence, these findings provide scientific evidence to validate the pharmacological use and have the potential contribution in the discoveryof new lead that play a key role in the field of therapeutics.Keywords: Actinomycetes, Bioactive compounds, Antibacterial property.

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