The Polymorphism in Interleukin-6-597 G/A Gene and their Levels on Type 2 Diabetic Patients

BACKGROUND: Interleukin-6 (IL-6) pro-inflammatory cytokines play a role in the pathogenesis of inflammatory reactions in type 2 diabetes mellitus (T2DM). The -597G/A is one of IL-6 gene polymorphisms that are associated with the T2DM risk. AIM: This study aimed to observe the polymorphism in IL-6-597 G/A gene and their levels on type 2 diabetic patients at Universitas Sumatera Utara Hospital. MATERIALS AND METHODS: IL-6 -597 G/A gene polymorphisms and levels were done in 80 type 2 diabetic patients. The levels of IL-6 were performed by enzyme-linked immunosorbent assay method. Analysis of IL-6-597 G/A gene polymorphism was done using polymerase chain reaction (PCR) and restriction fragment length polymorphism. The PCR products were cut by FokI restriction enzyme and then visualized with 4% agarose. RESULTS: This study showed that the genotype frequency of GG and GA was 97.5 % and 2.5 %, respectively; however, no A/A genotype shown in this population. IL-6 levels were higher in GG genotype group compare to GA+AA genotype group, with the association were significant (p < 0.05). CONCLUSION: This study indicated that GG genotype was common genotype in the IL-6-597 G/A gene polymorphism and the polymorphism was significantly with the IL-6 levels.

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The influence of interleukin-6 gene polymorphism on omega-3 fatty acid effect in type 2 diabetic patients

Diabetes & Metabolism ◽

10.1016/j.diabet.2016.07.021 ◽

2016 ◽

Vol 42 (4) ◽

pp. 300

Author(s):

Y. Karachentsev ◽

M. Gorshunska ◽

N. Krasova ◽

E. Jansen ◽

T. Tyzhnenko ◽

...

Keyword(s):

Fatty Acid ◽

Gene Polymorphism ◽

Interleukin 6 ◽

Diabetic Patients ◽

Omega 3 ◽

Type 2 Diabetic Patients ◽

Omega 3 Fatty Acid ◽

Acid Effect ◽

Type 2 Diabetic

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Predictive Value of Osteoprotegerin for Detecting Coronary Artery Calcification in Type 2 Diabetes Mellitus Patients in Correlation with Extent of Calcification Detected by Multidetector Computed Tomography

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530319666190211122858 ◽

2019 ◽

Vol 19 (6) ◽

pp. 845-851 ◽

Cited By ~ 1

Author(s):

Sahar Ahmed ◽

Rasha Sobh

Keyword(s):

Coronary Artery ◽

Coronary Artery Calcification ◽

Enzyme Linked Immunosorbent Assay ◽

Diabetic Patients ◽

Coronary Artery Calcification Score ◽

Type 2 Diabetic Patients ◽

Duration Of Diabetes ◽

Calcification Score ◽

Type 2 Diabetic

Background:Osteoprotegerin (OPG) is a tumor necrosis factor receptor super-family member. It specifically acts on bone by increasing bone mineral density and bone volume. Recent studies have evidenced its close relation to the development of atherosclerosis and plaque destabilization. Elevated OPG level has also been associated with the degree of coronary calcification in the general population and it has been considered to be a marker of coronary atherosclerosis.Objective:The aim of this study was to determine the relation between OPG levels and Coronary Artery Calcification score (CACs) in Type 2 diabetic patients in comparison to healthy controls.Methods:Our study included 45 type 2 diabetic patients (mean age 51.7 years; 51.1% male) without evidence of previous CVD and 45 healthy age and sex matched subjects as control. All participants were subjected to full history, full examination and lab investigations. Serum OPG concentration was measured by an enzyme-linked immunosorbent assay (ELISA) and CAC imaging was performed using non contrast Multi detector CT of the heart.Results:Significant CAC (<10 Agatston units) was seen in 23 patients (51.11 %).:OPG was significantly high in diabetic patients in comparison to controls with mean 12.9±5.7 pmol/l in cases, and 8.6±0.5 pmol/l in controls (P value < 0.001).:The Coronary Artery Calcification Score (CACS) was positively correlated with age and duration of diabetes. The OPG was positively correlated with age, fasting blood sugar and duration of diabetes. The CACS showed a significantly positive correlation with OPG.Conclusion:Findings suggested that increasing in serum OPG was consistent with CAC and could be used for the early diagnosis of subclinical atherosclerosis.

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