scholarly journals In vivo high-resolution magic angle spinning proton NMR spectroscopy of Drosophila melanogaster flies as a model system to investigate mitochondrial dysfunction in Drosophila GST2 mutants

2014 ◽  
Vol 34 (1) ◽  
pp. 327-333 ◽  
Author(s):  
VALERIA RIGHI ◽  
YIORGOS APIDIANAKIS ◽  
NIKOLAOS PSYCHOGIOS ◽  
LAURENCE G. RAHME ◽  
RONALD G. TOMPKINS ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Nmr Spectroscopy ◽  
High Resolution ◽  
Mitochondrial Dysfunction ◽  
Magic Angle Spinning ◽  
Model System ◽  
Magic Angle ◽  
Proton Nmr Spectroscopy ◽  
Angle Spinning

scholarly journals Sampling Method Affects HR-MAS NMR Spectra of Healthy Caprine Brain Biopsies

Metabolites ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 38
Author(s):  
Annakatrin Häni ◽  
Gaëlle Diserens ◽  
Anna Oevermann ◽  
Peter Vermathen ◽  
Christina Precht
Keyword(s):  
Nmr Spectroscopy ◽  
Sampling Method ◽  
Magic Angle Spinning ◽  
Mas Nmr ◽  
Magic Angle ◽  
1H Nuclear Magnetic Resonance ◽  
Rapid Changes ◽  
Tissue Degradation ◽  
Angle Spinning

The metabolic profiling of tissue biopsies using high-resolution–magic angle spinning (HR-MAS) 1H nuclear magnetic resonance (NMR) spectroscopy may be influenced by experimental factors such as the sampling method. Therefore, we compared the effects of two different sampling methods on the metabolome of brain tissue obtained from the brainstem and thalamus of healthy goats by 1H HR-MAS NMR spectroscopy—in vivo-harvested biopsy by a minimally invasive stereotactic approach compared with postmortem-harvested sample by dissection with a scalpel. Lactate and creatine were elevated, and choline-containing compounds were altered in the postmortem compared to the in vivo-harvested samples, demonstrating rapid changes most likely due to sample ischemia. In addition, in the brainstem samples acetate and inositols, and in the thalamus samples ƴ-aminobutyric acid, were relatively increased postmortem, demonstrating regional differences in tissue degradation. In conclusion, in vivo-harvested brain biopsies show different metabolic alterations compared to postmortem-harvested samples, reflecting less tissue degradation. Sampling method and brain region should be taken into account in the analysis of metabolic profiles. To be as close as possible to the actual situation in the living individual, it is desirable to use brain samples obtained by stereotactic biopsy whenever possible.

scholarly journals Concentration Profiling in Rat Tissue by High-Resolution Magic-Angle Spinning NMR Spectroscopy:  Investigation of a Model Drug

2005 ◽  
Vol 77 (9) ◽  
pp. 2978-2984 ◽  
Author(s):  
Laura H. Lucas ◽  
Sarah F. Wilson ◽  
Craig E. Lunte ◽  
Cynthia K. Larive
Keyword(s):  
Nmr Spectroscopy ◽  
High Resolution ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Model Drug ◽  
Angle Spinning ◽  
Rat Tissue

High resolution triple resonance micro magic angle spinning NMR spectroscopy of nanoliter sample volumes

2016 ◽  
Vol 18 (6) ◽  
pp. 4902-4910 ◽  
Author(s):  
J. Ole Brauckmann ◽  
J. W. G. (Hans) Janssen ◽  
Arno P. M. Kentgens
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
High Resolution ◽  
Single Crystals ◽  
Solid State Nmr ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Triple Resonance ◽  
Angle Spinning

To be able to study mass-limited samples and small single crystals, a triple resonance micro-magic angle spinning (μMAS) probehead for the application of high-resolution solid-state NMR of nanoliter samples was developed.

Metabolic profile of intact tissue from uterine leiomyomas using high-resolution magic-angle-spinning 1 H NMR spectroscopy

2010 ◽  
Vol 23 (10) ◽  
pp. 1137-1145 ◽  
Author(s):  
Pierluigi Mazzei ◽  
Alessandro Piccolo ◽  
Loredana Nugnes ◽  
Massimo Mascolo ◽  
Gaetano De Rosa ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
High Resolution ◽  
Metabolic Profile ◽  
Magic Angle Spinning ◽  
Uterine Leiomyomas ◽  
Magic Angle ◽  
Intact Tissue ◽  
H Nmr ◽  
Angle Spinning
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