scholarly journals Expression profile and biological function of miR‑455‑5p in colorectal carcinoma

2018 ◽  
Author(s):  
Jinqiu Wang ◽  
Yang Lu ◽  
Yiyong Zeng ◽  
Leming Zhang ◽  
Kongliang Ke ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Expression Profile ◽  
Biological Function

scholarly journals MicroRNAs expression profile in CCR6+ regulatory T cells

2014 ◽  
Author(s):  
Juanjuan Zhao ◽  
Yongju Li ◽  
Yan Hu ◽  
Chao Chen ◽  
Ya Zhou ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Signaling Pathways ◽  
Expression Profile ◽  
Immune Cells ◽  
Potential Role ◽  
Biological Function ◽  
Fold Change ◽  
Insight Into

Backgroud: CCR6+ CD4+ regulatory T cells (CCR6+Tregs), a distinct Tregs subset, played an important role in various immune diseases. Recent evidence showed that microRNAs (miRNAs) are vital regulators in the function of immune cells. However, the potential role of miRNAs in the function of CCR6+Tregs remains largely unknown. In this study, we detected the expression profile of miRNAs in CCR6+ Tregs. Materials and Methods: The expression profile of miRNAs as well as genes in CCR6+Tregs or CCR6-Tregs from Balb/c mice were detected by microarray. The signaling pathways were analyzed using Keggs pathway library. Results: We found that there were 58 miRNAs significantly upregulated and 62 downregulated up to 2 fold in CCR6+Tregs compared with CCR6-Tregs. Moreover, 1391 genes were observed with 3 fold change and 20 signaling pathways were enriched using Keggs pathway library. Conclusion: The present data firstly showed CCR6+Tregs expressed specific miRNAs pattern, which provide an insight into the role of miRNAs in the biological function of distinct Tregs subsets.

scholarly journals MicroRNAs expression profile in CCR6+ regulatory T cells

2014 ◽  
Author(s):  
Juanjuan Zhao ◽  
Yongju Li ◽  
Yan Hu ◽  
Chao Chen ◽  
Ya Zhou ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Signaling Pathways ◽  
Expression Profile ◽  
Immune Cells ◽  
Potential Role ◽  
Biological Function ◽  
Fold Change ◽  
Insight Into

Backgroud: CCR6+ CD4+ regulatory T cells (CCR6+Tregs), a distinct Tregs subset, played an important role in various immune diseases. Recent evidence showed that microRNAs (miRNAs) are vital regulators in the function of immune cells. However, the potential role of miRNAs in the function of CCR6+Tregs remains largely unknown. In this study, we detected the expression profile of miRNAs in CCR6+ Tregs. Materials and Methods: The expression profile of miRNAs as well as genes in CCR6+Tregs or CCR6-Tregs from Balb/c mice were detected by microarray. The signaling pathways were analyzed using Keggs pathway library. Results: We found that there were 58 miRNAs significantly upregulated and 62 downregulated up to 2 fold in CCR6+Tregs compared with CCR6-Tregs. Moreover, 1391 genes were observed with 3 fold change and 20 signaling pathways were enriched using Keggs pathway library. Conclusion: The present data firstly showed CCR6+Tregs expressed specific miRNAs pattern, which provide an insight into the role of miRNAs in the biological function of distinct Tregs subsets.

scholarly journals Expression Profile of the REG Gene Family in Colorectal Carcinoma

2011 ◽  
Vol 59 (1) ◽  
pp. 106-115 ◽  
Author(s):  
Hua-chuan Zheng ◽  
Akira Sugawara ◽  
Hiroshi Okamoto ◽  
Shin Takasawa ◽  
Hiroyuki Takahashi ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Gene Family ◽  
Expression Profile

scholarly journals Most colitis associated carcinomas lack expression of LGR5: a preliminary study with implications for unique pathways of carcinogenesis compared to sporadic colorectal carcinoma

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mai Iwaya ◽  
Hiroyoshi Ota ◽  
Tomoyuki Nakajima ◽  
Takeshi Uehara ◽  
Robert Riddell ◽  
...  
Keyword(s):  
Stem Cell ◽  
Colorectal Carcinoma ◽  
Expression Profile ◽  
Interquartile Range ◽  
Intestinal Stem Cell ◽  
Cell Phenotype ◽  
Beta Catenin ◽  
Sporadic Crcs ◽  
Conventional Type ◽  
Stem Cell Phenotype

Abstract Background Leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), a component of the Wnt receptor complex, is thought to lineage label gastric and intestinal stem cells. LGR5 expression is increased in colorectal carcinoma (CRC) compared to normal tissue. Colitis associated colorectal adenocarcinoma (CAC) often shows distinct morphologic and molecular phenotypes compared to sporadic cases. However, the expression profile of LGR5, and by extension the potential role of an intestinal stem cell phenotype, has not been well described in a series of human CAC. Method RNA in situ hybridization (ISH) for LGR5 expression on 30 CACs (12 cases with conventional morphology and 18 cases with non-conventional type morphology) from 29 inflammatory bowel disease (IBD) patients was performed and compared the expression profile to a control group of 10 sporadic CRCs. Immunohistochemistry for beta-catenin and SATB2 was performed on the 30 CACs. Result LGR5 was positive in 30% (9/30) of CAC cases and 90% (9/10) of sporadic CRCs (p = 0.002). A large majority (89%) of LGR5 positive CACs were of the conventional histologic type, and conventional type CAC showed a significantly higher LGR5 score (median 3.0; interquartile range 1.75–3.25) than non-conventional type CAC (median 1.5; interquartile range 1.00–2.00) (p = 0.034). CAC with conventional morphology did have a lower level of LGR5 expression than sporadic CRC. Sporadic CRCs showed a significantly higher LGR5 level score than non-conventional type CACs (p < 0.001). Nuclear translocation of beta-catenin was strongly associated with LGR5 expression (p = 0.003), however no significant association was identified between SATB2 expression and LGR5 expression status in CACs. Conclusion These findings suggest that the wider spectrum of tumor morphology in CAC may be associated with absence of a LGR5-expressing intestinal stem cell phenotype.

scholarly journals Most colitis associated carcinomas lack expression of LGR5: a preliminary study with implications for unique pathways of carcinogenesis compared to sporadic colorectal carcinoma

2020 ◽  
Author(s):  
Mai Iwaya ◽  
Hiroyoshi Ota ◽  
Tomoyuki Nakajima ◽  
Takeshi Uehara ◽  
Robert Riddell ◽  
...  
Keyword(s):  
Stem Cell ◽  
Colorectal Carcinoma ◽  
Expression Profile ◽  
Intestinal Stem Cell ◽  
Cell Phenotype ◽  
Beta Catenin ◽  
Lgr5 Expression ◽  
Sporadic Crcs ◽  
Conventional Type ◽  
Stem Cell Phenotype

Abstract Background: Leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), a component of the Wnt receptor complex, is thought to lineage label gastric and intestinal stem cells. LGR5 expression is increased in colorectal carcinoma (CRC) compared to normal tissue. Colitis associated colorectal adenocarcinoma (CAC) often shows distinct morphologic and molecular phenotypes compared to sporadic cases. However, the expression profile of LGR5, and by extension the potential role of an intestinal stem cell phenotype, has not been well described in a series of human CAC. Method: RNA in situ hybridization (ISH) for LGR5 expression on 30 CACs (12 cases with conventional morphology and 18 cases with non-conventional type morphology) from 29 inflammatory bowel disease (IBD) patients was performed and compared the expression profile to a control group of 10 sporadic CRCs. Immunohistochemistry for beta-catenin and SATB2 was performed on the 30 CACs. Result: LGR5 was positive in 30% (9/30) of CAC cases and 90% (9/10) of sporadic CRCs (p=0.002). A large majority (89%) of LGR5 positive CACs were of the conventional histologic type, and conventional type CAC showed a significantly higher LGR5 score (median 3.0; interquartile range 1.75-3.25) than non-conventional type CAC (median 1.5; interquartile range 1.00-2.00) (p = 0.034). CAC with conventional morphology did have a lower level of LGR5 expression than sporadic CRC. Sporadic CRCs showed a significantly higher LGR5 level score than non-conventional type CACs (p <0.001). Nuclear translocation of beta-catenin was strongly associated with LGR5 expression (p=0.003), however no significant association was identified between SATB2 expression and LGR5 expression status in CACs. Conclusion: These findings suggest that the wider spectrum of tumor morphology in CAC may be associated with absence of a LGR5 -expressing intestinal stem cell phenotype.

scholarly journals Grain Dispersal Mechanism In Cereals Arose From a Genome Duplication Followed By Changes in Spatial Expression of Genes Involved In Pollen Development

2021 ◽  
Author(s):  
Arthur Cross ◽  
John Baijun ◽  
Robbie Waugh ◽  
Agnieszka Golicz ◽  
Mohammad Pourkheirandish
Keyword(s):  
Expression Profile ◽  
Pollen Development ◽  
Developmental Stages ◽  
Biological Function ◽  
Natural Mode ◽  
Cell Wall Modification ◽  
Spatial Expression ◽  
Dispersal Mechanism ◽  
A Genome ◽  
Ancestral Function

Abstract One of the most critical events in the process of cereal domestication was the loss of the natural mode of grain dispersal. Grain dispersal in barley is controlled by two major genes, Btr1 and Btr2, which affect the thickness of cell walls around the disarticulation zone. The barley genome also encodes Btr1-like and Btr2-like genes, which have been shown to be the ancestral copies. While Btr and Btr-like genes are non-redundant, the biological function of Btr-like genes is unknown. We explored the potential biological role of the Btr-like genes by surveying their expression profile across 212 publicly available transcriptome datasets representing diverse organs, developmental stages and stress conditions. We found that Btr1-like and Btr2-like are expressed exclusively in immature anther samples throughout Prophase I of meiosis within the meiocyte. The similar and restricted expression profile of these two genes suggests they are involved in a common biological function. Further analysis revealed 141 genes co-expressed with Btr1-like and 122 genes co-expressed with Btr2-like, with 105 genes in common, supporting Btr-like genes involvement in a shared molecular pathway. We hypothesize that the Btr-like genes play a crucial role in pollen development by facilitating the formation of the callose wall around the meiocyte or in the secretion of callase by the tapetum. Our data suggest that Btr genes retained an ancestral function in cell wall modification and gained a new role in grain dispersal due to changes in their spatial expression becoming spike specific after gene-duplication.

MicroRNA expression profile and network in colorectal carcinoma after chemotherapy

2010 ◽  
Vol 27 ◽  
pp. S67
Author(s):  
M. Ragusa ◽  
A. Majorana ◽  
L. Statello ◽  
D. Barbagallo ◽  
C. Di Pietro ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Expression Profile ◽  
Microrna Expression ◽  
Microrna Expression Profile

scholarly journals MicroRNAs expression profile in CCR6+ regulatory T cells

2014 ◽  
Author(s):  
Juanjuan Zhao ◽  
Yongju Li ◽  
Yan Hu ◽  
Chao Chen ◽  
Ya Zhou ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Signaling Pathways ◽  
Expression Profile ◽  
Immune Cells ◽  
Potential Role ◽  
Biological Function ◽  
Fold Change ◽  
Insight Into

Backgroud: CCR6+ CD4+ regulatory T cells (CCR6+Tregs), a distinct Tregs subset, played an important role in various immune diseases. Recent evidence showed that microRNAs (miRNAs) are vital regulators in the function of immune cells. However, the potential role of miRNAs in the function of CCR6+Tregs remains largely unknown. In this study, we detected the expression profile of miRNAs in CCR6+ Tregs. Materials and Methods: The expression profile of miRNAs as well as genes in CCR6+Tregs or CCR6-Tregs from Balb/c mice were detected by microarray. The signaling pathways were analyzed using Keggs pathway library. Results: We found that there were 58 miRNAs significantly upregulated and 62 downregulated up to 2 fold in CCR6+Tregs compared with CCR6-Tregs. Moreover, 1391 genes were observed with 3 fold change and 20 signaling pathways were enriched using Keggs pathway library. Conclusion: The present data firstly showed CCR6+Tregs expressed specific miRNAs pattern, which provide an insight into the role of miRNAs in the biological function of distinct Tregs subsets.

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