scholarly journals Filling gaps in the DNA barcode library - Aquatic insects (Ephemeroptera, Plecoptera, Trichoptera) from semi-mountainous and mountainous rivers of Ecoregion 6 (North Macedonia)

2021 ◽  
Vol 4 ◽  
Author(s):  
Biljana Rimcheska ◽  
Yanka Vidinova
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Aquatic Insects ◽  
Molecular Genetic ◽  
Dna Barcode ◽  
Balkan Peninsula ◽  
Molecular Genetic Data ◽  
Medium Quality ◽  
Total Dna ◽  
Data Library

This research provides pivotal molecular genetic data on the community structure of aquatic insects from semi-mountainous and mountainous rivers from the 6th Ecoregion that belongs to the territory of North Macedonia. The aim of this research is to fill the gaps for barcoding the aquatic macroinvertebrates from the Balkan Peninsula and check if the existing barcode library could provide improved identifications for the specimens that were not taxonomically determined to the lowest level possible. We analyzed 95 specimens from which total DNA was extracted and the COI barcode region amplified and sequenced. The taxa were selected from 20 different localities of the territory of western part of North Macedonia. The selected specimens were not determined to species-level in order to test the efficiency of the DNA barcoding methodology and what is missing in the DNA barcoding data library. From the result from one plate (95 specimens) we obtained: 16 samples without barodes, or failed and 10 samples did not have a match in the BOLD database. In the remining 69 samples, three were misidentified. In the total of 69 barcoded species new for the fauna of North Macedonia, 11 are mayflies: Baetis melanonyx, Ecdyonurus vitoshensis, E. macani; stonefly Isoperla vjose; and caddisflies: Agapetus delicatulus, Athripsodes bilineatus, Glossosoma klotho, Lepidostoma basale, Helicopsyche bacescui, Tinodes unicolor and Odontocerum hellenicum. We have also four rarely found species: Zwicknia bifrons, Drussus tenellus, Hydropsyche botosaneanui and Hydropsyche bulbifera, and one species without barcode available as Ecdyonurus sp. SK2 (potential new species). We found 83% efficiency of DNA barcoding, where some samples failed or were with low or medium quality for some specimens, as for the representatives from the genera Baetis, Oxietyra and Rhyacophila. In conclusion we can confirm that 10 of the selected vouchers need to be further identified by morphology and to be added in the BOLD barcode library, and maybe we'll have the possibility to describe a new species as well.

scholarly journals DNA barcode-based survey of Trichoptera in the Crooked River reveals three new species records for British Columbia

2017 ◽  
Author(s):  
Daniel J Erasmus ◽  
Emily A Yurkowski ◽  
Dezene PW Huber
Keyword(s):  
British Columbia ◽  
New Species ◽  
Dna Barcoding ◽  
Dna Barcode ◽  
Geographic Range ◽  
Aquatic Macroinvertebrates ◽  
Aquatic Systems ◽  
Anthropogenic Pressures ◽  
Three New Species ◽  
Occurrence Records

Anthropogenic pressures on aquatic systems have placed a renewed focus on biodiversity of aquatic macroinvertebrates. By combining classical taxonomy and DNA barcoding we identified 39 species of caddisflies from the Crooked River, a unique and sensitive system in the southernmost arctic watershed in British Columbia. Our records include three species never before recorded in British Columbia: Lepidostoma togatum (Lepidostomatidae), Ceraclea annulicornis (Leptoceridae), and Cheumatopsyche harwoodi (Hydropsychidae). Three other specimens may represent new occurrence records and a number of other records seem to be substantial observed geographic range expansions within British Columbia.

scholarly journals DNA barcode-based survey of Trichoptera in the Crooked River reveals three new species records for British Columbia

2017 ◽  
Author(s):  
Daniel J Erasmus ◽  
Emily A Yurkowski ◽  
Dezene PW Huber
Keyword(s):  
British Columbia ◽  
New Species ◽  
Dna Barcoding ◽  
Dna Barcode ◽  
Geographic Range ◽  
Aquatic Macroinvertebrates ◽  
Aquatic Systems ◽  
Anthropogenic Pressures ◽  
Three New Species ◽  
Occurrence Records

Anthropogenic pressures on aquatic systems have placed a renewed focus on biodiversity of aquatic macroinvertebrates. By combining classical taxonomy and DNA barcoding we identified 39 species of caddisflies from the Crooked River, a unique and sensitive system in the southernmost arctic watershed in British Columbia. Our records include three species never before recorded in British Columbia: Lepidostoma togatum (Lepidostomatidae), Ceraclea annulicornis (Leptoceridae), and Cheumatopsyche harwoodi (Hydropsychidae). Three other specimens may represent new occurrence records and a number of other records seem to be substantial observed geographic range expansions within British Columbia.

scholarly journals DNA barcode-based survey of Trichoptera in the Crooked River reveals three new species records for British Columbia

2017 ◽  
Author(s):  
Daniel J Erasmus ◽  
Emily A Yurkowski ◽  
Dezene PW Huber
Keyword(s):  
British Columbia ◽  
New Species ◽  
Dna Barcoding ◽  
Dna Barcode ◽  
Geographic Range ◽  
Aquatic Macroinvertebrates ◽  
Aquatic Systems ◽  
Anthropogenic Pressures ◽  
Three New Species ◽  
Occurrence Records

Anthropogenic pressures on aquatic systems have placed a renewed focus on biodiversity of aquatic macroinvertebrates. By combining classical taxonomy and DNA barcoding we identified 39 species of caddisflies from the Crooked River, a unique and sensitive system in the southernmost arctic watershed in British Columbia. Our records include three species never before recorded in British Columbia: Lepidostoma togatum (Lepidostomatidae), Ceraclea annulicornis (Leptoceridae), and Cheumatopsyche harwoodi (Hydropsychidae). Three other specimens may represent new occurrence records and a number of other records seem to be substantial observed geographic range expansions within British Columbia.

scholarly journals A revision of the green Anoles of Hispaniola with description of eight new species (Reptilia, Squamata, Dactyloidae)

2016 ◽  
pp. 1-135 ◽  
Author(s):  
Gunther Köhler ◽  
S. Blair Hedges
Keyword(s):  
New Species ◽  
Natural History ◽  
Conservation Status ◽  
Molecular Genetic ◽  
Genetic Data ◽  
Species Level ◽  
Zoological Nomenclature ◽  
Distribution Maps ◽  
Dichotomous Key ◽  
Molecular Genetic Data

We revise the species of green anoles (i.e., the species related to Anolis aliniger, A. chlorocyanus, and A. coelestinus) occuring on Hispaniola. Based on our analyses of morphological and molecular genetic data we recognize 16 species of green anoles, eight of which we describe as new species (A. apletolepis sp. nov., A. chlorodius sp. nov., A. divius sp. nov., A. eladioi sp. nov., A. gonavensis sp. nov., A. leucodera sp. nov., A. prasinorius sp. nov. and A. viridius sp. nov.) and three of which are raised from subspecific to species level (A. cyanostictus, A. demissus and A. pecuarius) and one is resurrected from synonymy with A. chlorocyanus (A. peynadoi). Because the six syntypes of A. chlorocyanus (MNHN 785, 787, 2007.2066–09) are conspecific with the only available syntype of A. coelestinus (i.e., MCZ 3347), we have petitioned the International Commission of Zoological Nomenclature (ICZN) to use its plenary power to set aside the type status of the syntypes of Anolis chlorocyanus and to allow the designation of a neotype in order to stabilize the current and long established usage of the names A. chlorocyanus and A. coelestinus. For each species we provide a standardized description of external morphology, color descriptions in life, color photographs in life, description and illustration of hemipenis morphology (if available), distribution maps based on the specimens examined, comments on the conservation status, and natural history notes. Finally, we provide a dichotomous key for the identification of the 16 species of green anoles occuring on Hispaniola.

scholarly journals DNA barcode-based survey of Trichoptera in the Crooked River reveals three new species records for British Columbia

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4221 ◽  
Author(s):  
Daniel J. Erasmus ◽  
Emily A. Yurkowski ◽  
Dezene P.W. Huber
Keyword(s):  
British Columbia ◽  
New Species ◽  
Dna Barcoding ◽  
Dna Barcode ◽  
Geographic Range ◽  
Aquatic Macroinvertebrates ◽  
Aquatic Systems ◽  
Anthropogenic Pressures ◽  
Three New Species ◽  
Occurrence Records

Anthropogenic pressures on aquatic systems have placed a renewed focus on biodiversity of aquatic macroinvertebrates. By combining classical taxonomy and DNA barcoding we identified 39 species of caddisflies from the Crooked River, a unique and sensitive system in the southernmost arctic watershed in British Columbia. Our records include three species never before recorded in British Columbia:Lepidostoma togatum(Lepidostomatidae),Ceraclea annulicornis(Leptoceridae), and possiblyCheumatopsyche harwoodi(Hydropsychidae). Three other specimens may represent new occurrence records and a number of other records seem to be substantial observed geographic range expansions within British Columbia.

A complement to DNA barcoding reference library for identification of fish from the Northeast Pacific

Genome ◽  
2021 ◽  
Author(s):  
Sergei V Turanov ◽  
Yuri Ph. Kartavtsev
Keyword(s):  
Dna Barcoding ◽  
North Pacific Ocean ◽  
Molecular Genetic ◽  
Dna Barcode ◽  
Fish Fauna ◽  
Threshold Value ◽  
Published Data ◽  
Northeast Pacific ◽  
Barcoding Gap ◽  
The North

The seas of the North Pacific Ocean are characterized by a large variety of fish fauna, including endemic species. Molecular genetic methods, often based on DNA barcoding approaches, have been recently used to determine species boundaries and identify cryptic diversity within these species. This study complements the DNA barcode library of fish from the Northeast Pacific area. A library based on 154 sequences of the mitochondrial <i>COI</i> gene from 44 species was assembled and analyzed. It was found that 39 species (89%) can be unambiguously identified by the clear thresholds forming a barcoding gap. Deviations from the standard 2% threshold value resulted in detection of the species <i>Enophrys lucasi </i>in the sample, which is not typical for the eastern part of the Bering Sea. This barcoding gap also made it possible to identify naturally occurring low values of interspecific divergence of eulittoral taxa <i>Aspidophoroides</i> and the deep-sea genus <i>Coryphaenoides</i>. Synonymy of the genus <i>Albatrossia</i> in favor of the genus <i>Coryphaenoides</i> is suggested based on both the original and previously published data.

DNA barcoding unravels three new species and a subspecies of Olepa Watson, 1980 (Lepidoptera, Erebidae, Arctiinae) from India, with morphotypes

2020 ◽  
Vol 19 (2) ◽  
pp. 44-60
Author(s):  
APARNA SURESHCHANDRA KALAWATE ◽  
K. P. DINESH ◽  
A. SHABNAM
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Dna Sequences ◽  
Dna Barcode ◽  
Coi Gene ◽  
Male And Female ◽  
Morphological Variations ◽  
Three New Species ◽  
First Time ◽  
Respective Species

The genus Olepa is distributed in Palearctic and Oriental regions with more species in India and Sri Lanka. In the recent studies, morphological variations within the group were well established, with couple of first set of mt COI DNA barcodes for at least three species. In the present account, three new species and a new subspecies are described from the northern Western Ghats region of Maharashtra based on mt COI DNA barcode studies. Due to high morphological divergence and complete genetic homogeneity on the mt COI DNA, four morphotypes under two species are reported. Morphological and genital characters of male and female are provided along with their respective species morphotypes for the first time under this genus from India. The genitalia and the habitus of male and female are illustrated. Preliminary phylogenetic tree based on the mt COI DNA sequences available in the GenBank for the genus with the sequences for the new species also provided and discussed. Key words: new taxa, morphotype, Maharashtra, DNA barcoding, mt COI gene

Description, DNA barcoding and phylogenetic placement of a remarkable new species of Eopelma (Hymenoptera: Eupelmidae) from Borneo

Zootaxa ◽  
2017 ◽  
Vol 4263 (3) ◽  
Author(s):  
LUCIAN FUSU ◽  
ANDREW POLASZEK
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Citizen Science ◽  
Dna Sequences ◽  
Compound Eye ◽  
Dna Barcode ◽  
Phylogenetic Position ◽  
Phylogenetic Placement ◽  
Chalcid Wasp

Eopelma gibsoni sp. nov. is described based on a male recently collected in Borneo. It is the second species of the genus to be described, and the first species of chalcid wasp in which a pattern of dark stripes on the compound eye is described. The presence of similar dark stripes on the eyes of other chalcid wasps is discussed, highlighting the importance of citizen science. The description is accompanied by a DNA barcode sequence to assist future identification and association of the sexes. The phylogenetic position of E. gibsoni based on 28S DNA sequences is discussed.

scholarly journals BANZARE holothuroids (Echinodermata: Holothuroidea)

Zootaxa ◽  
2009 ◽  
Vol 2196 (1) ◽  
pp. 1-18 ◽  
Author(s):  
P. MARK O’LOUGHLIN
Keyword(s):  
New Species ◽  
New Zealand ◽  
Molecular Genetic ◽  
Genetic Data ◽  
Junior Synonym ◽  
Species Status ◽  
Station Data ◽  
Molecular Genetic Data ◽  
Antarctic Research ◽  
The Antarctic

The holothuroid species collected by The British, Australian and New Zealand Antarctic Research Expedition (BANZARE) are listed, with some systematic annotations. A previous report by O’Loughlin on some BANZARE holothuroids is revised and incorporated. Four new species are described: the Antarctic dactylochirotid Echinocucumis kirrilyae sp. nov.; the Kerguelen dendrochirotid Clarkiella deichmannae sp. nov.; the Antarctic dendrochirotids Trachythyone cynthiae sp. nov. and Trachythyone mackenzieae sp. nov. Cucumaria serrata var. intermedia Théel from Heard and Kerguelen, and Cucumaria serrata var. marionensis Théel from Marion, are raised to species status, and assigned to Pseudocnus Panning. Cucumaria (Semperia) ekmani Ludwig & Heding is a junior synonym of Cucumaria kerguelensis Théel. Cucumaria kerguelensis is re-assigned to Neopsolidium Pawson. Thyone recurvata Théel and Cucumaria squamata Ludwig are junior synonyms of Trachythyone muricata Studer. Cucumaria (Semperia) bouvetensis Ludwig & Heding is formally re-assigned to Trachythyone. Trachythyone baja Hernández is a junior synonym of Trachythyone bouvetensis (Ludwig & Heding). Molecular genetic data indicate possible allopatric cryptic Antarctic forms for the morpho-species Laetmogone wyvillethomsoni Théel. A table with all species and station data is provided.

DNA barcoding confirms species rank for a cryptic geometrid species from Turkey and Bulgaria (Lepidoptera: Geometridae: Sterrhinae)

Zootaxa ◽  
2009 ◽  
Vol 2314 (1) ◽  
pp. 63-68 ◽  
Author(s):  
FEZA CAN
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Male Genitalia ◽  
Dna Barcode

Scopula drenowskii Sterneck, 1941, stat. n. is raised from the synonymy of Scopula decorata to species rank, based on differences in the male genitalia correlated to a considerable genetical distance in the DNA barcode region (COI). The ‘new’ species is known so far from Bulgaria and Turkey.

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