scholarly journals Three new Curvularia species from clinical and environmental sources

MycoKeys ◽  
2020 ◽  
Vol 68 ◽  
pp. 1-21
Author(s):  
Isabel Iturrieta-González ◽  
Josepa Gené ◽  
Nathan Wiederhold ◽  
Dania García
Keyword(s):  
New Species ◽  
Sequence Analysis ◽  
Plant Material ◽  
Novel Species ◽  
Its Region ◽  
Pathogenic Fungi ◽  
Dna Barcodes ◽  
Species Delineation ◽  
Clinical Specimens ◽  
Curvularia Species

Curvularia is a Pleosporalean monophyletic genus with a great diversity of species, including relevant phytopathogenic, animal and human pathogenic fungi. However, their microscopic identification is difficult due to overlapping morphological features amongst species. In recent years, multi-locus sequence analysis using the ITS region of the rDNA and fragments of the genes gapdh and tef1 revealed numerous cryptic species, especially in isolates that commonly produced 3-septate conidia. Therefore, based on sequence analysis of the above-mentioned DNA barcodes recommended for species delineation in Curvularia, we propose three novel species, C. paraverruculosa, C. suttoniae and C. vietnamensis, isolated from soil, human clinical specimens and plant material, respectively, collected in different countries. These new species are morphologically characterised and illustrated in the present study. Curvularia paraverruculosa differs from its counterparts, C. americana and C. verruculosa, mainly by its narrower conidia. Curvularia suttoniae and C. vietnamensis are closely related to C. petersonii, but the former two have larger conidia.

scholarly journals New Species of the Genus Curvularia: C. tamilnaduensis and C. coimbatorensis from Fungal Keratitis Cases in South India

Pathogens ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 9
Author(s):  
Noémi Kiss ◽  
Mónika Homa ◽  
Palanisamy Manikandan ◽  
Arumugam Mythili ◽  
Krisztina Krizsán ◽  
...  
Keyword(s):  
New Species ◽  
Antifungal Susceptibility ◽  
Elongation Factor ◽  
Clinical Importance ◽  
Its Region ◽  
Fungal Keratitis ◽  
Dematiaceous Fungi ◽  
South Indian ◽  
Broth Microdilution Method ◽  
Curvularia Species

Members of the genus Curvularia are melanin-producing dematiaceous fungi of increasing clinical importance as causal agents of both local and invasive infections. This study contributes to the taxonomical and clinical knowledge of this genus by describing two new Curvularia species based on isolates from corneal scrapings of South Indian fungal keratitis patients. The phylogeny of the genus was updated based on three phylogenetic markers: the internal transcribed spacer (ITS) region of the ribosomal RNA gene cluster as well as fragments of the glyceraldehyde-3-phosphate dehydrogenase (gpdh) and translation elongation factor 1-α (tef1α) genes. The maximum likelihood phylogenetic tree constructed from the alignment of the three concatenated loci revealed that the examined isolates are representing two new, yet undescribed, Curvularia species. Examination of colony and microscopic morphology revealed differences between the two species as well as between the new species and their close relatives. The new species were formally described as Curvularia tamilnaduensis N. Kiss & S. Kocsubé sp. nov. and Curvularia coimbatorensis N. Kiss & S. Kocsubé sp. nov. Antifungal susceptibility testing by the broth microdilution method of CLSI (Clinical & Laboratory Standards Institute) revealed that the type strain of C. coimbatorensis is less susceptible to a series of antifungals than the C. tamilnaduensis strains.

Nocardia asiatica sp. nov., isolated from patients with nocardiosis in Japan and clinical specimens from Thailand

2004 ◽  
Vol 54 (1) ◽  
pp. 125-130 ◽  
Author(s):  
Akiko Kageyama ◽  
Natteewan Poonwan ◽  
Katsukiyo Yazawa ◽  
Yuzuru Mikami ◽  
Kazuko Nishimura
Keyword(s):  
Sequence Analysis ◽  
16S Rdna ◽  
Dna Hybridization ◽  
Type Strain ◽  
Novel Species ◽  
Clinical Samples ◽  
Asian Countries ◽  
Clinical Specimens

Five strains isolated from two patients with nocardiosis in Japan and three clinical samples from Thailand were found to have morphological, biochemical and chemotaxonomic properties consistent with their classification in the genus Nocardia. DNA–DNA hybridization, coupled with sequence analysis of 16S rDNA, indicated that these strains belong to a novel species of the genus Nocardia, named Nocardia asiatica sp. nov. because the isolation sites were in Asian countries; the type strain is IFM 0245T (=NBRC 100129T=JCM 11892T=DSM 44668T).

scholarly journals Rhodotorula rosulata sp. nov., Rhodotorula silvestris sp. nov. and Rhodotorula straminea sp. nov., novel myo-inositol-assimilating yeast species in the Microbotryomycetes

2010 ◽  
Vol 60 (10) ◽  
pp. 2501-2506 ◽  
Author(s):  
Wladyslav I. Golubev ◽  
Gloria Scorzetti
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Its Region ◽  
Lsu Rdna ◽  
Rdna Gene ◽  
Physiological Characterization ◽  
Needle Litter

Three novel species are described as Rhodotorula rosulata sp. nov. (type strain VKM Y-2962T =CBS 10977T), Rhodotorula silvestris sp. nov. (type strain VKM Y-2971T =CBS 11420T) and Rhodotorula straminea sp. nov. (type strain VKM Y-2964T =CBS 10976T) based on the study of eight isolates from needle litter. The new species, phylogenetically located within the Microbotryomycetes, are related to glucuronate-assimilating species of the genus Rhodotorula. Sequencing of the D1/D2 domains of the LSU rDNA gene and the internal transcribed spacer (ITS) region, as well as physiological characterization, revealed their distinct taxonomic positions.

Neoerysiphe sechii (Ascomycota: Erysiphales): A new species of powdery mildew found on Sechium edule and Sechium mexicanum (Cucurbitaceae) in Mexico

Botany ◽  
2020 ◽  
Vol 98 (4) ◽  
pp. 185-195 ◽  
Author(s):  
R. Gregorio-Cipriano ◽  
D. González ◽  
R. Félix-Gastélum ◽  
S. Chacón
Keyword(s):  
New Species ◽  
Powdery Mildew ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Its Region ◽  
Specific Primer ◽  
Rdna Sequences ◽  
Sechium Edule ◽  
A New Species

A novel species of powdery mildew, Neoerysiphe sechii sp. nov., has been proposed based on host, morphological characteristics, and gene sequences. The new species was found on chayote (Sechium edule) and a wild species, chayotillo (S. mexicanum), in Veracruz, Mexico. This novel species was identified as a member of Neoerysiphe, based on the morphological characteristics of the anamorph: catenescent conidia, lobed appressoria, and Striatoidium-type conidia. No teleomorph was observed. Neoerysiphe sechii is distinguished by foot cells that are frequently cylindrical with a slight widening at the base, a feature not observed previously in other species in the genus. An Erysiphales-specific primer was designed for the 5′ end of the ITS region of the nuclear rDNA. Independent and concatenated phylogenetic analyses with our rDNA sequences and sequences available in GenBank for Neoerysiphe spp. confirmed that the powdery mildew specimens found in Sechium spp. form an independent and strongly supported lineage. This is the first report of a Neoerysiphe species causing powdery mildew on members of Cucurbitaceae, and it is the second species reported from Mexico after N. cumminsiana. Neoerysiphe sechii is found on cultivated and endemic hosts in Mexico, suggesting that the fungus itself may be endemic.

Diaporthe species occurring on Senna bicapsularis in southern China, with descriptions of two new species

Phytotaxa ◽  
2017 ◽  
Vol 302 (2) ◽  
pp. 145 ◽  
Author(s):  
QIN YANG ◽  
XIN-LEI FAN ◽  
ZHUO DU ◽  
CHENG-MING TIAN
Keyword(s):  
New Species ◽  
Southern China ◽  
Elongation Factor ◽  
Its Region ◽  
Pathogenic Fungi ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Two New Species ◽  
Elongation Factor 1

Diaporthe species include important plant pathogenic fungi with extensive host ranges and geographic distributions. In the present study, two new species of Diaporthe causing diebacks from southern China are described and illustrated. Isolates were characterized morphologically and assigned to species based on multigene sequence analyses. Phylogenetic analysis based on the internal transcribed spacer (ITS) region, calmodulin (CAL), histone H3 (HIS), translation elongation factor 1-alpha (TEF1-α) and beta-tubulin (TUB) region grouped isolates into two distinct lineages. Within each linage these species appear to be unique and are described as D. sennae sp. nov and D. sennicola sp. nov, and their relationships with other species in the genus are indicated.

Phylogeny and taxonomy of the family Arthrodermataceae (dermatophytes) using sequence analysis of the ribosomal ITS region

1999 ◽  
Vol 37 (2) ◽  
pp. 105-114 ◽  
Author(s):  
Y. GRAser ◽  
M. EL Fari ◽  
R. Vilgalys ◽  
A. F. A. Kuijpers ◽  
G. S. DE Hoog ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Its Region ◽  
The Family

scholarly journals Two new species of Undifilum, fungal endophytes of Astragalus (locoweeds) in the United States

Botany ◽  
2012 ◽  
Vol 90 (9) ◽  
pp. 866-875 ◽  
Author(s):  
Deana L. Baucom ◽  
Marie Romero ◽  
Robert Belfon ◽  
Rebecca Creamer
Keyword(s):  
New Species ◽  
New Mexico ◽  
Random Amplified Polymorphic Dna ◽  
Phylogenetic Analyses ◽  
Fungal Endophytes ◽  
Morphological Characteristics ◽  
Its Region ◽  
The United States ◽  
Neighbor Joining

New species of Undifilum , from locoweeds Astragalus lentiginosus Vitman and Astragalus mollissimus Torr., are described using morphological characteristics and molecular phylogenetic analyses as Undifilum fulvum Baucom & Creamer sp. nov. and Undifilum cinereum Baucom & Creamer sp. nov. Fungi were isolated from dried plants of A. lentiginosus var. araneosus , diphysus , lentiginosus , and wahweapensis collected from Arizona, Oregon, and Utah, USA, and A. mollissimus var. biglovii , earleii , and mollissimus collected from New Mexico, Oklahoma, and Texas, USA. Endophytic fungi from Astragalus locoweeds were compared to Undifilum oxytropis isolates obtained from dried plant material of Oxytropis lamberteii from New Mexico and Oxytropis sericea from Arizona, Colorado, New Mexico, Utah, and Wyoming. Extremely slow growth in vitro was observed for all, and conidia, if present, were ellipsoid with transverse septa. However, in vitro color, growth on four different media, and conidium size differed between fungi from Astragalus spp. and U. oxytropis. Neighbor-joining analyses of internal transcribed spacer (ITS) region and glyceraldehyde-3-phosphate dehydrogenase (GPD) gene sequences revealed that U. fulvum and U. cinereum formed a clade distinct from U. oxytropis. This was supported by neighbor-joining analyses of results generated from random amplified polymorphic DNA (RAPD) fragments using two different primers.

scholarly journals Benchmarking DNA barcodes: an assessment using available primate sequences

Genome ◽  
2006 ◽  
Vol 49 (7) ◽  
pp. 851-854 ◽  
Author(s):  
Mehrdad Hajibabaei ◽  
Gregory AC Singer ◽  
Donal A Hickey
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Dna Barcoding ◽  
Species Identification ◽  
Dna Sequences ◽  
Phylogenetic Relationships ◽  
Primate Species ◽  
Dna Barcodes ◽  
Global Biodiversity ◽  
Efficient Sequence

DNA barcoding has been recently promoted as a method for both assigning specimens to known species and for discovering new and cryptic species. Here we test both the potential and the limitations of DNA barcodes by analysing a group of well-studied organisms—the primates. Our results show that DNA barcodes provide enough information to efficiently identify and delineate primate species, but that they cannot reliably uncover many of the deeper phylogenetic relationships. Our conclusion is that these short DNA sequences do not contain enough information to build reliable molecular phylogenies or define new species, but that they can provide efficient sequence tags for assigning unknown specimens to known species. As such, DNA barcoding provides enormous potential for use in global biodiversity studies.Key words: DNA barcoding, species identification, primate, biodiversity.

scholarly journals A revision of the subgenus Dudaica Strand of the genus Drosophila Fallén, with descriptions of six new species (Diptera, Drosophilidae)

ZooKeys ◽  
2018 ◽  
Vol 781 ◽  
pp. 19-50
Author(s):  
Takehiro K. Katoh ◽  
Guang Zhang ◽  
Masanori J. Toda ◽  
Awit Suwito ◽  
Jian-Jun Gao
Keyword(s):  
New Species ◽  
Molecular Data ◽  
Dna Barcodes ◽  
Key To Species ◽  
Morphological And Molecular Data

The subgenus Dudaica Strand of the genus Drosophila Fallén has been known to comprise only two species: Drosophila (Dudaica) senilis Duda, 1926 (recorded from Indonesia, Philippines, Vietnam, Bhutan, and India) and D.malayana (Takada, 1976) (recorded from Malaysia). In the present study, this subgenus is revised, with D.malayana redescribed and six new species discovered and described from China, Malaysia, and Indonesia: gracilipalpis Katoh & Gao, sp. n., puberula Katoh & Gao, sp. n., albipalpis Katoh, Toda & Gao, sp. n., qiongzhouensis Katoh & Gao, sp. n., orthophallata Katoh, Toda & Gao, sp. n., and dissimilis Katoh & Gao, sp. n. Both morphological and molecular data (DNA barcodes) are used to distinguish the above species. A key to species of this subgenus is provided.

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