Morphological and molecular identification of two new Alternaria species (Ascomycota, Pleosporaceae) in section Radicina from China

The fungal genus Alternaria was distributed widely and found in different habitats such as plant or indoor environment. During an investigation into this genus in China, two new Alternaria species, Alternaria vulgarae and A. divaricatae were respectively isolated from diseased leaves of Foeniculum vulgare and Saposhnikovia divaricata, which both belonged to Umbelliferae. Phylogenetically, they were determined as new species belonging in the section Radicina of Alternaria based on the combined four gene fragments of ITS, TEF1, GAPDH and RPB2. Morphologically, the two species were illustrated and compared with other relevant Alternaria species in section Radicina.

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Two new species of planthoppers from India (Hemiptera: Auchenorrhyncha: Delphacidae) in the genera Parasogata and Eoeurysa

European Journal of Taxonomy ◽

10.5852/ejt.2020.724.1161 ◽

2020 ◽

Vol 724 ◽

pp. 93-108

Author(s):

N Ramya ◽

Charles Bartlett ◽

Naresh M. Meshram

Keyword(s):

New Species ◽

Molecular Identification ◽

Gene Sequence ◽

Himachal Pradesh ◽

Key To Species ◽

Northeastern India ◽

Two New Species ◽

Mtcoi Gene

The genus Parasogata Zhou, Yang & Chen, 2018 is here reported from India represented by the new species Parasogata sexpartita sp. nov. collected in a recent exploration and survey of delphacids from Nagaland in northeastern India. A second species of Eoeurysa Muir, 1913 from India, the new species Eoeurysa sagittaria sp. nov., was found in Rampur, Una, Himachal Pradesh. Both new species are described with illustrations, and a molecular identification is given with the mtCOI gene sequence. A modified key to species of the genera is also provided.

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Draft Genome Sequence of Cumin Blight Pathogen Alternaria burnsii

Plant Disease ◽

10.1094/pdis-02-20-0224-a ◽

2020 ◽

Author(s):

Zhonghong Feng ◽

Tom Hsiang ◽

Xiaofei Liang ◽

Rong Zhang ◽

Guangyu Sun

Keyword(s):

Indoor Air ◽

Leaf Spot ◽

Date Palm ◽

Draft Genome ◽

Pumpkin Seed ◽

Diverse Species ◽

Wheat Leaf ◽

Alternaria Species ◽

Seed Rot ◽

Fungal Genus

The fungal genus Alternaria consists of highly diverse species. They can be isolated readily from soil, water and many plants, and even from animals and humans. Alternaria burnsii is a small-spored species of section Alternaria. It has been reported as a pathogen, an endophyte and a saprophyte, and can also be found in indoor air. It causes cumin blight, a destructive disease on cumin (Cuminum cyminum), and also causes other serious diseases, such as pumpkin seed rot, date palm leaf spot, wheat leaf spot, and gray spot of Notopterygium incisum. In this study, we sequenced and assembled the first genome of A. burnsii isolate CBS 107.38. The draft genome can be used as a reference for the further study of related pathogens and comparative genomics of Alternaria species.

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Alternaria telliensis sp. nov., a new species isolated from Solanaceae in Algeria

Phytotaxa ◽

10.11646/phytotaxa.440.2.1 ◽

2020 ◽

Vol 440 (2) ◽

pp. 89-100

Author(s):

NABAHAT BESSADAT ◽

BRUNO HAMON ◽

NELLY BATAILLE-SIMONEAU ◽

KIHAL MABROUK ◽

PHILIPPE SIMONEAU

Keyword(s):

New Species ◽

Elongation Factor ◽

Translation Elongation ◽

Biodiversity Survey ◽

Growing Seasons ◽

Elongation Factor 1 Alpha ◽

Alternaria Species ◽

Pathogenicity Tests ◽

North Western ◽

A New Species

During a biodiversity survey of Alternaria associated with leaf spot and blight of Solanaceae, a large collection of strains was obtained from samples collected in north-western regions of Algeria in 2013–2018 growing seasons. Amongst these strains, three isolates recovered from tomato and potato had morphological traits different from that usually observed for Alternaria species previously reported on Solanaceae. Based on analysis of a sequence dataset corresponding to portions of the glyceraldehyde-3-phosphate dehydrogenase (gpd), translation elongation factor 1-alpha (tef1) and RNA polymerase second largest subunit (rpb2) genes along with morphological observations, isolates were identified as a new species in the section Japonicae. This novel species, described here as Alternaria telliensis, is phylogenetically and morphologically distinct from A. japonica and A. nepalensis in this section. Pathogenicity tests were performed and isolates were found to be weakly pathogenic to tomato and potato (Solanaceae) while highly aggressive on radish, cabbage and turnip (Brassicaceae) plants.

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Morphological and molecular identification of three new species of Tomentella from Finland

Mycologia ◽

10.1080/00275514.2018.1474683 ◽

2018 ◽

Vol 110 (4) ◽

pp. 677-691 ◽

Cited By ~ 1

Author(s):

Xu Lu ◽

Kari Steffen ◽

Hai-Sheng Yuan

Keyword(s):

New Species ◽

Molecular Identification ◽

Three New Species

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Morphological Variability and Molecular Identification ofUncinariaspp. (Nematoda: Ancylostomatidae) from Grizzly and Black Bears: New Species or Phenotypic Plasticity?

Journal of Parasitology ◽

10.1645/14-621.1 ◽

2015 ◽

Vol 101 (2) ◽

pp. 182-192 ◽

Cited By ~ 10

Author(s):

Stefano Catalano ◽

Manigandan Lejeune ◽

Bradley van Paridon ◽

Christopher A. Pagan ◽

James D. Wasmuth ◽

...

Keyword(s):

New Species ◽

Phenotypic Plasticity ◽

Molecular Identification ◽

Morphological Variability ◽

Black Bears

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Morphological and molecular identification of Alternaria hedjaroudei sp. nov., a new species in section Panax from Iran

Phytotaxa ◽

10.11646/phytotaxa.438.2.6 ◽

2020 ◽

Vol 438 (2) ◽

pp. 130-140

Author(s):

ESMAEIL HASHEMLOU ◽

YOUBERT GHOSTA ◽

ALIREZA POURSAFAR ◽

RAZMIG AZIZI

Keyword(s):

New Species ◽

Molecular Identification ◽

Morphological Characteristics ◽

Gene Sequences ◽

Multiple Gene ◽

Sexual Morph ◽

A Chain ◽

Branched Chains ◽

Asexual Morphs ◽

A New Species

Alternaria section Panax currently includes seven species which are characterized by simple or branched and short to moderately long primary conidiophores as well as by solitary to short simple or branched chains of conidia with moderate to relatively long secondary conidiophores. Two species within the section have been reported to have sexual morphs. During the study of fungi on overwintered stems of Serratula coriacea Fisch. & C.A.Mey. (Asteraceae) from different regions of Urmia, West Azarbaijan Province, Iran, 26 isolates (16 from conidia and 10 from single ascospores) representing Alternaria characteristics were isolated and studied taxonomically. All the studied isolates formed sexual morph on PDA, PCA, V8–A, and HA media after 2 months incubation in the dark at 4 ºC and fully matured after 3 months. Based on morphological characteristics of sexual and asexual morphs and multiple gene sequences analyses (ITS-rDNA, GAPDH, RPB2 and TEF), the newly studied isolates represented a new species in Alternaria section Panax, which is described and illustrated here as Alternaria hedjaroudei. Alternaria hedjaroudei is phylogenetically close to A. avenicola, A. calycipyricola and A. photistica but it can be distinguished morphologically based on the formation of short chains of conidia (2–3 in a chain), absence of chlamydospores, smaller conidia size, relatively short secondary conidiophores, and fewer transverse septa in mature ascospores. Detailed morphological comparisons with other species in the section Panax are provided.

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Foeniculum sanguineum Triano & A. Pujadas (Apiaceae) new species from the South-Western Mediterranean Region

Acta Botanica Malacitana ◽

10.24310/actabotanicaabm.v40i0.2548 ◽

2017 ◽

Vol 40 ◽

pp. 71

Author(s):

Antonio J. Pujadas Salvà ◽

Enrique Triano Muñoz ◽

Josefa Anaya ◽

Manuel Grande ◽

César Raposo ◽

...

Keyword(s):

New Species ◽

Mediterranean Region ◽

Phylogenetic Analyses ◽

Diploid Species ◽

Sequence Divergence ◽

Western Mediterranean ◽

Nuclear Ribosomal Dna ◽

Rbcl Gene ◽

The South ◽

Foeniculum Vulgare

English. Foeniculum sanguineum Triano & A. Pujadas, sp. nov., from the south western Mediterranean Region (Spain & Morocco) is described. Its characterization and a comparative study with the related species Foeniculum vulgare Mill., has been carried out through morphological, cytological, chemical and molecular analysis. F. sanguineum is distinguished primarily for its red petals, pink pollen in fresh, and red stylopod. It is a diploid species (2n= 22). A high proportion of limonene and piperitenone oxide (absent in F. vulgare) has been found in the essential oil composition of the dry fruits of F. sanguineum and a high amount (about 50 %) of α-phellandrene in its roots and stems. Phylogenetic analyses were performed using the internal transcribed spacer sequences of nuclear ribosomal DNA (ITS) and the chloroplast rbcL gene sequences. ITS analysis supports the existence of the new species, while revealing sequence divergence both at the intraspecific and at the interspecific levels. A Single-nucleotide-polymorphism (SNP) sequence divergence found in the slow evolving chloroplast gene provided additional support for the novel species characterization, for which the name Foeniculum sanguineum is proposed.Español. Se describe Foeniculum sanguineum Triano & A. Pujadas, sp. nov., del suroeste de la Región Mediterránea (España y Marruecos). Para su caracterización se ha realizado el análisis morfológico, citológico, fitoquímico y molecular. Se ha llevado a cabo el studio comparativo con Foeniculum vulgare Mill. La nueva especie F. sanguineum se distingue principalmente por sus pétalos rojos, polen rosado en fresco y por su estilopodio rojo. Es una especie diploide (2n= 22). Se ha encontrado una alta proporción de óxido de limoneno y piperitenona (ausente en F. vulgare) en la composición de aceite esencial de los frutos secos de F. sanguineum y una elevada cantidad (aproximadamente 50%) de α-felandreno en sus raíces y tallos. El análisis filogenético se realizó utilizando las secuencias del espaciador transcrito interno de ADN ribosomal nuclear (ITS) y las secuencias del gen cloroplástico rbcL. El análisis ITS apoya la existencia de la nueva especie, al tiempo que revela la divergencia de secuencias tanto a nivel intraespecífico como a nivel interespecífico. La divergencia de secuencia encontrada en el gen cloroplástico, aunque reducida a un nucleótido, proporcionó apoyo adicional para la caracterización de la nueva especie, para la que se propone el nombre de Foeniculum sanguineum.

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Molecular identification of genus Pipistrellus (Mammalia: Chiroptera) from Fata region, Pakistan

Brazilian Journal of Biology ◽

10.1590/1519-6984.246322 ◽

2023 ◽

Vol 83 ◽

Author(s):

M. Idnan ◽

A. Javid ◽

M. Tayyab ◽

A. Hussain ◽

S. Mansoor ◽

...

Keyword(s):

New Species ◽

16S Rrna ◽

Molecular Identification ◽

Dna Sequences ◽

Rrna Gene ◽

16S Rrna Gene Sequences ◽

Morphometric Measurements ◽

Tree Analysis ◽

Rrna Sequences ◽

16S Rrna Sequences

Abstract A total of 10 specimens were captured from selected sites of Bajaur Agency FATA, Pakistan using mist nets. The captured specimens were morphologically identified and various morphometric measurements were taken. The head and Body length (HB) of Pipistrellus coromondra and Pipistrellus kuhlii lepidus (n=10) was 43±0.11 mm and 45±1.1 respectively. Morphologically identified Pipistrellus kuhlii confirmed as Pipistrellus kuhlii lepidus based on 16S rRNA sequences. The DNA sequences were submitted to GenBank and accession numbers were obtained (MN 719478 and MT430902). The available 16S rRNA gene sequences of Pipistrellus coromondra and Pipistrellus kuhlii lepidus were retrieved from NCBI and incorporated in N-J tree analysis. Overall, the interspecific genetic variations among Pipistrellus coromondra and Pipistrellus kuhlii lepidus were 8% and 1% respectively. In our recommendation, a comprehensive molecular identification of bats is need of hour to report more cryptic and new species from Pakistan.

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Molecular Identification of Strains of Caridean Species in a Tropical Lagoons of South-Western Nigeria

Journal of Scientific Research ◽

10.3329/jsr.v11i1.37282 ◽

2019 ◽

Vol 11 (1) ◽

pp. 111-120 ◽

Cited By ~ 1

Author(s):

M. F. Akinwunmi ◽

A. O. Lawal-Are

Keyword(s):

New Species ◽

Dna Barcoding ◽

Species Identification ◽

Molecular Identification ◽

Coastal Lagoons ◽

Close Match ◽

Morphological Approach ◽

High Degree ◽

First Time

Species identification by morphological approach requires a high degree of experience, which is difficult and not practical enough for those interested in surveying a broad diversity of organisms. However, molecular or genetic approaches to identify species have been proposed and widely used by most scientists. DNA barcoding was used to identify Macrobrachium species occurring in three coastal lagoons (Badagry, Lagos and Epe) in South-western Nigeria. Samples of prawns for the DNA studies were collected from May to July 2015. The DNA of the Macrobrachium species was extracted using the phenol-chloroform protocol and Norgen tissue kit. Amplification and sequencing of the Macrobrachium species were carried out and further identification was done by comparing with sequenced data in the Genbank. New species of Macrobrachium were obtained in Badagry and Epe Lagoons having a close match with M. asperulum and M. nipponense at 85% and 84% respectively. M. asperulum and M. nipponense are reported in Badagry and Epe Lagoons for the first time.

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