scholarly journals Functional characteristics of lactobacilli from traditional Bulgarian fermented milk products

2019 ◽  
pp. 148-153
Author(s):  
Veronica NEMSKA ◽  
Petya LOGAR ◽  
Tanya RASHEVA ◽  
Zdravka SHOLEVA ◽  
Nelly GEORGIEVA ◽  
...  
Keyword(s):  
Fermented Milk ◽  
Model Systems ◽  
High Survival ◽  
Duration Of Action ◽  
Protection Systems ◽  
Survival Capacity ◽  
Small Intestinal ◽  
Vitro Model ◽  
Probiotic Lactobacilli

After oral administration, probiotic lactobacilli meet a number of protection systems in the human body, such as exposure to gastric, pancreatic, and small intestinal juices. Overcoming these detrimental barriers allows living bacteria to adhere to the intestinal epithelium and permanently colonize the gastrointestinal tract (GIT), providing health benefits to the host. Based on this, the transit tolerance of 25 candidate probiotic lactobacilli from katak, yoghurt, and white-brined and yellow cheese to simulated bile and small intestinal juices of variable pH was investigated. To establish their resistance, in vitro model systems based on modified MRS media and a longer duration of action (up to 24 h of incubation) were designed. Six of the strains studied were found to show strain-specific survival capacity with low viability in conditions simulating stomach acidity and high resistance to bile and intestinal juices. In addition, the adherence capability (autoaggregation and hydrophobicity) of the strains was determined. Obtained results allowed to select Lactobacillus strains with high survival ratios while passing through the GIT and good adherence properties, which make them suitable for the development of new probiotics.

Human 3-dimensional liver organoids: in vitro model systems to study liver diseases

2020 ◽  
Author(s):  
H Gaitantzi ◽  
C Cai ◽  
S Asawa ◽  
K Böttcher ◽  
M Ebert ◽  
...  
Keyword(s):  
Liver Diseases ◽  
In Vitro Model ◽  
Model Systems ◽  
3 Dimensional ◽  
Vitro Model ◽  
Liver Organoids

scholarly journals In Vitro Model Systems for Exploring Oral Biofilms: From Single‐Species Populations to Complex Multi‐Species Communities

2021 ◽  
Author(s):  
Ting L. Luo ◽  
Michael E. Vanek ◽  
Carlos Gonzalez‐Cabezas ◽  
Carl F. Marrs ◽  
Betsy Foxman ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Single Species ◽  
Model Systems ◽  
Oral Biofilms ◽  
Vitro Model

scholarly journals In vitro assessment of the starch digestibility of western Canadian wheat market classes and cultivars

2018 ◽  
Vol 98 (3) ◽  
pp. 463-476 ◽  
Author(s):  
Namalika D. Karunaratne ◽  
Dawn A. Abbott ◽  
Ravindra N. Chibbar ◽  
Pierre J. Hucl ◽  
Curtis J. Pozniak ◽  
...  
Keyword(s):  
Crude Protein ◽  
In Vitro Model ◽  
Starch Digestibility ◽  
Wheat Market ◽  
In Vitro Assessment ◽  
Market Class ◽  
Small Intestinal ◽  
Vitro Model ◽  
Total Starch

The objective of the study was to measure the effect of wheat market class and cultivar on starch digestibility using an in vitro model that mimics the chicken digestive tract and relate it to grain characteristics. The study evaluated 18 wheat cultivars from eight western Canadian wheat classes and, each cultivar was replicated four times. Samples were subjected to gastric and small intestine (SI) digestion phases and each sample was assayed in triplicate; glucose release was measured in SI phase. Starch granule distribution, amylose, total starch, crude protein (CP), ash, and non-starch polysaccharides (NSP) were analyzed in all wheat samples. Small intestinal phase times of 15, 60, and 120 min were chosen to approximate digestion in the terminal duodenum, jejunum, and ileum. Starch digestibility of wheat classes ranged as follows: 15 min — 33.1% to 49.1%, 60 min — 80.2% to 93.3%, and 120 min — 92.4% to 97.6%. Starch digestibility positively correlated with CP, ash, NSP, and proportion of large granules, whereas it negatively correlated with total starch, and proportion of small and medium granules. In conclusion, market class and cultivar of western Canadian wheat affects both rate and extent of starch digestibility and it is related to various grain characteristics.

scholarly journals Effects of uremic solutes on reactive oxygen species in vitro model systems as a possibility of support the renal function management

2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Renata P Assis ◽  
Juliana FA Castro ◽  
Vânia O Gutierres ◽  
Carlos A Arcaro ◽  
Renata S Brotto ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Renal Function ◽  
In Vitro Model ◽  
Reactive Oxygen ◽  
Model Systems ◽  
Oxygen Species ◽  
Uremic Solutes ◽  
Function Management ◽  
Vitro Model

scholarly journals In Vitro Systems for Studying Different Genotypes/Sub-Genotypes of Hepatitis B Virus: Strengths and Limitations

Viruses ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 353 ◽  
Author(s):  
Constance N. Wose Kinge ◽  
Nimisha H. Bhoola ◽  
Anna Kramvis
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
In Vitro Model ◽  
Antiviral Agents ◽  
Model Systems ◽  
Effective Vaccine ◽  
B Virus ◽  
In Vitro Systems ◽  
Vitro Model

Hepatitis B virus (HBV) infects the liver resulting in end stage liver disease, cirrhosis, and hepatocellular carcinoma. Despite an effective vaccine, HBV poses a serious health problem globally, accounting for 257 million chronic carriers. Unique features of HBV, including its narrow virus–host range and its hepatocyte tropism, have led to major challenges in the development of suitable in vivo and in vitro model systems to recapitulate the HBV replication cycle and to test various antiviral strategies. Moreover, HBV is classified into at least nine genotypes and 35 sub-genotypes with distinct geographical distributions and prevalence, which have different natural histories of infection, clinical manifestation, and response to current antiviral agents. Here, we review various in vitro systems used to study the molecular biology of the different (sub)genotypes of HBV and their response to antiviral agents, and we discuss their strengths and limitations. Despite the advances made, no system is ideal for pan-genotypic HBV research or drug development and therefore further improvement is required. It is necessary to establish a centralized repository of HBV-related generated materials, which are readily accessible to HBV researchers, with international collaboration toward advancement and development of in vitro model systems for testing new HBV antivirals to ensure their pan-genotypic and/or customized activity.

Sign in / Sign up

Export Citation Format

Share Document