Correlation between HFE gene polymorphisms and increased risk of coronary artery disease among patients with type 2 diabetes in Iran

Objective: The objective of this study was to investigate the association between proinflammatory cytokines in special, the interleukin-6 (IL-6), and insulin-like growth factor (IGF-1) levels in coronary artery disease (CAD) and type 2 diabetes mellitus (T2DM).Methods: This study was conducted from November 2017 to March 2018 in Anbar, Iraq. We studied a total of 90 individuals (46 men and 44 women) aged between 20 and 87 years. The samples were divided into four groups: CAD patients (n=23), T2DM patients (n=23), coronary artery disease and type 2 diabetes together in the same patient (n=23), and control group (n=21). The concentrations of IL-6 and IGF-1 were determined using a commercially available enzyme-linked immune sorbent assay.Results: The results of the present study showed that there were elevated serum levels of IL-6 and low levels of IGF-1 in all the tested groups, compared with the control. The difference was statistically significant at p<0.05. The results showed a positively correlated between IL-6 and IGF-1 in the CAD group and T2DM group, while it was a negative correlation between serum levels of IL-6 and IGF-1 in the T2DM+CAD group.Conclusion: Elevated levels serum of IL-6 predicts the development of CAD and T2DM. These data support a possible role for inflammation in diabetogenesis and complication of the cardiovascular disease. There is an inverse relationship between the levels serum of IGF-1 and increased risk of CAD and development of T2DM.

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The Relationship Between Adiponectin Serum Level and Coronary Artery Disease in Type 2 Diabetic Patients

ACTA MEDICA IRANICA ◽

10.18502/acta.v59i3.5784 ◽

2021 ◽

Author(s):

Zahra Mazloum Khorasani ◽

Saeed Choobkar ◽

Ramin Khameneh Bagheri ◽

Mina AkbariRad ◽

Abdollah Firoozi

Keyword(s):

Type 2 Diabetes ◽

Coronary Artery Disease ◽

Coronary Artery ◽

Serum Level ◽

Demographic Data ◽

Diabetic Patients ◽

Type 2 Diabetics ◽

Increased Risk ◽

Artery Disease

Adiponectin is an adipocytokine that has a higher serum level in healthy people. In type 2 diabetes, insulin resistance, hypertension, MI, and dyslipidemia, the serum level of adiponectin is lower than 4 µg/mL. Adiponectin is proved to have a protective role against atherosclerotic changes where its low serum levels in type 2 diabetes can lead to the progression of atherosclerotic lesions. In this study, we aimed to survey the possible effects of adiponectin in the development of coronary artery disease in type 2 diabetics. Thirty diabetic cases with coronary artery disease, 30 diabetic cases without known coronary artery disease, and a group of 30 healthy volunteers, all of them were between 18-65-year-old, were entered ourstudy. We gathered demographic data by performing a physical examination followed by filling a checklist and a set of laboratory tests. All the groups were sex and age-matched (P=0.284 and P=0.163 respectively). CAD group had the lowest HBA1C (P<0.001). Both LDL and HDL were also lower in the CAD group (P<0.001). Adiponectin was also lower in the CAD group when compared to other groups (P<0.008) or when compared with only normal diabetics (P<0.002). We found a correlation between adiponectin and HDL (r=0.348, P=0.008), suggesting each unit of reduction in serum level of adiponectin could increase the chance of coronary artery disease by 38% in diabetics. In this study, we showed that the lower serum level of adiponectin is correlated with an increased risk of coronary artery disease in type 2 diabetics.

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TGF-β and FRF-21: association with coronary artery disease in patients with type 2 diabetes and obesity

Obesity and metabolism ◽

10.14341/omet2017338-42 ◽

2017 ◽

Vol 14 (3) ◽

pp. 38-42

Author(s):

Teona A. Shvangiradze ◽

Irina Z. Bondarenko ◽

Ekaterina A. Troshina ◽

Larisa V. Nikankina ◽

Svetlana S. Kukharenko ◽

...

Keyword(s):

Type 2 Diabetes ◽

Coronary Artery Disease ◽

Coronary Artery ◽

Growth Factor ◽

Left Ventricular ◽

Fibroblast Growth Factor 21 ◽

Control Group ◽

Increased Risk ◽

Artery Disease

Backgraund: Obesity and type 2 diabetes mellitus (T2DM) are associated with with an increased risk of cardiovascular disease (CVD) and coronary artery disease (CAD), in particular. Obesity lead to several fibrotic processes, including activation of transforming growth factor (TGF-). Recent data indicate the involvement of Fibroblast growth factor 21 (FGF-21) as an important metabolic regulator, and even biomarker of metabolic changes in obesity and T2DM. Impact of metabolic dysregulation that accompany obesity and T2DM in CAD development remain a great challenge. Aims: To study TGF- and FGF-21 level in patients with obesity and T2DM. Materials and methods: TGF- and FGF-21 were identified in peripheral blood samples of 66 patients with obesity, aged 48-65 years. 1st group included 21 patients with CHD and T2DM; 2nd group (22 patients)- with T2DM and excluded CHD; 3rd group (20 patients) with normal glucose metabolism and excluded CHD. Results: TGF- was lower in patients with CHD (group 1) than in the group of "metabolically healthy" obesity (p=0.022). TGF- in patients with T2DM negatively correlated with LDL cholesterol (r=-0.426, p=0.038) the degree of internal carotid artery stenosis (r=-0.426, p=0.024). Patients with verified CHD had a negative correlation with the processes of heart muscle remodeling (thickness of the left ventricular posterior wall (r=- 0.386, p=0.029) interventricular septum (r=-0.335, p=0.031). All patients with obesity had significantly increased level of FGF-21 compared with the control group (p=0.031) FGF-21 positively correlated with BMI (r=0.473, p=0.033) Conclusions: TGF- has negative correlations with the factors that can influence prognosis and the severity of the CVD/. There were found correlations of FGF-21, TGF- with pathological angiogenesis and changes in normal cardiac geometry in obesity, T2DM and CAD.

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Frequency of TLR 2, 4, and 9 Gene Polymorphisms in Chinese Population and Their Susceptibility to Type 2 Diabetes and Coronary Artery Disease

Journal of Biomedicine and Biotechnology ◽

10.1155/2012/373945 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 25

Author(s):

Fengjing Liu ◽

Weixin Lu ◽

Qiaohui Qian ◽

Weigang Qi ◽

Jifan Hu ◽

...

Keyword(s):

Type 2 Diabetes ◽

Coronary Artery Disease ◽

Coronary Artery ◽

Gene Polymorphisms ◽

Study Groups ◽

Innate Immune ◽

Low Grade ◽

Chinese Han ◽

Artery Disease

Toll-like receptors (TLRs) are pivotal components of the innate immune response. Activation of the innate immune system and subsequent chronic low-grade inflammation are thought to be involved in the pathogenesis of atherosclerosis and type 2 diabetes. In the study, we genotyped TLRs gene polymorphisms, including TLR2 Arg677Trp and Arg753Gln, TLR4 Asp299Gly and Thr399Ile, TLR9-1486T/C and -1237T/C. The frequencies of TT, TC and CC genotype of TLR9-1486T/C mutation were 39.6%, 45.8% and 14.6%, respectively; the frequencies of T allele and C allele were 62.5% and 37.5%. However, neither of these parameters was statistically significant among study groups. In addition, we were surprised to find that the commonly reported TLR SNPs in the Western countries, like TLR2 Arg677Trp or Arg753Gln, TLR4 Asp299Gly or Thr399Ile and TLR9-1237T/C, were not polymorphic at all in all study subjects. In conclusion, our data suggests that TLR2 Arg677Trp or Arg753Gln, TLR4 Asp299Gly or Thr399Ile and TLR9-1237T/C polymorphisms have low frequency and TLR9-1486T/C polymorphism may not be a suitable marker in predicting the susceptibility to type 2 diabetes or coronary artery disease in the Chinese Han population.

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Association of PON1 and APOA5 Gene Polymorphisms in a Cohort of Indian Patients Having Coronary Artery Disease With and Without Type 2 Diabetes

Genetic Testing and Molecular Biomarkers ◽

10.1089/gtmb.2010.0207 ◽

2011 ◽

Vol 15 (7-8) ◽

pp. 507-512 ◽

Cited By ~ 22

Author(s):

Seema Bhaskar ◽

Mala Ganesan ◽

Giriraj Ratan Chandak ◽

Radha Mani ◽

Mohammed M. Idris ◽

...

Keyword(s):

Type 2 Diabetes ◽

Coronary Artery Disease ◽

Coronary Artery ◽

Gene Polymorphisms ◽

Indian Patients ◽

Apoa5 Gene ◽

Artery Disease

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Tag Polymorphisms at the A20 (TNFAIP3) Locus Are Associated With Lower Gene Expression and Increased Risk of Coronary Artery Disease in Type 2 Diabetes

Diabetes ◽

10.2337/db06-0946 ◽

2007 ◽

Vol 56 (2) ◽

pp. 499-505 ◽

Cited By ~ 53

Author(s):

Watip Boonyasrisawat ◽

Delphine Eberle ◽

Simonetta Bacci ◽

Yuan-Yuan Zhang ◽

David Nolan ◽

...

Keyword(s):

Gene Expression ◽

Type 2 Diabetes ◽

Coronary Artery Disease ◽

Coronary Artery ◽

Increased Risk ◽

Artery Disease

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Genetic predisposition to type 2 diabetes is associated with severity of coronary artery disease in patients with acute coronary syndromes

Cardiovascular Diabetology ◽

10.1186/s12933-019-0930-1 ◽

2019 ◽

Vol 18 (1) ◽

Cited By ~ 5

Author(s):

Qiwen Zheng ◽

Jie Jiang ◽

Yong Huo ◽

Dafang Chen

Keyword(s):

Type 2 Diabetes ◽

Coronary Artery Disease ◽

Coronary Artery ◽

Genetic Predisposition ◽

Acute Coronary Syndromes ◽

Genetic Risk Score ◽

Increased Risk ◽

Coronary Syndromes ◽

Artery Disease

Abstract Background Accumulating evidence has shown that type 2 diabetes (T2D) and coronary artery disease (CAD) may stem from a ‘common soil’. The aim of our study was to examine the association between genetic predisposition to T2D and the risk of severe CAD among patients with acute coronary syndromes (ACS) undergoing angiography. Methods The current case–control study included 1414 ACS patients with at least one major epicardial vessel stenosis > 50% enrolled in the ACS Genetic Study. The severity of CAD was quantified by the number of coronary arteries involved. Genetic risk score (GRS) was calculated using 41 common variants that robustly associated with increased risk of T2D in East Asians. Logistic regression models were used to estimate the association between GRS and the severity of CAD. Results In the age-, sex- and BMI-adjusted model, each additional risk allele was associated with a 6% increased risk of multi-vessel disease (OR = 1.06, 95% CI 1.02–1.09). The OR was 1.43 (95% CI 1.08–1.89) for the risk of severe CAD when comparing the extreme tertiles of T2D-GRS. The association was not reduced after further adjustment for conventional cardiovascular risk factors. Additional adjustment for T2D status in our regression model attenuated the association by approximately one quarter. In subgroup analysis, the strengths of the associations between GRS and the severity of CAD were broadly similar in terms of baseline demographic information and disease characteristics. Conclusions Our data indicated that genetic predisposition to T2D is associated with elevated risk of severe CAD. This association revealed a possible causal relationship and is partially mediated through diabetic status.

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Low serum betaine levels as a potential biomarker for type 2 diabetes mellitus in patients with coronary artery disease

10.21203/rs.2.24351/v1 ◽

2020 ◽

Author(s):

Fei Guo ◽

Xueting Qiu ◽

Yuanting Zhu ◽

Zhirong Tan ◽

Dongsheng Ouyang ◽

...

Keyword(s):

Type 2 Diabetes ◽

Coronary Artery Disease ◽

Coronary Artery ◽

Density Lipoprotein ◽

Lower Percentage ◽

Potential Biomarker ◽

Increased Risk ◽

Artery Disease

Abstract Background Dietary betaine intake was reported to associate with improved metabolic profile in type 2 diabetes (T2D). However, the role of circulating betaine in coronary artery disease (CAD) patients with T2D is still unknown. This study aimed to investigate the potential role of serum betaine as a potential biomarker for T2D risk in CAD patients. Methods Total 307 subjects were enrolled with 165 CAD patients (57 with T2D and 108 without) and 142 age and sex matched controls (CON). Fasting serum betaine were detected using liquid chromatography tandem mass spectrometry. Results Serum betaine were lower in simple CAD patients compared with healthy controls, and were further decreased in CAD patients with T2D. Betaine was inversely associated with fasting glucose. Subjects in the highest betaine tertile group had lower triglycerides, total cholesterol and low-density lipoprotein as well was lower percentage of CAD and T2D. Increased betaine in CAD was independently associated with low risk of T2D. Furthermore, betaine was a potential diagnostic marker in distinguishing simple CAD from CAD with T2D. Conclusion Low levels of betaine are associated with increased risk of CAD and T2D in CAD, and could be a biomarker for predicting T2D in CAD.

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The common PPARγ2 Pro12Ala polymorphism is associated with an increased risk of coronary artery disease (CAD) in Iranian patients with type 2 diabetes mellitus

10.21203/rs.3.rs-27376/v1 ◽

2020 ◽

Author(s):

Leila Saremi ◽

Shirin Lotfipanah ◽

Fatemeh Feizy ◽

Fatemeh Rostami Avval ◽

Zohreh Saltanatpour

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Coronary Artery Disease ◽

Coronary Artery ◽

Potential Candidate ◽

Pro12ala Polymorphism ◽

Increased Risk ◽

Artery Disease ◽

Iranian Patients

Abstract Background. Type 2 diabetes (T2DM) is a risk factor for coronary artery disease (CAD) in patients with type 2 diabetes compared with subjects without diabetes. Many studies have been shown that CAD has resulted from the interaction of genetic markers implicated in dyslipidaemia and oxidative stress. The PPARγ gene is considered as a potential candidate gene for the link between diabetes mellitus and CAD in patients with diabetes mellitus. The purpose of the present study was to determine the association of Pro12Ala PPARγ2 polymorphism (rs1801282) with CAD in Iranian patients with T2DM.Methods. We studied 290 unrelated Iranian subjects, including 145 healthy controls and 145 CAD patients with a history of T2DM. Genomic DNA was isolated from peripheral blood, and the PPARγ2 gene mutations were analyzed using the PCR–RFLP technique.Results. Our results revealed a significant difference between the allele frequencies of PPARγ2 Pro12Ala polymorphism between the case and control subjects. However, no significant association was observed between Pro12Ala genotypes and physiologic variables.Conclusion. In summary, it could be concluded that PPARγ2 Pro12Ala polymorphism may be an essential indicator of the increased risk of CAD in diabetic patients among the Iranian population.Trial Registration. This article does not contain any studies with human participants by any of the authors.

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