Evaluation of larval culture and conventional PCR methods for the detection of Strongylus vulgaris in equines of Iran

Food poisoning due to the bacteria is a big global problem in economically and human's health. This problem refers to an illness which is due to infection or the toxin exists in nature and the food that use. Milk is considered a nutritious food because it contains proteins and vitamins. The aim of this study is to detect and phylogeny characterization of staphylococcal enterotoxin B gene (Seb). A total of 200 milk and cheese samples were screened. One hundred ten isolates of Staphylococcus aureus pre-confirmed using selective and differential media with biochemical tests. Genomic DNA was extracted from the isolates and the SEB gene detects using conventional PCR with specific primers. Three staphylococcus aureus isolates were found to be positive for Seb gene using PCR and confirmed by sequencing. Sequence homology showed variety range of identity starting from (100% to 38%). Phylogenetic tree analyses show that samples (6 and 5) are correlated with S. epidermidis. This study discovered that isolates (A6-RLQ and A5-RLQ) are significantly clustered in a group with non- human pathogen Staphylococcus agnetis.

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MICROSCOPIC AND MOLECULAR DIAGNOSIS OF Ascaridia spp. IN DOMESTIC PIGEONS(Columba livia domestica) IN BAGHDAD CITY,IRAQ

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v51i4.1101 ◽

2020 ◽

Vol 51 (4) ◽

pp. 1220-1225

Author(s):

Faraj & Al- Amery

Keyword(s):

Molecular Diagnosis ◽

Columba Livia ◽

Ascaridia Galli ◽

Molecular Assay ◽

Infection Rates ◽

Conventional Pcr ◽

Significant Difference ◽

Pcr Products ◽

Males And Females ◽

Domestic Pigeons

Ascaridiosis is a very important parasitic disease of birds, it is caused by Ascaridia. This study was conducted to identify the Ascaridia species by microscopic and molecular assay in Baghdad city. One hundred and sixty fecal samples were collected from domestic pigeons during the period from 1/1/ 2019 to 31/3/ 2019. Results showed that the rate of infection for Ascaridia spp. 15.62% by microscopic examination. Significant difference was observed in infection rates between males and females pigeons. Fifty samples randomly selected and subjected to molecular diagnosis of Ascaridia spp.. Molecular examination results, the total infection rate showed 16%(8/50). The eight positive PCR products were sequenced and deposited in Gene bank data base, phylogenic analysis demonstrated that 4 sequences belongs to Ascaridia galli ( MK918635.1, MK918636.1, MK918847.1, MK919081.1), while 2 (MK919199.1, MK919200.1) belong to Ascaridia nymphii and 2 (MK919207.1, MK919264.1) belong to Ascaridia numidae. It is the first study in Iraq to diagnosis of Ascaridia nymphii and Ascaridia numidae in domesticed pigeons by using conventional PCR.

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Comparative Value of Real Time and Conventional PCR in Antenatal Gender Determination

Bulletin of Egyptian Society for Physiological Sciences ◽

10.21608/besps.2016.8643 ◽

2016 ◽

Vol 36 (1) ◽

pp. 30-41

Author(s):

Naglaa Azab ◽

Rabab Salim ◽

Mohamed Farag

Keyword(s):

Real Time ◽

Conventional Pcr ◽

Gender Determination

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Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species

Journal of Clinical Microbiology ◽

10.1128/jcm.02883-13 ◽

2013 ◽

Vol 52 (2) ◽

pp. 557-563 ◽

Cited By ~ 29

Author(s):

X. Wang ◽

D. J. Seo ◽

M. H. Lee ◽

C. Choi ◽

A. B. Onderdonk

Keyword(s):

Multiplex Pcr ◽

Rapid Detection ◽

Isothermal Amplification ◽

Conventional Pcr ◽

Loop Mediated Isothermal Amplification ◽

Pcr Multiplex

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The production of excretory-secretory molecules from Heligmosomoides polygyrus bakeri fourth stage larvae varies between mixed and single sex cultures

Parasites & Vectors ◽

10.1186/s13071-021-04613-9 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Marta Maruszewska-Cheruiyot ◽

Ludmiła Szewczak ◽

Katarzyna Krawczak-Wójcik ◽

Magdalena Głaczyńska ◽

Katarzyna Donskow-Łysoniewska

Keyword(s):

Sex Pheromones ◽

Pheromone Communication ◽

Larval Culture ◽

Heligmosomoides Polygyrus ◽

Single Sex ◽

Opposite Sex ◽

Potential Vaccine ◽

Immunomodulatory Properties ◽

Therapeutic Compounds ◽

Immunomodulatory Potential

Abstract Background Excretory-secretory (ES) products are crucial in maintaining helminths in the host. Consequently, the proteins of ES are potential vaccine molecules and potential therapeutic agents for autoimmune diseases. Heligmosomoides polygyrus bakeri, a gastrointestinal parasite of mice, is a model of hookworm infection in humans. ES produced by both sexes of H. polygyrus bakeri L4 stage cultured separately shows different immunomodulatory properties than ES obtained when both sexes are cultured together. Accordingly, the objective of this study was to identify and compare the excretory-secretory molecules from single-sex and mixed cultures. Methods The composition of ES of male and female L4 stage nematodes in the presence (cultured together) or absence (cultured alone) of the opposite sex was examined. Proteins were identified using mass spectrometry. The functions of identified proteins were explored with Blast2GO. Results A total of 258 proteins derived from mixed larval culture in the presence of sex pheromones were identified, 160 proteins from pure female cultures and 172 from pure male cultures. Exposure of nematodes to the sex pheromones results in abundant production of proteins with immunomodulatory properties such as Val proteins, acetylcholinesterases, TGF-β mimic 9 and HpARI. Proteins found only in ES from mixed larval cultures were TGF-β mimics 6 and 7 as well as galectin. Conclusions The presence of the opposite sex strongly influences the composition of ES products, probably by chemical (pheromone) communication between individuals. However, examination of the composition of ES from various conditions gives an opportunity for searching for new potentially therapeutic compounds and anthelminthics as well as components of vaccines. Manipulation of the nematode environment might be important for the studies on the immunomodulatory potential of nematodes.

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