The effects of medium composition and culture conditions on in vitro rooting and ex vitro establishment of jackfruit (Artocarpus heterophyllus Lam.)

The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%), but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%). However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution.

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An Efficient Method for In Vitro Shoot-Tip Culture and Sporophyte Production Using Selaginella martensii Spring Sporophyte

Plants ◽

10.3390/plants9020235 ◽

2020 ◽

Vol 9 (2) ◽

pp. 235 ◽

Cited By ~ 1

Author(s):

Kyungtae Park ◽

Bo Kook Jang ◽

Ha Min Lee ◽

Ju Sung Cho ◽

Cheol Hee Lee

Keyword(s):

Nitrogen Sources ◽

Culture Conditions ◽

Shoot Tips ◽

Shoot Tip ◽

Soil Conditions ◽

Ms Medium ◽

Ex Vitro ◽

Shoot Tip Culture ◽

Propagation Methods

Selaginella martensii, an evergreen perennial fern that is native to South America and New Zealand, is named “frosty fern” because of its beautiful white-colored leaves and it is used as an ornamental plant. Efficient propagation methods for this species have not been developed. We aimed to develop an efficient propagation method for S. martensii through in vitro culture. We investigated culture conditions that are suitable for shoot-tip proliferation and growth. The optimum shoot-tip culture conditions were determined while using Murashige and Skoog (MS) medium (quarter, half, full, or double strength) and macronutrients (sucrose and two nitrogen sources) at various concentrations. In MS medium, the shoot tips formed a maximum of 6.77 nodes per explant, and each node formed two new shoot tips (i.e., 26 or 64 shoot tips). When using branching segments containing an angle meristem, the shoot-to-rhizophore formation ratio could be controlled by medium supplementation with plant-growth regulators. Sporophytes that were grown from shoot tips in vitro were acclimated in ex vitro soil conditions and successfully survived in the greenhouse. Numerous shoot tips could be obtained from in vitro-grown sporophytes and be proliferated ex vitro to produce a large number of plants. This method provides a way of shortening the time that is required for producing a large stock of S. martensii planting material.

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Physiological Response of In Vitro Cultured MAGNOLIASP. to Nutrient Medium Composition

Journal of Horticultural Research ◽

10.2478/johr-2014-0006 ◽

2014 ◽

Vol 22 (1) ◽

pp. 49-61 ◽

Cited By ~ 8

Author(s):

Rossen S. Sokolov ◽

Bistra Y. Atanassova ◽

Elena T. Iakimova

Keyword(s):

Nutrient Medium ◽

Culture Conditions ◽

Medium Composition ◽

Naphthaleneacetic Acid ◽

Axillary Shoots ◽

Regeneration Rate ◽

Primary Explants ◽

High Regeneration ◽

Basal Salts

AbstractThe objective of this study was to assess the regeneration response of in vitro cultured Magnolia × soulangeana ‘Alexandrina’ and Magnolia liliiflora ‘Nigra’ to nutrient medium composition. In the primary culture (initiated from dormant axillary buds) combinations of Murashige and Skoog (MS) basal salts with 6-benzylaminopurine and α-naphthaleneacetic acid were tested. The primary explants of cv. ‘Alexandrina’ expressed higher regeneration rate than cv. ‘Nigra’. For both species, the regen eration was most strongly potentiated at addition of 0.25 mg dm−3 of the cytokinin alone. The auxin exerted undesir–able effects. Several basal salts media were applied in proliferation stage and their physiological effects were evaluated in reference to traditionally used MS. At culturing on Chée & Pool C2d Vitis Medium (VM) that is for the first time introduced to magnolia and on MS, M. liliiflora formed more but less elongated shoots than M. soulangeana. However, on VM, substantial increase (25-30%) of the number of axillary shoots and leaves, shoot length and fresh and dry weights over MS was established for both species. This suggested VM as promising composition of nutrients in multiplication stage. Microshoots obtained on MS, VM, Rugini Olive Medium and DKW Juglans Medium were successfully rooted in vitro and subsequently established ex vitro. The findings expand the information on magnolia response to culture conditions and contribute to elaboration of innovative elements of protocols for establishing tissue cultures with high regeneration capacity.

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The Effect of In Vitro Culture Conditions on the Pattern of Photoinhibition during Acclimation of Gardenia Plantlets to Ex Vitro Conditions

Photosynthetica ◽

10.1023/a:1012443919022 ◽

2001 ◽

Vol 39 (1) ◽

pp. 67-73 ◽

Cited By ~ 14

Author(s):

M.D. Serret ◽

M.I. Trillas ◽

J.L. Araus

Keyword(s):

In Vitro Culture ◽

Culture Conditions ◽

Ex Vitro

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A COMPREHENSIVE DESCRIPTION OF EX-VITRO ROOTING OF PISTACHIO ROOTSTOCKS GROWN IN PHOTOMIXOTROPHIC AND PHOTOAUTOTROPHIC CONDITION

FRUIT GROWING RESEARCH ◽

10.33045/fgr.v36.2020.08 ◽

2020 ◽

Vol 36 ◽

pp. 66-74

Author(s):

Mohammad Javad Mahmoudi Meimand ◽

Mohammad Hossein Shamshiri ◽

Khalil Malekzadeh ◽

Mohammad Reza Dehghani

Keyword(s):

Sucrose Concentration ◽

Ex Vitro Rooting ◽

In Vitro Rooting ◽

Naphthalene Acetic Acid ◽

Ex Vitro ◽

Indole Butyric Acid ◽

Micro Propagation ◽

The Media ◽

Photoautotrophic Condition

As a basic principle, ex-vitro rhizogenesis increases the micropropagation efﬁciency of Micro propagation in any plant from both biological and economic viewpoints. In the current study, we surveyed the effects of number of air exchanges along with sucrose concentration on in-vitro rooting of two pistachio rootstocks consisting of UCB1 and Qazvini versus ex-vitro rooting. Based on our findings for the UCB1 rootstock, microshoot ex-vitro rooting reached the highest percentage (63.70 %) after six weeks' treatment with indole butyric acid (IBA) (5000 ppm) and free naphthalene acetic acid (NAA), while for Qazvini rootstock treated with NAA (6000 ppm) along with IBA (5000 ppm), rooting achieved 35.06%. Photomixotrophic resulted from decreasing sucrose concentration from 30 to 15 (g L-1) in corporation with ventilation condition increased UCB1 rooting (67.89%) as well as plant survival (58.34%). For Qazvini rootstock, maximum sucrose concentration (30 g L-1) improved rooting parameters. For in-vitro rooting experiment, rooting percentage of UCB1 plantlets as well as the main and lateral produced roots were higher in media supplemented with (1 mg l-1) IBA, free NAA, and BA. Regarding Qazvini rootstock, the highest in-vitro rooting percentage (43.75%) and root length were associated with the media supplemented with BA (0.5 mg l-1), IBA (2 mg l-1), and NAA (2 mg l-1). As a result, for both the studied rootstocks, better rooting parameters were observed in the ex-vitro rooted microshoots than in-vitro rooted.

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EFFECTS OF CARBON SOURCE, CARBON CONCENTRATION AND CULTURE CONDITIONS ON IN VITRO ROOTING OF PINUS PINEA L. MICROSHOOTS

Acta Horticulturae ◽

10.17660/actahortic.2009.812.19 ◽

2009 ◽

pp. 173-180 ◽

Cited By ~ 8

Author(s):

A. Zavattieri ◽

M. Lima ◽

V. Sobral ◽

P. Oliveira ◽

A. Costa

Keyword(s):

Carbon Source ◽

Carbon Concentration ◽

Culture Conditions ◽

Pinus Pinea ◽

In Vitro Rooting

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Rooting Medium Composition and In Vitro Rooting and Greenhouse Survival of Five Raspberry Cultivars

HortScience ◽

10.21273/hortsci.39.4.788c ◽

2004 ◽

Vol 39 (4) ◽

pp. 788C-788

Author(s):

Becky R. Hughes* ◽

Wanda J. Cook ◽

Candy N.F. Keith

Keyword(s):

Dry Weight ◽

Root Diameter ◽

Medium Composition ◽

Rubus Idaeus ◽

In Vitro Rooting ◽

Stem Length ◽

Indolebutyric Acid ◽

Rooting Medium ◽

Plant Stem Length

In vitro rooting and subsequent greenhouse survival of `Autumn Britten', `Boyne', `Comet',`Nova' and `Qualicum' raspberry (Rubus idaeus L.) plantlets were compared following four weeks on a rooting medium with and without activated charcoal, and with 0.1, 0.5, 1.0, 2.0 or 3.0 milligrams per litre IBA. The addition of charcoal significantly increased the percentage of plantlets that produced roots in vitro for the hard-to-root cultivars. Percent rooting in vitro was highest with the three lower levels of IBA. Root number was influenced only by the cultivar, while root diameter and length were affected by all the factors investigated. Greenhouse survival was affected by the cultivar, the presence or absence of charcoal and the IBA level in the in vitro rooting medium, with significant interactions. Provided charcoal was present in the rooting medium, the level of IBA didn`t alter survival. The addition of charcoal to the rooting medium improved greenhouse survival of the three hardest-to-root cultivars. Plug plant stem length; internode length and dry weight were increased by the presence of charcoal in the in vitro rooting medium for all but the easiest to establish cultivar. Chemical names used: 3-indolebutyric acid (IBA).

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