Molecular Characterization of Genetic Diversity and Structure in South Indian Musa Cultivars

2011 ◽  
Vol 7 (4) ◽  
pp. 274-282 ◽  
Author(s):  
L. Resmi ◽  
Ratna Kumari ◽  
K.V. Bhat ◽  
Ashalatha S. Nair
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
South Indian ◽  
Genetic Diversity And Structure

Molecular characterization of echovirus 13 uncovering high genetic diversity and identification of new genotypes in Pakistan

2017 ◽  
Vol 48 ◽  
pp. 102-108
Author(s):  
Shahzad Shaukat ◽  
Mehar Angez ◽  
Tariq Mahmood ◽  
Muhammad Masroor Alam ◽  
Salmaan Sharif ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
High Genetic Diversity

scholarly journals Molecular Characterization of Pathogenicity Locus (PaLoc) and tcdC Genetic Diversity Among tcdA+B+ Clostridioides Difficile Clinical Isolates in Tehran, Iran

2020 ◽  
Author(s):  
Mansoor Kodori ◽  
Zohreh Ghalavand ◽  
Abbas Yadegar ◽  
Gita Eslami ◽  
Masoumeh Azimirad ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Disease Severity ◽  
Genetic Variants ◽  
Rflp Analysis ◽  
Clostridioides Difficile ◽  
Pcr Rflp ◽  
The Common ◽  
Healthcare Associated

Abstract Background: Clostridioides difficile is the main cause of healthcare-associated diarrhea worldwide. It is proposed that certain C. difficile toxinotypes with distinct pathogenicity locus (PaLoc) variants are associated with disease severity and outcomes. Additionally, few studies have described the common C. difficile toxinotypes, and also little is known about the tcdC variants in Iranian isolates. We characterized the toxinotypes and the tcdC genotypes from a collection of Iranian clinical C. difficile tcdA+B+ isolates with known ribotypes (RTs).Methods: Fifty C. difficile isolates with known RTs and carrying the tcdA and tcdB toxin genes were analyzed. Toxinotyping was carried out based on a PCR-RFLP analysis of a 19.6 kb region encompassing the PaLoc. Genetic diversity of the tcdC gene was determined by the sequencing of the gene.Results: Of the 50 C. difficile isolates investigated, five distinct toxinotypes were recognized. Toxinotypes 0 (33/50, 66%) and V (11/50, 22%) were the most frequently found. C. difficile isolates of the toxinotype 0 mostly belonged to RT 001 (12/33, 36.4%), whereas toxinotype V consisted of RT 126 (9/11, 81.8%). The tcdC sequencing showed six variants (35/50, 70%); tcdC-sc3 (24%), tcdC-A (22%), tcdC-sc9 (18%), tcdC-B (2%), tcdC-sc14 (2%), and tcdC-sc15 (2%). The remaining isolates were wild-types (15/50, 30%) in the tcdC gene.Conclusions: The present study demonstrates that the majority of clinical tcdA+B+ isolates of C. difficile frequently harbor tcdC genetic variants. We also found that the RT 001/ toxinotype 0 and the RT 126/ toxinotype V are the most common types among Iranian isolates. Further studies are needed to investigate the putative association of various tcdC genotypes with CDI severity and its recurrence.

Molecular characterization of genetic diversity and similarity centers of safflower accessions with ISSR markers

2020 ◽  
Vol 43 (1) ◽  
pp. 109-121 ◽  
Author(s):  
Fawad Ali ◽  
Muhammad Azhar Nadeem ◽  
Ephrem Habyarimana ◽  
Abdurrahim Yılmaz ◽  
Muhammad Amjad Nawaz ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Issr Markers

Genetic diversity and molecular characterization of Babesia motasi -like in small ruminants and ixodid ticks from China

2016 ◽  
Vol 41 ◽  
pp. 8-15 ◽  
Author(s):  
Qingli Niu ◽  
Zhijie Liu ◽  
Jifei Yang ◽  
Peifa Yu ◽  
Yuping Pan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Small Ruminants ◽  
Ixodid Ticks

scholarly journals Molecular Characterization of Integrons in Epidemiologically Unrelated Clinical Isolates of Acinetobacter baumannii from Italian Hospitals Reveals a Limited Diversity of Gene Cassette Arrays

2002 ◽  
Vol 46 (11) ◽  
pp. 3665-3668 ◽  
Author(s):  
Francesca Gombac ◽  
Maria Letizia Riccio ◽  
Gian Maria Rossolini ◽  
Cristina Lagatolla ◽  
Enrico Tonin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Acinetobacter Baumannii ◽  
Molecular Characterization ◽  
Horizontal Transfer ◽  
Gene Cassette ◽  
Clinical Isolates

ABSTRACT Integron carriage by 36 epidemiologically unrelated Acinetobacter baumannii isolates collected over an 11-year period from patients in six different Italian hospitals was investigated. Sixteen type 1 integron-positive isolates (44%) were found, 13 of which carried the same array of cassettes, i.e., aacC1, orfX, orfX′, and aadA1a. As ribotype analysis of the isolates demonstrated a notable genetic diversity, horizontal transfer of the entire integron structure or ancient acquisition was hypothesized.

Molecular characterization of norovirus variants and genetic diversity of noroviruses and sapoviruses in Thailand

2013 ◽  
Vol 86 (7) ◽  
pp. 1210-1218 ◽  
Author(s):  
Natthawan Chaimongkol ◽  
Pattara Khamrin ◽  
Rungnapa Malasao ◽  
Aksara Thongprachum ◽  
Tipachan Kongsricharoern ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization

scholarly journals Short Note: Development and characterization of 16 new polymorphic microsatellite loci for Schima superba (Theaceae)

2013 ◽  
Vol 62 (1-6) ◽  
pp. 124-126 ◽  
Author(s):  
Hong-Yu Niu ◽  
Wan-Hui Ye ◽  
Zheng-Feng Wang ◽  
Ying Chen ◽  
Hong-Lin Cao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Large Scale ◽  
Short Note ◽  
Schima Superba ◽  
Broad Leaved Forest ◽  
Genetic Diversity And Structure ◽  
Scale Structures ◽  
Polymorphic Microsatellite ◽  
Leaved Forest

Abstract Schima superba is a common dominant tree species in evergreen broad-leaved forest in subtropical China. Despite its multiple usages in wood industry, reforestation and traditional Chinese medicine, its genetic diversity is poorly studied. To help studying its genetic diversity and structure in the future, after microsatellite enrichment and screening, we identified 16 microsatellites in S. superba. These markers showed polymorphism in three populations. The number of alleles per locus ranged from 3 to 32 with a mean of 14. Within populations, the observed and unbiased expected heterozygosities ranged from 0.048 to 0.926 and from 0.048 to 0.949, respectively. The newly developed 16 microsatellites will be useful for investigating the genetic diversity and structure from large scale patterns to fine-scale structures in this species.

scholarly journals Development and characterization of 24 polymorphic microsatellitelocifor the freshwater fishIchthyoelephas longirostris(Characiformes: Prochilodontidae)

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2419 ◽  
Author(s):  
Ricardo M. Landínez-García ◽  
Edna J. Márquez
Keyword(s):  
Genetic Diversity ◽  
Migratory Species ◽  
Genetic Stock ◽  
Genetic Studies ◽  
Genetic Diversity And Structure ◽  
Polymorphic Microsatellite ◽  
First Time ◽  
Management And Conservation ◽  
Generation Sequencing

The Neotropical freshwater fishIchthyoelephas longirostris(Characiformes: prochilodontidae) is a short-distance migratory species endemic to Colombia. This study developed for the first time a set of 24 polymorphic microsatellitelociby using next-generation sequencing to explore the population genetics of this commercially exploited species. Nineteen of theselociwere used to assess the genetic diversity and structure of 193I. longirostrisin three Colombian rivers of the Magdalena basin. Results showed that a single genetic stock circulates in the Cauca River, whereas other single different genetic stock is present in the rivers Samaná Norte and San Bartolomé-Magdalena. Additionally,I. longirostriswas genetically different among and across rivers. This first insight about the population genetic structure ofI. longirostrisis crucial for monitoring the genetic diversity, the management and conservation of its populations, and complement the genetic studies in Prochilodontidae.

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